Transcriptomic analysis revealing the molecular response to arsenic stress in desert Eremostachys moluccelloides Bunge
The saline, alkaline environment of arid soils is conducive to the diffusion of the metalloid arsenic (As). Desert plants in this area are of great ecological importance and practical value. However, there are few studies on the mechanism of arsenic action in desert plants. Therefore, in this study,...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-11-01
|
| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651323011120 |
| _version_ | 1797643592245706752 |
|---|---|
| author | Yongshun Zhou Fanze Meng Jinling Zhang Haonan Zhang Kai Han Changyong Liu Jianfeng Gao Fulong Chen |
| author_facet | Yongshun Zhou Fanze Meng Jinling Zhang Haonan Zhang Kai Han Changyong Liu Jianfeng Gao Fulong Chen |
| author_sort | Yongshun Zhou |
| collection | DOAJ |
| description | The saline, alkaline environment of arid soils is conducive to the diffusion of the metalloid arsenic (As). Desert plants in this area are of great ecological importance and practical value. However, there are few studies on the mechanism of arsenic action in desert plants. Therefore, in this study, Eremostachys moluccelloides Bunge was treated with different concentrations of As2O5 [As(V)] to analyze the physiological, biochemical, and transcriptomic changes of its roots and leaves and to explore the molecular mechanism of its response to As(Ⅴ) stress. The activities of catalase, superoxidase, peroxidase, and the contents of malondialdehyde and proline in roots and leaves first increased and then decreased under the As(Ⅴ) stress of different concentrations. The content of As was higher in roots than in leaves, and the As content was positively correlated with As(Ⅴ) stress concentration. In the differentially expressed gene analysis, the key enzymes of the oxidative stress response in roots and leaves were significantly enriched in the GO classification. In the KEGG pathway, genes related to the abscisic acid signal transduction pathway were co-enriched and up-regulated in roots and leaves. The related genes in the phenylpropanoid biosynthesis pathway were significantly enriched and down-regulated only in roots. In addition, the transcription factors NAC, HB-HD-ZIP, and NF-Y were up-regulated in roots and leaves. These results suggest that the higher the As(V) stress concentration, the more As is taken up by roots and leaves of E. molucelloides Bunge. In addition to causing greater oxidative damage, this may interfere with the production of secondary metabolites. Moreover, it may improve As(V) tolerance by regulating abscisic acid and transcription factors. The results will deepen our understanding of the molecular mechanism of As(Ⅴ) response in E. moluccelloides Bunge, lay the foundation for developing and applying desert plants, and provide new ideas for the phytoremediation of As pollution in arid areas. |
| first_indexed | 2024-03-11T14:17:07Z |
| format | Article |
| id | doaj.art-4996c417001c4657bc90b281dbb30ac8 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-03-11T14:17:07Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-4996c417001c4657bc90b281dbb30ac82023-11-01T04:46:05ZengElsevierEcotoxicology and Environmental Safety0147-65132023-11-01266115608Transcriptomic analysis revealing the molecular response to arsenic stress in desert Eremostachys moluccelloides BungeYongshun Zhou0Fanze Meng1Jinling Zhang2Haonan Zhang3Kai Han4Changyong Liu5Jianfeng Gao6Fulong Chen7College of Life Sciences, Shihezi University, Shihezi, Xinjiang 832000, People’s Republic of ChinaCollege of Life Sciences, Shihezi University, Shihezi, Xinjiang 832000, People’s Republic of ChinaCollege of Life Sciences, Shihezi University, Shihezi, Xinjiang 832000, People’s Republic of ChinaCollege of Life Sciences, Shihezi University, Shihezi, Xinjiang 832000, People’s Republic of ChinaCollege of Life Sciences, Shihezi University, Shihezi, Xinjiang 832000, People’s Republic of ChinaGreen Food Testing Center of the Ministry of Agriculture, Xinjiang Academy of Agricultural Reclamation Sciences, Shihezi 832003, People’s Republic of ChinaCollege of Life Sciences, Shihezi University, Shihezi, Xinjiang 832000, People’s Republic of China; Corresponding authors.College of Life Sciences, Shihezi University, Shihezi, Xinjiang 832000, People’s Republic of China; Corresponding authors.The saline, alkaline environment of arid soils is