Characterization of <i>Brassica rapa</i> RAP2.4-Related Proteins in Stress Response and as CUL3-Dependent E3 Ligase Substrates
The turnip <i>Brassica rapa</i> has important economic value and represents a good model system to study gene function in crop plants. ERF/AP2 transcription factors are a major group of proteins that are often involved in regulating stress-responses and developmental programs. Some ERF/A...
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| Format: | Article |
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MDPI AG
2019-04-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/8/4/336 |
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| author | Sutton Mooney Raed Al-Saharin Christina M. Choi Kyle Tucker Chase Beathard Hanjo A. Hellmann |
| author_facet | Sutton Mooney Raed Al-Saharin Christina M. Choi Kyle Tucker Chase Beathard Hanjo A. Hellmann |
| author_sort | Sutton Mooney |
| collection | DOAJ |
| description | The turnip <i>Brassica rapa</i> has important economic value and represents a good model system to study gene function in crop plants. ERF/AP2 transcription factors are a major group of proteins that are often involved in regulating stress-responses and developmental programs. Some ERF/AP2 proteins are targets of CULLIN3-based E3 ligases that use BTB/POZ-MATH proteins as substrate receptors. These receptors bind the transcription factor and facilitate their ubiquitylation and subsequent degradation via the 26S proteasome. Here, we show tissue and stress-dependent expression patterns for three <i>Brassica rapa</i> ERF/AP2 proteins that are closely related to <i>Arabidopsis thaliana</i> AtRAP2.4. Cloning of the <i>Brassica</i> genes showed that the corresponding proteins can assemble with a BPM protein and CULLIN3, and that they are instable in a 26S proteasome dependent manner. This work demonstrates the conserved nature of the ERF/AP2-CULLIN3-based E3 ligase interplay, and represents a first step to analyze their function in a commercially relevant crop plant. |
| first_indexed | 2024-03-12T06:56:59Z |
| format | Article |
| id | doaj.art-49815782bca848278271ecfee19f2e16 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T06:56:59Z |
| publishDate | 2019-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-49815782bca848278271ecfee19f2e162023-09-02T23:58:17ZengMDPI AGCells2073-44092019-04-018433610.3390/cells8040336cells8040336Characterization of <i>Brassica rapa</i> RAP2.4-Related Proteins in Stress Response and as CUL3-Dependent E3 Ligase SubstratesSutton Mooney0Raed Al-Saharin1Christina M. Choi2Kyle Tucker3Chase Beathard4Hanjo A. Hellmann5School of Biological Sciences, Washington State University, Pullman, WA 99164, USASchool of Biological Sciences, Washington State University, Pullman, WA 99164, USASchool of Biological Sciences, Washington State University, Pullman, WA 99164, USASchool of Biological Sciences, Washington State University, Pullman, WA 99164, USASchool of Biological Sciences, Washington State University, Pullman, WA 99164, USASchool of Biological Sciences, Washington State University, Pullman, WA 99164, USAThe turnip <i>Brassica rapa</i> has important economic value and represents a good model system to study gene function in crop plants. ERF/AP2 transcription factors are a major group of proteins that are often involved in regulating stress-responses and developmental programs. Some ERF/AP2 proteins are targets of CULLIN3-based E3 ligases that use BTB/POZ-MATH proteins as substrate receptors. These receptors bind the transcription factor and facilitate their ubiquitylation and subsequent degradation via the 26S proteasome. Here, we show tissue and stress-dependent expression patterns for three <i>Brassica rapa</i> ERF/AP2 proteins that are closely related to <i>Arabidopsis thaliana</i> AtRAP2.4. Cloning of the <i>Brassica</i> genes showed that the corresponding proteins can assemble with a BPM protein and CULLIN3, and that they are instable in a 26S proteasome dependent manner. This work demonstrates the conserved nature of the ERF/AP2-CULLIN3-based E3 ligase interplay, and represents a first step to analyze their function in a commercially relevant crop plant.https://www.mdpi.com/2073-4409/8/4/336RAP2.4stressproteasometranscriptionPEST |
| spellingShingle | Sutton Mooney Raed Al-Saharin Christina M. Choi Kyle Tucker Chase Beathard Hanjo A. Hellmann Characterization of <i>Brassica rapa</i> RAP2.4-Related Proteins in Stress Response and as CUL3-Dependent E3 Ligase Substrates Cells RAP2.4 stress proteasome transcription PEST |
| title | Characterization of <i>Brassica rapa</i> RAP2.4-Related Proteins in Stress Response and as CUL3-Dependent E3 Ligase Substrates |
| title_full | Characterization of <i>Brassica rapa</i> RAP2.4-Related Proteins in Stress Response and as CUL3-Dependent E3 Ligase Substrates |
| title_fullStr | Characterization of <i>Brassica rapa</i> RAP2.4-Related Proteins in Stress Response and as CUL3-Dependent E3 Ligase Substrates |
| title_full_unstemmed | Characterization of <i>Brassica rapa</i> RAP2.4-Related Proteins in Stress Response and as CUL3-Dependent E3 Ligase Substrates |
| title_short | Characterization of <i>Brassica rapa</i> RAP2.4-Related Proteins in Stress Response and as CUL3-Dependent E3 Ligase Substrates |
| title_sort | characterization of i brassica rapa i rap2 4 related proteins in stress response and as cul3 dependent e3 ligase substrates |
| topic | RAP2.4 stress proteasome transcription PEST |
| url | https://www.mdpi.com/2073-4409/8/4/336 |
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