UV cross-linked polyvinylpyrrolidone electrospun fibres as antibacterial surfaces
Many bacteria become progressively more resistant to antibiotics and it remains a challenging task to control their overall levels. Polymers combined with active biomolecules come to the forefront for the design of antibacterial materials that can address this encounter. In this work, we investigate...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-12-01
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| Series: | Science and Technology of Advanced Materials |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/14686996.2019.1667737 |
| _version_ | 1818130723302801408 |
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| author | Barbara M. Maciejewska Jacek K. Wychowaniec Marta Woźniak-Budych Łukasz Popenda Alicja Warowicka Klaudia Golba Jagoda Litowczenko Zbigniew Fojud Beata Wereszczyńska Stefan Jurga |
| author_facet | Barbara M. Maciejewska Jacek K. Wychowaniec Marta Woźniak-Budych Łukasz Popenda Alicja Warowicka Klaudia Golba Jagoda Litowczenko Zbigniew Fojud Beata Wereszczyńska Stefan Jurga |
| author_sort | Barbara M. Maciejewska |
| collection | DOAJ |
| description | Many bacteria become progressively more resistant to antibiotics and it remains a challenging task to control their overall levels. Polymers combined with active biomolecules come to the forefront for the design of antibacterial materials that can address this encounter. In this work, we investigated the photo-crosslinking approach of UV-sensitive benzophenone molecule (BP) with polyvinylpyrrolidone (PVP) polymer within electrospun fibres. The BP and PVP solutions allowed fabricating polymer mats that were subsequently functionalised with antibacterial lysozyme. The physical properties of the crosslinked electrospun fibres were investigated by scanning electron microscopy and atomic force microscopy. The average diameter of the obtained fibres decreased from 290 ± 50 nm to 270 ± 70 nm upon the addition of the crosslinking molecules and then to 240 ± 80 nm and 180 ± 90 nm after subsequent crosslinking reaction at an increasing time: 3 and 5 h, respectively. The peak force quantitative nanomechanical mapping (PF-QNM) indicated the increase of DMT modulus of obtained cross-linked fibres from 4.1 ± 0.8 GPa to 7.2 ± 0.5 GPa. Furthermore, the successful crosslinking reaction of PVP and BP solution into hydrogels was investigated in terms of examining photo-crosslinking mechanism and was confirmed by rheology, Raman, Fourier transform infrared and nuclear magnetic resonance. Finally, lysozyme was successfully encapsulated within cross-linked PVP-BP hydrogels and these were successfully electrospun into mats which were found to be as effective antibacterial agents as pure lysozyme molecules. The dissolution rate of photo cross-linked PVP mats was observed to increase in comparison to pure PVP electrospun mats which opened a potential route for their use as antibacterial, on-demand, dissolvable coatings for various biomedical applications. |
| first_indexed | 2024-12-11T08:09:34Z |
| format | Article |
| id | doaj.art-497e780ce09444659a1f148d1b58b0f9 |
| institution | Directory Open Access Journal |
| issn | 1468-6996 1878-5514 |
| language | English |
| last_indexed | 2024-12-11T08:09:34Z |
| publishDate | 2019-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Science and Technology of Advanced Materials |
| spelling | doaj.art-497e780ce09444659a1f148d1b58b0f92022-12-22T01:14:55ZengTaylor & Francis GroupScience and Technology of Advanced Materials1468-69961878-55142019-12-0120197999110.1080/14686996.2019.16677371667737UV cross-linked polyvinylpyrrolidone electrospun fibres as antibacterial surfacesBarbara M. Maciejewska0Jacek K. Wychowaniec1Marta Woźniak-Budych2Łukasz Popenda3Alicja Warowicka4Klaudia Golba5Jagoda Litowczenko6Zbigniew Fojud7Beata Wereszczyńska8Stefan Jurga9Adam Mickiewicz UniversityAdam Mickiewicz UniversityAdam Mickiewicz UniversityAdam Mickiewicz UniversityAdam Mickiewicz UniversityAdam Mickiewicz UniversityAdam Mickiewicz UniversityAdam Mickiewicz UniversityAdam Mickiewicz UniversityAdam Mickiewicz UniversityMany bacteria become progressively more resistant to antibiotics and it remains a challenging task to control their overall levels. Polymers combined with active biomolecules come to the forefront for the design of antibacterial materials that can address this encounter. In this work, we investigated the photo-crosslinking approach of UV-sensitive benzophenone molecule (BP) with polyvinylpyrrolidone (PVP) polymer within electrospun fibres. The BP and PVP solutions allowed fabricating polymer mats that were subsequently functionalised with antibacterial lysozyme. The physical properties of the crosslinked electrospun fibres were investigated by scanning electron microscopy and atomic force microscopy. The average diameter of the obtained fibres decreased from 290 ± 50 nm to 270 ± 70 nm upon the addition of the crosslinking molecules and then to 240 ± 80 nm and 180 ± 90 nm after subsequent crosslinking reaction at an increasing time: 3 and 5 h, respectively. The peak force quantitative nanomechanical mapping (PF-QNM) indicated the increase of DMT modulus of obtained cross-linked fibres from 4.1 ± 0.8 GPa to 7.2 ± 0.5 GPa. Furthermore, the successful crosslinking reaction of PVP and BP solution into hydrogels was investigated in terms of examining photo-crosslinking mechanism and was confirmed by rheology, Raman, Fourier transform infrared and nuclear magnetic resonance. Finally, lysozyme was successfully encapsulated within cross-linked PVP-BP hydrogels and these were successfully electrospun into mats which were found to be as effective antibacterial agents as pure lysozyme molecules. The dissolution rate of photo cross-linked PVP mats was observed to increase in comparison to pure PVP electrospun mats which opened a potential route for their use as antibacterial, on-demand, dissolvable coatings for various biomedical applications.http://dx.doi.org/10.1080/14686996.2019.1667737crosslinkingelectrospinningpolymer fibreslysozymeantibacterialbenzophenonepolyvinylpyrrolidone |
| spellingShingle | Barbara M. Maciejewska Jacek K. Wychowaniec Marta Woźniak-Budych Łukasz Popenda Alicja Warowicka Klaudia Golba Jagoda Litowczenko Zbigniew Fojud Beata Wereszczyńska Stefan Jurga UV cross-linked polyvinylpyrrolidone electrospun fibres as antibacterial surfaces Science and Technology of Advanced Materials crosslinking electrospinning polymer fibres lysozyme antibacterial benzophenone polyvinylpyrrolidone |
| title | UV cross-linked polyvinylpyrrolidone electrospun fibres as antibacterial surfaces |
| title_full | UV cross-linked polyvinylpyrrolidone electrospun fibres as antibacterial surfaces |
| title_fullStr | UV cross-linked polyvinylpyrrolidone electrospun fibres as antibacterial surfaces |
| title_full_unstemmed | UV cross-linked polyvinylpyrrolidone electrospun fibres as antibacterial surfaces |
| title_short | UV cross-linked polyvinylpyrrolidone electrospun fibres as antibacterial surfaces |
| title_sort | uv cross linked polyvinylpyrrolidone electrospun fibres as antibacterial surfaces |
| topic | crosslinking electrospinning polymer fibres lysozyme antibacterial benzophenone polyvinylpyrrolidone |
| url | http://dx.doi.org/10.1080/14686996.2019.1667737 |
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