conducive to the diffusion of the metalloid arsenic (As). Desert plants in this area are of great ecological importance and practical value. However, there are few studies on the mechanism of arsenic action in desert plants. Therefore, in this study, Eremostachys moluccelloides Bunge was treated with different concentrations of As2O5 [As(V)] to analyze the physiological, biochemical, and transcriptomic changes of its roots and leaves and to explore the molecular mechanism of its response to As(Ⅴ) stress. The activities of catalase, superoxidase, peroxidase, and the contents of malondialdehyde and proline in roots and leaves first increased and then decreased under the As(Ⅴ) stress of different concentrations. The content of As was higher in roots than in leaves, and the As content was positively correlated with As(Ⅴ) stress concentration. In the differentially expressed gene analysis, the key enzymes of the oxidative stress response in roots and leaves were significantly enriched in the GO classification. In the KEGG pathway, genes related to the abscisic acid signal transduction pathway were co-enriched and up-regulated in roots and leaves. The related genes in the phenylpropanoid biosynthesis pathway were significantly enriched and down-regulated only in roots. In addition, the transcription factors NAC, HB-HD-ZIP, and NF-Y were up-regulated in roots and leaves. These results suggest that the higher the As(V) stress concentration, the more As is taken up by roots and leaves of E. molucelloides Bunge. In addition to causing greater oxidative damage, this may interfere with the production of secondary metabolites. Moreover, it may improve As(V) tolerance by regulating abscisic acid and transcription factors. The results will deepen our understanding of the molecular mechanism of As(Ⅴ) response in E. moluccelloides Bunge, lay the foundation for developing and applying desert plants, and provide new ideas for the phytoremediation of As pollution in arid areas.http://www.sciencedirect.com/science/article/pii/S0147651323011120Arid areasEremostachys moluccelloides BungeArsenic stressTranscriptomics |
| spellingShingle | Yongshun Zhou Fanze Meng Jinling Zhang Haonan Zhang Kai Han Changyong Liu Jianfeng Gao Fulong Chen Transcriptomic analysis revealing the molecular response to arsenic stress in desert Eremostachys moluccelloides Bunge Ecotoxicology and Environmental Safety Arid areas Eremostachys moluccelloides Bunge Arsenic stress Transcriptomics |
| title | Transcriptomic analysis revealing the molecular response to arsenic stress in desert Eremostachys moluccelloides Bunge |
| title_full | Transcriptomic analysis revealing the molecular response to arsenic stress in desert Eremostachys moluccelloides Bunge |
| title_fullStr | Transcriptomic analysis revealing the molecular response to arsenic stress in desert Eremostachys moluccelloides Bunge |
| title_full_unstemmed | Transcriptomic analysis revealing the molecular response to arsenic stress in desert Eremostachys moluccelloides Bunge |
| title_short | Transcriptomic analysis revealing the molecular response to arsenic stress in desert Eremostachys moluccelloides Bunge |
| title_sort | transcriptomic analysis revealing the molecular response to arsenic stress in desert eremostachys moluccelloides bunge |
| topic | Arid areas Eremostachys moluccelloides Bunge Arsenic stress Transcriptomics |
| url | http://www.sciencedirect.com/science/article/pii/S0147651323011120 |
| work_keys_str_mv | AT yongshunzhou transcriptomicanalysisrevealingthemolecularresponsetoarsenicstressindeserteremostachysmoluccelloidesbunge AT fanzemeng transcriptomicanalysisrevealingthemolecularresponsetoarsenicstressindeserteremostachysmoluccelloidesbunge AT jinlingzhang transcriptomicanalysisrevealingthemolecularresponsetoarsenicstressindeserteremostachysmoluccelloidesbunge AT haonanzhang transcriptomicanalysisrevealingthemolecularresponsetoarsenicstressindeserteremostachysmoluccelloidesbunge AT kaihan transcriptomicanalysisrevealingthemolecularresponsetoarsenicstressindeserteremostachysmoluccelloidesbunge AT changyongliu transcriptomicanalysisrevealingthemolecularresponsetoarsenicstressindeserteremostachysmoluccelloidesbunge AT jianfenggao transcriptomicanalysisrevealingthemolecularresponsetoarsenicstressindeserteremostachysmoluccelloidesbunge AT fulongchen transcriptomicanalysisrevealingthemolecularresponsetoarsenicstressindeserteremostachysmoluccelloidesbunge |