Efficient, fast and low-cost strategies for DNA extraction from different nucleated sheep cells
DNA extraction is usually the first step to perform molecular studies. This process can be nonviable due to genomic DNA (gDNA) extraction commercial kits prices. Furthermore, available DNA extraction protocols generally have high specificity, limiting their use to specific sources of biological mate...
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Maringá
2022-07-01
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| Series: | Acta Scientiarum: Biological Sciences |
| Subjects: | |
| Online Access: | https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/59272 |
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| author | André Luiz da Conceição Santos Wilson Romão Toledo da Silva Heder Nunes Ferreira Antonio Matos Fraga Junior Marcus Vinicius de Aragão Batista Jonatan Mikhail Del Solar Velarde |
| author_facet | André Luiz da Conceição Santos Wilson Romão Toledo da Silva Heder Nunes Ferreira Antonio Matos Fraga Junior Marcus Vinicius de Aragão Batista Jonatan Mikhail Del Solar Velarde |
| author_sort | André Luiz da Conceição Santos |
| collection | DOAJ |
| description | DNA extraction is usually the first step to perform molecular studies. This process can be nonviable due to genomic DNA (gDNA) extraction commercial kits prices. Furthermore, available DNA extraction protocols generally have high specificity, limiting their use to specific sources of biological material. In order to reduce costs, optimize time and laboratory logistics, besides to demonstrate a versatile protocol, the present study worked on an efficient DNA extraction protocol from somatic and non-somatic cells, using biological material from sheep as a model. For that, gDNA was extracted from whole blood, spermatozoa, and hair bulb cells, collected from three adult sheep, transported at 5ºC and stored at -20ºC until lab procedures. After extraction, gDNA concentration and purity were evaluated in a nano spectrophotometer. gDNA concentration from whole blood was greater (p < 0.05) than extracted from hair bulb cells, which in turn was superior (p < 0.05) than in spermatozoa. Also, gDNA from whole blood and, followed by, sperm showed greater (p < 0.05) purity when compared to gDNA of hair bulb cells. Adapting a gDNA extraction protocol, originally developed for bovine whole blood, enabled to obtain and isolate gDNA in different nucleated sheep cells
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| first_indexed | 2024-04-13T10:43:33Z |
| format | Article |
| id | doaj.art-499697a74b0648d7aa33712f1f934e55 |
| institution | Directory Open Access Journal |
| issn | 1679-9283 1807-863X |
| language | English |
| last_indexed | 2024-04-13T10:43:33Z |
| publishDate | 2022-07-01 |
| publisher | Universidade Estadual de Maringá |
| record_format | Article |
| series | Acta Scientiarum: Biological Sciences |
| spelling | doaj.art-499697a74b0648d7aa33712f1f934e552022-12-22T02:49:52ZengUniversidade Estadual de MaringáActa Scientiarum: Biological Sciences1679-92831807-863X2022-07-0144110.4025/actascibiolsci.v44i1.59272Efficient, fast and low-cost strategies for DNA extraction from different nucleated sheep cells André Luiz da Conceição Santos0Wilson Romão Toledo da Silva1Heder Nunes Ferreira2Antonio Matos Fraga Junior3Marcus Vinicius de Aragão Batista4Jonatan Mikhail Del Solar Velarde5Faculdade Pio Decimo / Universidade Federal de SergipeUniversidade Federal de SergipeFaculdade Pio DecimoFaculdade Pio DecimoUniversidade Federal de SergipeUniversidade Federal de SergipeDNA extraction is usually the first step to perform molecular studies. This process can be nonviable due to genomic DNA (gDNA) extraction commercial kits prices. Furthermore, available DNA extraction protocols generally have high specificity, limiting their use to specific sources of biological material. In order to reduce costs, optimize time and laboratory logistics, besides to demonstrate a versatile protocol, the present study worked on an efficient DNA extraction protocol from somatic and non-somatic cells, using biological material from sheep as a model. For that, gDNA was extracted from whole blood, spermatozoa, and hair bulb cells, collected from three adult sheep, transported at 5ºC and stored at -20ºC until lab procedures. After extraction, gDNA concentration and purity were evaluated in a nano spectrophotometer. gDNA concentration from whole blood was greater (p < 0.05) than extracted from hair bulb cells, which in turn was superior (p < 0.05) than in spermatozoa. Also, gDNA from whole blood and, followed by, sperm showed greater (p < 0.05) purity when compared to gDNA of hair bulb cells. Adapting a gDNA extraction protocol, originally developed for bovine whole blood, enabled to obtain and isolate gDNA in different nucleated sheep cells https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/59272Biotechnology; DNA extraction; hair bulb; spermatozoa; blood; genetic improvement. |
| spellingShingle | André Luiz da Conceição Santos Wilson Romão Toledo da Silva Heder Nunes Ferreira Antonio Matos Fraga Junior Marcus Vinicius de Aragão Batista Jonatan Mikhail Del Solar Velarde Efficient, fast and low-cost strategies for DNA extraction from different nucleated sheep cells Acta Scientiarum: Biological Sciences Biotechnology; DNA extraction; hair bulb; spermatozoa; blood; genetic improvement. |
| title | Efficient, fast and low-cost strategies for DNA extraction from different nucleated sheep cells |
| title_full | Efficient, fast and low-cost strategies for DNA extraction from different nucleated sheep cells |
| title_fullStr | Efficient, fast and low-cost strategies for DNA extraction from different nucleated sheep cells |
| title_full_unstemmed | Efficient, fast and low-cost strategies for DNA extraction from different nucleated sheep cells |
| title_short | Efficient, fast and low-cost strategies for DNA extraction from different nucleated sheep cells |
| title_sort | efficient fast and low cost strategies for dna extraction from different nucleated sheep cells |
| topic | Biotechnology; DNA extraction; hair bulb; spermatozoa; blood; genetic improvement. |
| url | https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/59272 |
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