Bufalin suppresses ovarian cancer cell proliferation via EGFR pathway
Abstract. Background:. Previous studies have shown that bufalin exerts antitumor effects through various mechanisms. This study aimed to determine the antineoplastic mechanism of bufalin, an extract of traditional Chinese medicine toad venom, in ovarian cancer. Methods:. The 3-(4,5-Dimethyl-2-thiazo...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer
2022-02-01
|
| Series: | Chinese Medical Journal |
| Online Access: | http://journals.lww.com/10.1097/CM9.0000000000001879 |
| _version_ | 1818501408434946048 |
|---|---|
| author | Lei Dou Dan Zou Feiran Song Yuxin Jin Yin Li Yi Zhang Yanjie Yin Xiuyuan Hao |
| author_facet | Lei Dou Dan Zou Feiran Song Yuxin Jin Yin Li Yi Zhang Yanjie Yin Xiuyuan Hao |
| author_sort | Lei Dou |
| collection | DOAJ |
| description | Abstract. Background:. Previous studies have shown that bufalin exerts antitumor effects through various mechanisms. This study aimed to determine the antineoplastic mechanism of bufalin, an extract of traditional Chinese medicine toad venom, in ovarian cancer.
Methods:. The 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide (MTT), 5-ethynyl-2′-deoxyuridine (EdU), and colony formation assays were used to investigate the antiproliferative effect of bufalin on the ovarian cancer cell line SK-OV-3. Molecular docking was used to investigate the combination of bufalin and epidermal growth factor receptor (EGFR) protein. Western blotting was performed to detect the expression of EGFR protein and its downstream targets.
Results:. Bufalin inhibited the proliferation of SK-OV-3 cells in a dose- and time-dependent manner. Bufalin was confirmed to combine with EGFR protein using molecular docking and downregulate expression of EGFR. Bufalin inhibited phosphorylation of EGFR, protein kinase B (AKT), and extracellular signal-regulated kinase (ERK).
Conclusion:. Bufalin suppresses the proliferation of ovarian cancer cells through the EGFR/AKT/ERK signaling pathway. |
| first_indexed | 2024-12-10T20:55:46Z |
| format | Article |
| id | doaj.art-4988446036d14108ad6b251be9ef2b6c |
| institution | Directory Open Access Journal |
| issn | 0366-6999 2542-5641 |
| language | English |
| last_indexed | 2024-12-10T20:55:46Z |
| publishDate | 2022-02-01 |
| publisher | Wolters Kluwer |
| record_format | Article |
| series | Chinese Medical Journal |
| spelling | doaj.art-4988446036d14108ad6b251be9ef2b6c2022-12-22T01:33:58ZengWolters KluwerChinese Medical Journal0366-69992542-56412022-02-01135445646110.1097/CM9.0000000000001879202202200-00012Bufalin suppresses ovarian cancer cell proliferation via EGFR pathwayLei DouDan ZouFeiran SongYuxin JinYin LiYi ZhangYanjie YinXiuyuan HaoAbstract. Background:. Previous studies have shown that bufalin exerts antitumor effects through various mechanisms. This study aimed to determine the antineoplastic mechanism of bufalin, an extract of traditional Chinese medicine toad venom, in ovarian cancer. Methods:. The 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide (MTT), 5-ethynyl-2′-deoxyuridine (EdU), and colony formation assays were used to investigate the antiproliferative effect of bufalin on the ovarian cancer cell line SK-OV-3. Molecular docking was used to investigate the combination of bufalin and epidermal growth factor receptor (EGFR) protein. Western blotting was performed to detect the expression of EGFR protein and its downstream targets. Results:. Bufalin inhibited the proliferation of SK-OV-3 cells in a dose- and time-dependent manner. Bufalin was confirmed to combine with EGFR protein using molecular docking and downregulate expression of EGFR. Bufalin inhibited phosphorylation of EGFR, protein kinase B (AKT), and extracellular signal-regulated kinase (ERK). Conclusion:. Bufalin suppresses the proliferation of ovarian cancer cells through the EGFR/AKT/ERK signaling pathway.http://journals.lww.com/10.1097/CM9.0000000000001879 |
| spellingShingle | Lei Dou Dan Zou Feiran Song Yuxin Jin Yin Li Yi Zhang Yanjie Yin Xiuyuan Hao Bufalin suppresses ovarian cancer cell proliferation via EGFR pathway Chinese Medical Journal |
| title | Bufalin suppresses ovarian cancer cell proliferation via EGFR pathway |
| title_full | Bufalin suppresses ovarian cancer cell proliferation via EGFR pathway |
| title_fullStr | Bufalin suppresses ovarian cancer cell proliferation via EGFR pathway |
| title_full_unstemmed | Bufalin suppresses ovarian cancer cell proliferation via EGFR pathway |
| title_short | Bufalin suppresses ovarian cancer cell proliferation via EGFR pathway |
| title_sort | bufalin suppresses ovarian cancer cell proliferation via egfr pathway |
| url | http://journals.lww.com/10.1097/CM9.0000000000001879 |
| work_keys_str_mv | AT leidou bufalinsuppressesovariancancercellproliferationviaegfrpathway AT danzou bufalinsuppressesovariancancercellproliferationviaegfrpathway AT feiransong bufalinsuppressesovariancancercellproliferationviaegfrpathway AT yuxinjin bufalinsuppressesovariancancercellproliferationviaegfrpathway AT yinli bufalinsuppressesovariancancercellproliferationviaegfrpathway AT yizhang bufalinsuppressesovariancancercellproliferationviaegfrpathway AT yanjieyin bufalinsuppressesovariancancercellproliferationviaegfrpathway AT xiuyuanhao bufalinsuppressesovariancancercellproliferationviaegfrpathway |