Phenotyping and genotyping studies on extended-spectrum β-lactamase-producing Escherichia coli isolates from mastitic cows on dairy farms in Egypt
Background and Aim: Extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae have become a serious public health hazard worldwide. This importance is derived from the increase of new variants, particularly blaTEM, blaSHV, and blaCTX-M genes. This study aimed to examine ESBL-producing Escher...
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| Format: | Article |
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Veterinary World
2022-04-01
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| Series: | Veterinary World |
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| Online Access: | http://www.veterinaryworld.org/Vol.15/April-2022/10.pdf |
| _version_ | 1811271833251479552 |
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| author | Shereen S. El-Mohandes Rasha H. Eid Ahmad M. Allam Hala A. A. Abou-Zeina Mohamed K. Elbayoumy |
| author_facet | Shereen S. El-Mohandes Rasha H. Eid Ahmad M. Allam Hala A. A. Abou-Zeina Mohamed K. Elbayoumy |
| author_sort | Shereen S. El-Mohandes |
| collection | DOAJ |
| description | Background and Aim: Extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae have become a serious public health hazard worldwide. This importance is derived from the increase of new variants, particularly blaTEM, blaSHV, and blaCTX-M genes. This study aimed to examine ESBL-producing Escherichia coli isolated from different governorates in Egypt from dairy cows infected with subclinical and clinical mastitis.
Materials and Methods: This study examined 207 milk samples for the resistance of isolates against 14 different antibiotics and ran serological identification of ESBL-producing E. coli isolates with complete antibiotic resistance. Genotypic and sequencing analyses of several resistance genes were conducted using a polymerase chain reaction.
Results: E. coli was identified in cases with subclinical mastitis (80.5%) and clinical mastitis (85.7%). ESBL-producing E. coli was isolated from 38.2% of subclinical mastitic milk compared to 39.3% in clinical cases, where O26:k60, O125:k70, and O25:k11 were the serotypes with complete resistance to antibiotics. ESBL-producing E. coli isolates were resistant to cefotaxime, amoxicillin, cloxacillin, oxacillin, rifampicin, and penicillin in 100% but susceptible to amoxicillin and clavulanic acid in 82.5% of the cases. Results also revealed that 51.25%, 52.5%, 66.25%, 77.5% and 60% of ESBL-producing E. coli isolates were responsive to ciprofloxacin, ofloxacin, norfloxacin, levofloxacin, and gentamycin, respectively. The detected genes were registered in GenBank as MW345819.1 and MW345820.1 for the E. coli blaTEM gene and MW295407 for the E. coli blaSHV gene.
Conclusion: This study found ESBL-producing E. coli in mastitic milk samples from Egyptian dairy farms and confirmed the occurrence and circulation of the main antibiotic genes (blaTEM and blaSHV) in the samples. Regular and thorough surveillance of ESBL-producing E. coli and subsequent preventive actions are essential for preventing the spread of these resistance genes in the future, which could pose serious and catastrophic health risks. Authorities should cling to the concept of One Health to minimize the risk of new varieties. |
| first_indexed | 2024-04-12T22:29:21Z |
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| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-04-12T22:29:21Z |
| publishDate | 2022-04-01 |
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| spelling | doaj.art-49991ae98d08465dab1d4a1734df8ee82022-12-22T03:14:02ZengVeterinary WorldVeterinary World0972-89882231-09162022-04-0115489089710.14202/vetworld.2022.890-897Phenotyping and genotyping studies on extended-spectrum β-lactamase-producing Escherichia coli isolates from mastitic cows on dairy farms in EgyptShereen S. El-Mohandes0https://orcid.org/0000-0003-0635-4654Rasha H. Eid1https://orcid.org/0000-0003-4201-4083Ahmad M. Allam2https://orcid.org/0000-0001-9776-7205Hala A. A. Abou-Zeina3https://orcid.org/0000-0001-7474-4596Mohamed K. Elbayoumy4https://orcid.org/0000-0003-2065-1911Department of Mastitis and Neonatal Diseases, Animal Reproduction Research Institute, Agriculture Research Center, Giza 12622, Egypt.Department