Transcriptional Activation of Biosynthetic Gene Clusters in Filamentous Fungi
Filamentous fungi are highly productive cell factories, many of which are industrial producers of enzymes, organic acids, and secondary metabolites. The increasing number of sequenced fungal genomes revealed a vast and unexplored biosynthetic potential in the form of transcriptionally silent seconda...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.901037/full |
| _version_ | 1818529471676809216 |
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| author | László Mózsik Riccardo Iacovelli Roel A. L. Bovenberg Roel A. L. Bovenberg Arnold J. M. Driessen |
| author_facet | László Mózsik Riccardo Iacovelli Roel A. L. Bovenberg Roel A. L. Bovenberg Arnold J. M. Driessen |
| author_sort | László Mózsik |
| collection | DOAJ |
| description | Filamentous fungi are highly productive cell factories, many of which are industrial producers of enzymes, organic acids, and secondary metabolites. The increasing number of sequenced fungal genomes revealed a vast and unexplored biosynthetic potential in the form of transcriptionally silent secondary metabolite biosynthetic gene clusters (BGCs). Various strategies have been carried out to explore and mine this untapped source of bioactive molecules, and with the advent of synthetic biology, novel applications, and tools have been developed for filamentous fungi. Here we summarize approaches aiming for the expression of endogenous or exogenous natural product BGCs, including synthetic transcription factors, assembly of artificial transcription units, gene cluster refactoring, fungal shuttle vectors, and platform strains. |
| first_indexed | 2024-12-11T17:07:18Z |
| format | Article |
| id | doaj.art-497be4fc9edb47a28bf9a0a2a3eb8d22 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-11T17:07:18Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-497be4fc9edb47a28bf9a0a2a3eb8d222022-12-22T00:57:39ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-07-011010.3389/fbioe.2022.901037901037Transcriptional Activation of Biosynthetic Gene Clusters in Filamentous FungiLászló Mózsik0Riccardo Iacovelli1Roel A. L. Bovenberg2Roel A. L. Bovenberg3Arnold J. M. Driessen4Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, NetherlandsDepartment of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, NetherlandsDSM Biotechnology Center, Delft, NetherlandsDepartment of Synthetic Biology and Cell Engineering, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, NetherlandsDepartment of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, NetherlandsFilamentous fungi are highly productive cell factories, many of which are industrial producers of enzymes, organic acids, and secondary metabolites. The increasing number of sequenced fungal genomes revealed a vast and unexplored biosynthetic potential in the form of transcriptionally silent secondary metabolite biosynthetic gene clusters (BGCs). Various strategies have been carried out to explore and mine this untapped source of bioactive molecules, and with the advent of synthetic biology, novel applications, and tools have been developed for filamentous fungi. Here we summarize approaches aiming for the expression of endogenous or exogenous natural product BGCs, including synthetic transcription factors, assembly of artificial transcription units, gene cluster refactoring, fungal shuttle vectors, and platform strains.https://www.frontiersin.org/articles/10.3389/fbioe.2022.901037/fullsecondary metabilitesbiosynthetic gene clustersynthetic biologysynthetic transcriptional regulatorsfungal platform strains |
| spellingShingle | László Mózsik Riccardo Iacovelli Roel A. L. Bovenberg Roel A. L. Bovenberg Arnold J. M. Driessen Transcriptional Activation of Biosynthetic Gene Clusters in Filamentous Fungi Frontiers in Bioengineering and Biotechnology secondary metabilites biosynthetic gene cluster synthetic biology synthetic transcriptional regulators fungal platform strains |
| title | Transcriptional Activation of Biosynthetic Gene Clusters in Filamentous Fungi |
| title_full | Transcriptional Activation of Biosynthetic Gene Clusters in Filamentous Fungi |
| title_fullStr | Transcriptional Activation of Biosynthetic Gene Clusters in Filamentous Fungi |
| title_full_unstemmed | Transcriptional Activation of Biosynthetic Gene Clusters in Filamentous Fungi |
| title_short | Transcriptional Activation of Biosynthetic Gene Clusters in Filamentous Fungi |
| title_sort | transcriptional activation of biosynthetic gene clusters in filamentous fungi |
| topic | secondary metabilites biosynthetic gene cluster synthetic biology synthetic transcriptional regulators fungal platform strains |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.901037/full |
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