In Silico Design of Engineering Optimization via OptHandle for Effective Synthesis of Adipic Acid Precursor, α-Aminoadipate
The green biosynthesis of adipic acid, an important monomer of nylon 6,6, has become a research hotspot. α-Aminoadipate is a key intermediate in the metabolic pathway that converts <sub>L</sub>-lysine to produce adipic acid. In addition, metabolic flux analysis has become an important pa...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/9/859 |
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| author | Yang Zhang Bingqi Cai Meng Liu Keqin He Zhijin Gong Haoran Bi Kai Wang Biqiang Chen Meng Wang Haijia Su Tianwei Tan |
| author_facet | Yang Zhang Bingqi Cai Meng Liu Keqin He Zhijin Gong Haoran Bi Kai Wang Biqiang Chen Meng Wang Haijia Su Tianwei Tan |
| author_sort | Yang Zhang |
| collection | DOAJ |
| description | The green biosynthesis of adipic acid, an important monomer of nylon 6,6, has become a research hotspot. α-Aminoadipate is a key intermediate in the metabolic pathway that converts <sub>L</sub>-lysine to produce adipic acid. In addition, metabolic flux analysis has become an important part of metabolic engineering. Many metabolic optimization algorithms have been developed to predict engineering intervention strategies with the aim of improving the production of target chemicals. Here, OptHandle, a new metabolic optimization algorithm, has been developed. And, we use OptHandle to optimize the biosynthesis of α-aminoadipate. Based on the results of OptHandle, an engineered <i>Escherichia coli</i> with a 13-fold higher titer was obtained, and 1.10 ± 0.02 g/L of α-aminoadipate was produced. The efficient synthesis of α-aminoadipate lays a foundation for the green production of adipic acid. |
| first_indexed | 2024-03-10T22:46:10Z |
| format | Article |
| id | doaj.art-498339470f12483fb845339b950fb1ba |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-10T22:46:10Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-498339470f12483fb845339b950fb1ba2023-11-19T10:39:31ZengMDPI AGFermentation2311-56372023-09-019985910.3390/fermentation9090859In Silico Design of Engineering Optimization via OptHandle for Effective Synthesis of Adipic Acid Precursor, α-AminoadipateYang Zhang0Bingqi Cai1Meng Liu2Keqin He3Zhijin Gong4Haoran Bi5Kai Wang6Biqiang Chen7Meng Wang8Haijia Su9Tianwei Tan10National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, ChinaNational Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, ChinaNational Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, ChinaNational Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, ChinaNational Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, ChinaNational Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, ChinaNational Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, ChinaNational Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, ChinaNational Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, ChinaNational Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, ChinaNational Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, ChinaThe green biosynthesis of adipic acid, an important monomer of nylon 6,6, has become a research hotspot. α-Aminoadipate is a key intermediate in the metabolic pathway that converts <sub>L</sub>-lysine to produce adipic acid. In addition, metabolic flux analysis has become an important part of metabolic engineering. Many metabolic optimization algorithms have been developed to predict engineering intervention strategies with the aim of improving the production of target chemicals. Here, OptHandle, a new metabolic optimization algorithm, has been developed. And, we use OptHandle to optimize the biosynthesis of α-aminoadipate. Based on the results of OptHandle, an engineered <i>Escherichia coli</i> with a 13-fold higher titer was obtained, and 1.10 ± 0.02 g/L of α-aminoadipate was produced. The efficient synthesis of α-aminoadipate lays a foundation for the green production of adipic acid.https://www.mdpi.com/2311-5637/9/9/859adipic acidα-aminoadipateOptHandlemetabolic optimization algorithm |
| spellingShingle | Yang Zhang Bingqi Cai Meng Liu Keqin He Zhijin Gong Haoran Bi Kai Wang Biqiang Chen Meng Wang Haijia Su Tianwei Tan In Silico Design of Engineering Optimization via OptHandle for Effective Synthesis of Adipic Acid Precursor, α-Aminoadipate Fermentation adipic acid α-aminoadipate OptHandle metabolic optimization algorithm |
| title | In Silico Design of Engineering Optimization via OptHandle for Effective Synthesis of Adipic Acid Precursor, α-Aminoadipate |
| title_full | In Silico Design of Engineering Optimization via OptHandle for Effective Synthesis of Adipic Acid Precursor, α-Aminoadipate |
| title_fullStr | In Silico Design of Engineering Optimization via OptHandle for Effective Synthesis of Adipic Acid Precursor, α-Aminoadipate |
| title_full_unstemmed | In Silico Design of Engineering Optimization via OptHandle for Effective Synthesis of Adipic Acid Precursor, α-Aminoadipate |
| title_short | In Silico Design of Engineering Optimization via OptHandle for Effective Synthesis of Adipic Acid Precursor, α-Aminoadipate |
| title_sort | in silico design of engineering optimization via opthandle for effective synthesis of adipic acid precursor α aminoadipate |
| topic | adipic acid α-aminoadipate OptHandle metabolic optimization algorithm |
| url | https://www.mdpi.com/2311-5637/9/9/859 |
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