Myrtenol Inhibits Biofilm Formation and Virulence in the Drug-Resistant Acinetobacter baumannii: Insights into the Molecular Mechanisms
Lei Liu,1,2,* Bin Liu,1,2,* Liang Li,1,2 Ming-Xin He,2 Xiang-Dong Zhou,1,2 Qi Li1,2 1Department of Respiratory Medicine, the First Affiliated Hospital of Hainan Medical University, Haikou, Hainan, People’s Republic of China; 2NHC Key Laboratory of Tropical Disease Control, Hainan Med...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2022-09-01
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| Series: | Infection and Drug Resistance |
| Subjects: | |
| Online Access: | https://www.dovepress.com/myrtenol-inhibits-biofilm-formation-and-virulence-in-the-drug-resistan-peer-reviewed-fulltext-article-IDR |
| _version_ | 1798037373167075328 |
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| author | Liu L Liu B Li L He MX Zhou XD Li Q |
| author_facet | Liu L Liu B Li L He MX Zhou XD Li Q |
| author_sort | Liu L |
| collection | DOAJ |
| description | Lei Liu,1,2,* Bin Liu,1,2,* Liang Li,1,2 Ming-Xin He,2 Xiang-Dong Zhou,1,2 Qi Li1,2 1Department of Respiratory Medicine, the First Affiliated Hospital of Hainan Medical University, Haikou, Hainan, People’s Republic of China; 2NHC Key Laboratory of Tropical Disease Control, Hainan Medical University, Haikou, Hainan, People’s Republic of China*These authors contributed equally to this workCorrespondence: Qi Li, Email lqlq198210@sina.comBackground: blaNDM-1-producing Acinetobacter baumannii (BP-AB) remains a critical problem in nosocomial infections, because of its resistance mediated by the biofilm formation and virulence factors. No studies have confirmed myrtenol’s efficacy in inhibiting the biofilm formation and virulence associated with biofilm of BP-AB.Methods: The tested concentrations of myrtenol were wild type (A), 50 μg/mL (B), 100 μg/mL (C), 200 μg/mL (D), 250 μg/mL (E), and 300 μg/mL (F).Results: The BP-AB biofilm inhibition was significantly higher in the D, E, and F groups than in the A, B, and C groups. Myrtenol significantly reduced the air-liquid interface ring formation in glass tubes. It also effectively inhibited the attachment of BP-AB strains on polystyrene surfaces as shown by crystal violet staining. Microscopy showed a significant reduction in biofilm formation with dispersed BP-AB strains. The confocal laser scanning microscopy analysis showed a significant reduction in the biofilm’s biomass, covered surface area, and thickness. The scanning electron microscopy analysis revealed significantly fewer BP-AB aggregates on the coverslip surface. In the CompStat analysis, the biofilm’s biomass, maximum thickness, and surface-to-volume ratio were significantly reduced. The qPCR analysis revealed a significant down-regulation of bfmR, bap, csuA/B, and ompA expression, which positively correlated with the biofilm’s biomass, maximum thickness, and surface-to-volume ratio in BP-AB strains. Myrtenol significantly improved the susceptibility of BP-AB to the antibiotics amikacin, piperacillin/tazobactam, cefoperazone/sulbactam, and ceftazidime.Conclusion: Myrtenol attenuates the BP-AB biofilm formation and virulence by suppressing the expression of bfmR, bap, csuA/B, and ompA.Keywords: Acinetobacter baumannii, myrtenol, biofilm, virulence factors, resistance |
| first_indexed | 2024-04-11T21:25:41Z |
| format | Article |
| id | doaj.art-497f2904499c456d9e96da35b2314e30 |
| institution | Directory Open Access Journal |
| issn | 1178-6973 |
| language | English |
| last_indexed | 2024-04-11T21:25:41Z |
| publishDate | 2022-09-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Infection and Drug Resistance |