of Mastitis and Neonatal Diseases, Animal Reproduction Research Institute, Agriculture Research Center, Giza 12622, Egypt.Department of Parasitology and Animal Diseases, Veterinary Research Institute, National Research Centre, Giza 12556, Egypt.Department of Parasitology and Animal Diseases, Veterinary Research Institute, National Research Centre, Giza 12556, Egypt.Department of Parasitology and Animal Diseases, Veterinary Research Institute, National Research Centre, Giza 12556, Egypt.Background and Aim: Extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae have become a serious public health hazard worldwide. This importance is derived from the increase of new variants, particularly blaTEM, blaSHV, and blaCTX-M genes. This study aimed to examine ESBL-producing Escherichia coli isolated from different governorates in Egypt from dairy cows infected with subclinical and clinical mastitis. Materials and Methods: This study examined 207 milk samples for the resistance of isolates against 14 different antibiotics and ran serological identification of ESBL-producing E. coli isolates with complete antibiotic resistance. Genotypic and sequencing analyses of several resistance genes were conducted using a polymerase chain reaction. Results: E. coli was identified in cases with subclinical mastitis (80.5%) and clinical mastitis (85.7%). ESBL-producing E. coli was isolated from 38.2% of subclinical mastitic milk compared to 39.3% in clinical cases, where O26:k60, O125:k70, and O25:k11 were the serotypes with complete resistance to antibiotics. ESBL-producing E. coli isolates were resistant to cefotaxime, amoxicillin, cloxacillin, oxacillin, rifampicin, and penicillin in 100% but susceptible to amoxicillin and clavulanic acid in 82.5% of the cases. Results also revealed that 51.25%, 52.5%, 66.25%, 77.5% and 60% of ESBL-producing E. coli isolates were responsive to ciprofloxacin, ofloxacin, norfloxacin, levofloxacin, and gentamycin, respectively. The detected genes were registered in GenBank as MW345819.1 and MW345820.1 for the E. coli blaTEM gene and MW295407 for the E. coli blaSHV gene. Conclusion: This study found ESBL-producing E. coli in mastitic milk samples from Egyptian dairy farms and confirmed the occurrence and circulation of the main antibiotic genes (blaTEM and blaSHV) in the samples. Regular and thorough surveillance of ESBL-producing E. coli and subsequent preventive actions are essential for preventing the spread of these resistance genes in the future, which could pose serious and catastrophic health risks. Authorities should cling to the concept of One Health to minimize the risk of new varieties.http://www.veterinaryworld.org/Vol.15/April-2022/10.pdfantibiotic resistanceescherichia coliextended-spectrum β-lactamasesmastitis |
| spellingShingle | Shereen S. El-Mohandes Rasha H. Eid Ahmad M. Allam Hala A. A. Abou-Zeina Mohamed K. Elbayoumy Phenotyping and genotyping studies on extended-spectrum β-lactamase-producing Escherichia coli isolates from mastitic cows on dairy farms in Egypt Veterinary World antibiotic resistance escherichia coli extended-spectrum β-lactamases mastitis |
| title | Phenotyping and genotyping studies on extended-spectrum β-lactamase-producing Escherichia coli isolates from mastitic cows on dairy farms in Egypt |
| title_full | Phenotyping and genotyping studies on extended-spectrum β-lactamase-producing Escherichia coli isolates from mastitic cows on dairy farms in Egypt |
| title_fullStr | Phenotyping and genotyping studies on extended-spectrum β-lactamase-producing Escherichia coli isolates from mastitic cows on dairy farms in Egypt |
| title_full_unstemmed | Phenotyping and genotyping studies on extended-spectrum β-lactamase-producing Escherichia coli isolates from mastitic cows on dairy farms in Egypt |
| title_short | Phenotyping and genotyping studies on extended-spectrum β-lactamase-producing Escherichia coli isolates from mastitic cows on dairy farms in Egypt |
| title_sort | phenotyping and genotyping studies on extended spectrum β lactamase producing escherichia coli isolates from mastitic cows on dairy farms in egypt |
| topic | antibiotic resistance escherichia coli extended-spectrum β-lactamases mastitis |
| url | http://www.veterinaryworld.org/Vol.15/April-2022/10.pdf |
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