iTRAQ-Based Quantitative Proteomic Analysis of the Initiation of Head Regeneration in Planarians.
The planarian Dugesia japonica has amazing ability to regenerate a head from the anterior ends of the amputated stump with maintenance of the original anterior-posterior polarity. Although planarians present an attractive system for molecular investigation of regeneration and research has focused on...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4488856?pdf=render |
| _version_ | 1817982013394649088 |
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| author | Xiaofang Geng Gaiping Wang Yanli Qin Xiayan Zang Pengfei Li Zhi Geng Deming Xue Zimei Dong Kexue Ma Guangwen Chen Cunshuan Xu |
| author_facet | Xiaofang Geng Gaiping Wang Yanli Qin Xiayan Zang Pengfei Li Zhi Geng Deming Xue Zimei Dong Kexue Ma Guangwen Chen Cunshuan Xu |
| author_sort | Xiaofang Geng |
| collection | DOAJ |
| description | The planarian Dugesia japonica has amazing ability to regenerate a head from the anterior ends of the amputated stump with maintenance of the original anterior-posterior polarity. Although planarians present an attractive system for molecular investigation of regeneration and research has focused on clarifying the molecular mechanism of regeneration initiation in planarians at transcriptional level, but the initiation mechanism of planarian head regeneration (PHR) remains unclear at the protein level. Here, a global analysis of proteome dynamics during the early stage of PHR was performed using isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomics strategy, and our data are available via ProteomeXchange with identifier PXD002100. The results showed that 162 proteins were differentially expressed at 2 h and 6 h following amputation. Furthermore, the analysis of expression patterns and functional enrichment of the differentially expressed proteins showed that proteins involved in muscle contraction, oxidation reduction and protein synthesis were up-regulated in the initiation of PHR. Moreover, ingenuity pathway analysis showed that predominant signaling pathways such as ILK, calcium, EIF2 and mTOR signaling which were associated with cell migration, cell proliferation and protein synthesis were likely to be involved in the initiation of PHR. The results for the first time demonstrated that muscle contraction and ILK signaling might played important roles in the initiation of PHR at the global protein level. The findings of this research provide a molecular basis for further unraveling the mechanism of head regeneration initiation in planarians. |
| first_indexed | 2024-04-13T23:15:01Z |
| format | Article |
| id | doaj.art-4991dc2366fe4d4f995d4173c8bfa3ca |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T23:15:01Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-4991dc2366fe4d4f995d4173c8bfa3ca2022-12-22T02:25:26ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01107e013204510.1371/journal.pone.0132045iTRAQ-Based Quantitative Proteomic Analysis of the Initiation of Head Regeneration in Planarians.Xiaofang GengGaiping WangYanli QinXiayan ZangPengfei LiZhi GengDeming XueZimei DongKexue MaGuangwen ChenCunshuan XuThe planarian Dugesia japonica has amazing ability to regenerate a head from the anterior ends of the amputated stump with maintenance of the original anterior-posterior polarity. Although planarians present an attractive system for molecular investigation of regeneration and research has focused on clarifying the molecular mechanism of regeneration initiation in planarians at transcriptional level, but the initiation mechanism of planarian head regeneration (PHR) remains unclear at the protein level. Here, a global analysis of proteome dynamics during the early stage of PHR was performed using isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomics strategy, and our data are available via ProteomeXchange with identifier PXD002100. The results showed that 162 proteins were differentially expressed at 2 h and 6 h following amputation. Furthermore, the analysis of expression patterns and functional enrichment of the differentially expressed proteins showed that proteins involved in muscle contraction, oxidation reduction and protein synthesis were up-regulated in the initiation of PHR. Moreover, ingenuity pathway analysis showed that predominant signaling pathways such as ILK, calcium, EIF2 and mTOR signaling which were associated with cell migration, cell proliferation and protein synthesis were likely to be involved in the initiation of PHR. The results for the first time demonstrated that muscle contraction and ILK signaling might played important roles in the initiation of PHR at the global protein level. The findings of this research provide a molecular basis for further unraveling the mechanism of head regeneration initiation in planarians.http://europepmc.org/articles/PMC4488856?pdf=render |
| spellingShingle | Xiaofang Geng Gaiping Wang Yanli Qin Xiayan Zang Pengfei Li Zhi Geng Deming Xue Zimei Dong Kexue Ma Guangwen Chen Cunshuan Xu iTRAQ-Based Quantitative Proteomic Analysis of the Initiation of Head Regeneration in Planarians. PLoS ONE |
| title | iTRAQ-Based Quantitative Proteomic Analysis of the Initiation of Head Regeneration in Planarians. |
| title_full | iTRAQ-Based Quantitative Proteomic Analysis of the Initiation of Head Regeneration in Planarians. |
| title_fullStr | iTRAQ-Based Quantitative Proteomic Analysis of the Initiation of Head Regeneration in Planarians. |
| title_full_unstemmed | iTRAQ-Based Quantitative Proteomic Analysis of the Initiation of Head Regeneration in Planarians. |
| title_short | iTRAQ-Based Quantitative Proteomic Analysis of the Initiation of Head Regeneration in Planarians. |
| title_sort | itraq based quantitative proteomic analysis of the initiation of head regeneration in planarians |
| url | http://europepmc.org/articles/PMC4488856?pdf=render |
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