| spelling | doaj.art-497f2904499c456d9e96da35b2314e302022-12-22T04:02:24ZengDove Medical PressInfection and Drug Resistance1178-69732022-09-01Volume 155137514877919Myrtenol Inhibits Biofilm Formation and Virulence in the Drug-Resistant Acinetobacter baumannii: Insights into the Molecular MechanismsLiu LLiu BLi LHe MXZhou XDLi QLei Liu,1,2,* Bin Liu,1,2,* Liang Li,1,2 Ming-Xin He,2 Xiang-Dong Zhou,1,2 Qi Li1,2 1Department of Respiratory Medicine, the First Affiliated Hospital of Hainan Medical University, Haikou, Hainan, People’s Republic of China; 2NHC Key Laboratory of Tropical Disease Control, Hainan Medical University, Haikou, Hainan, People’s Republic of China*These authors contributed equally to this workCorrespondence: Qi Li, Email lqlq198210@sina.comBackground: blaNDM-1-producing Acinetobacter baumannii (BP-AB) remains a critical problem in nosocomial infections, because of its resistance mediated by the biofilm formation and virulence factors. No studies have confirmed myrtenol’s efficacy in inhibiting the biofilm formation and virulence associated with biofilm of BP-AB.Methods: The tested concentrations of myrtenol were wild type (A), 50 μg/mL (B), 100 μg/mL (C), 200 μg/mL (D), 250 μg/mL (E), and 300 μg/mL (F).Results: The BP-AB biofilm inhibition was significantly higher in the D, E, and F groups than in the A, B, and C groups. Myrtenol significantly reduced the air-liquid interface ring formation in glass tubes. It also effectively inhibited the attachment of BP-AB strains on polystyrene surfaces as shown by crystal violet staining. Microscopy showed a significant reduction in biofilm formation with dispersed BP-AB strains. The confocal laser scanning microscopy analysis showed a significant reduction in the biofilm’s biomass, covered surface area, and thickness. The scanning electron microscopy analysis revealed significantly fewer BP-AB aggregates on the coverslip surface. In the CompStat analysis, the biofilm’s biomass, maximum thickness, and surface-to-volume ratio were significantly reduced. The qPCR analysis revealed a significant down-regulation of bfmR, bap, csuA/B, and ompA expression, which positively correlated with the biofilm’s biomass, maximum thickness, and surface-to-volume ratio in BP-AB strains. Myrtenol significantly improved the susceptibility of BP-AB to the antibiotics amikacin, piperacillin/tazobactam, cefoperazone/sulbactam, and ceftazidime.Conclusion: Myrtenol attenuates the BP-AB biofilm formation and virulence by suppressing the expression of bfmR, bap, csuA/B, and ompA.Keywords: Acinetobacter baumannii, myrtenol, biofilm, virulence factors, resistancehttps://www.dovepress.com/myrtenol-inhibits-biofilm-formation-and-virulence-in-the-drug-resistan-peer-reviewed-fulltext-article-IDRacinetobacter baumanniimyrtenolbiofilmvirulence factorsresistance |
| spellingShingle | Liu L Liu B Li L He MX Zhou XD Li Q Myrtenol Inhibits Biofilm Formation and Virulence in the Drug-Resistant Acinetobacter baumannii: Insights into the Molecular Mechanisms Infection and Drug Resistance acinetobacter baumannii myrtenol biofilm virulence factors resistance |
| title | Myrtenol Inhibits Biofilm Formation and Virulence in the Drug-Resistant Acinetobacter baumannii: Insights into the Molecular Mechanisms |
| title_full | Myrtenol Inhibits Biofilm Formation and Virulence in the Drug-Resistant Acinetobacter baumannii: Insights into the Molecular Mechanisms |
| title_fullStr | Myrtenol Inhibits Biofilm Formation and Virulence in the Drug-Resistant Acinetobacter baumannii: Insights into the Molecular Mechanisms |
| title_full_unstemmed | Myrtenol Inhibits Biofilm Formation and Virulence in the Drug-Resistant Acinetobacter baumannii: Insights into the Molecular Mechanisms |
| title_short | Myrtenol Inhibits Biofilm Formation and Virulence in the Drug-Resistant Acinetobacter baumannii: Insights into the Molecular Mechanisms |
| title_sort | myrtenol inhibits biofilm formation and virulence in the drug resistant acinetobacter baumannii insights into the molecular mechanisms |
| topic | acinetobacter baumannii myrtenol biofilm virulence factors resistance |
| url | https://www.dovepress.com/myrtenol-inhibits-biofilm-formation-and-virulence-in-the-drug-resistan-peer-reviewed-fulltext-article-IDR |
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