Interaction of Cationic Carbosilane Dendrimers and Their siRNA Complexes with MCF-7 Cells
The application of siRNA in gene therapy is mainly limited because of the problems with its transport into cells. Utilization of cationic dendrimers as siRNA carriers seems to be a promising solution in overcoming these issues, due to their positive charge and ability to penetrate cell membranes. Th...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-07-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/22/13/7097 |
| _version_ | 1797527985379606528 |
|---|---|
| author | Kamila Białkowska Katarzyna Miłowska Sylwia Michlewska Paulina Sokołowska Piotr Komorowski Tania Lozano-Cruz Rafael Gomez-Ramirez Francisco Javier de la Mata Maria Bryszewska |
| author_facet | Kamila Białkowska Katarzyna Miłowska Sylwia Michlewska Paulina Sokołowska Piotr Komorowski Tania Lozano-Cruz Rafael Gomez-Ramirez Francisco Javier de la Mata Maria Bryszewska |
| author_sort | Kamila Białkowska |
| collection | DOAJ |
| description | The application of siRNA in gene therapy is mainly limited because of the problems with its transport into cells. Utilization of cationic dendrimers as siRNA carriers seems to be a promising solution in overcoming these issues, due to their positive charge and ability to penetrate cell membranes. The following two types of carbosilane dendrimers were examined: CBD-1 and CBD-2. Dendrimers were complexed with pro-apoptotic siRNA (Mcl-1 and Bcl-2) and the complexes were characterized by measuring their zeta potential, circular dichroism and fluorescence of ethidium bromide associated with dendrimers. CBD-2/siRNA complexes were also examined by agarose gel electrophoresis. Both dendrimers form complexes with siRNA. Moreover, the cellular uptake and influence on the cell viability of the dendrimers and dendriplexes were evaluated using microscopic methods and XTT assay on MCF-7 cells. Microscopy showed that both dendrimers can transport siRNA into cells; however, a cytotoxicity assay showed differences in the toxicity of these dendrimers. |
| first_indexed | 2024-03-10T09:51:39Z |
| format | Article |
| id | doaj.art-4994b99565a441c9a5bbddc863079135 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T09:51:39Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-4994b99565a441c9a5bbddc8630791352023-11-22T02:40:06ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-07-012213709710.3390/ijms22137097Interaction of Cationic Carbosilane Dendrimers and Their siRNA Complexes with MCF-7 CellsKamila Białkowska0Katarzyna Miłowska1Sylwia Michlewska2Paulina Sokołowska3Piotr Komorowski4Tania Lozano-Cruz5Rafael Gomez-Ramirez6Francisco Javier de la Mata7Maria Bryszewska8Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska St., 90-236 Lodz, PolandDepartment of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska St., 90-236 Lodz, PolandLaboratory of Microscopic Imaging and Specialized Biological Techniques, Faculty of Biology and Environmental Protection, University of Lodz, Banacha12/16, 90-237 Lodz, PolandMolecular and Nanostructural Biophysics Laboratory, “Bionanopark” Ldt., 114/116 Dubois St., 93-465 Lodz, PolandMolecular and Nanostructural Biophysics Laboratory, “Bionanopark” Ldt., 114/116 Dubois St., 93-465 Lodz, PolandDepartment of Organic and Inorganic Chemistry, IQAR, University of Alcalá, 28805 Madrid, SpainDepartment of Organic and Inorganic Chemistry, IQAR, University of Alcalá, 28805 Madrid, SpainDepartment of Organic and Inorganic Chemistry, IQAR, University of Alcalá, 28805 Madrid, SpainDepartment of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska St., 90-236 Lodz, PolandThe application of siRNA in gene therapy is mainly limited because of the problems with its transport into cells. Utilization of cationic dendrimers as siRNA carriers seems to be a promising solution in overcoming these issues, due to their positive charge and ability to penetrate cell membranes. The following two types of carbosilane dendrimers were examined: CBD-1 and CBD-2. Dendrimers were complexed with pro-apoptotic siRNA (Mcl-1 and Bcl-2) and the complexes were characterized by measuring their zeta potential, circular dichroism and fluorescence of ethidium bromide associated with dendrimers. CBD-2/siRNA complexes were also examined by agarose gel electrophoresis. Both dendrimers form complexes with siRNA. Moreover, the cellular uptake and influence on the cell viability of the dendrimers and dendriplexes were evaluated using microscopic methods and XTT assay on MCF-7 cells. Microscopy showed that both dendrimers can transport siRNA into cells; however, a cytotoxicity assay showed differences in the toxicity of these dendrimers.https://www.mdpi.com/1422-0067/22/13/7097siRNAcarbosilane dendrimersdendriplexesnanocarriers |
| spellingShingle | Kamila Białkowska Katarzyna Miłowska Sylwia Michlewska Paulina Sokołowska Piotr Komorowski Tania Lozano-Cruz Rafael Gomez-Ramirez Francisco Javier de la Mata Maria Bryszewska Interaction of Cationic Carbosilane Dendrimers and Their siRNA Complexes with MCF-7 Cells International Journal of Molecular Sciences siRNA carbosilane dendrimers dendriplexes nanocarriers |
| title | Interaction of Cationic Carbosilane Dendrimers and Their siRNA Complexes with MCF-7 Cells |
| title_full | Interaction of Cationic Carbosilane Dendrimers and Their siRNA Complexes with MCF-7 Cells |
| title_fullStr | Interaction of Cationic Carbosilane Dendrimers and Their siRNA Complexes with MCF-7 Cells |
| title_full_unstemmed | Interaction of Cationic Carbosilane Dendrimers and Their siRNA Complexes with MCF-7 Cells |
| title_short | Interaction of Cationic Carbosilane Dendrimers and Their siRNA Complexes with MCF-7 Cells |
| title_sort | interaction of cationic carbosilane dendrimers and their sirna complexes with mcf 7 cells |
| topic | siRNA carbosilane dendrimers dendriplexes nanocarriers |
| url | https://www.mdpi.com/1422-0067/22/13/7097 |
| work_keys_str_mv | AT kamilabiałkowska interactionofcationiccarbosilanedendrimersandtheirsirnacomplexeswithmcf7cells AT katarzynamiłowska interactionofcationiccarbosilanedendrimersandtheirsirnacomplexeswithmcf7cells AT sylwiamichlewska interactionofcationiccarbosilanedendrimersandtheirsirnacomplexeswithmcf7cells AT paulinasokołowska interactionofcationiccarbosilanedendrimersandtheirsirnacomplexeswithmcf7cells AT piotrkomorowski interactionofcationiccarbosilanedendrimersandtheirsirnacomplexeswithmcf7cells AT tanialozanocruz interactionofcationiccarbosilanedendrimersandtheirsirnacomplexeswithmcf7cells AT rafaelgomezramirez interactionofcationiccarbosilanedendrimersandtheirsirnacomplexeswithmcf7cells AT franciscojavierdelamata interactionofcationiccarbosilanedendrimersandtheirsirnacomplexeswithmcf7cells AT mariabryszewska interactionofcationiccarbosilanedendrimersandtheirsirnacomplexeswithmcf7cells |