Evaluation and comparison of antibiotic susceptibility profiles of Streptomyces spp. from clinical specimens revealed common and region-dependent resistance patterns
Abstract Notwithstanding the fact that streptomycetes are overlooked in clinical laboratories, studies describing their occurrence in disease and potential pathogenicity are emerging. Information on their species diversity in clinical specimens, aetiology and appropriate therapeutic treatment is sca...
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| Format: | Article |
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Nature Portfolio
2022-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-13094-4 |
| _version_ | 1811258043395997696 |
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| author | Lucie Kotrbová Ana Catalina Lara Erika Corretto Josef Scharfen Vít Ulmann Kateřina Petříčková Alica Chroňáková |
| author_facet | Lucie Kotrbová Ana Catalina Lara Erika Corretto Josef Scharfen Vít Ulmann Kateřina Petříčková Alica Chroňáková |
| author_sort | Lucie Kotrbová |
| collection | DOAJ |
| description | Abstract Notwithstanding the fact that streptomycetes are overlooked in clinical laboratories, studies describing their occurrence in disease and potential pathogenicity are emerging. Information on their species diversity in clinical specimens, aetiology and appropriate therapeutic treatment is scarce. We identified and evaluated the antibiotic susceptibility profile of 84 Streptomyces clinical isolates from the Czech Republic. In the absence of appropriate disk diffusion (DD) breakpoints for antibiotic susceptibility testing (AST) of Streptomyces spp., we determined DD breakpoints by correlation with the broth microdilution method and by the distribution of zone diameters among isolates. Correlation accuracy was high for 9 antibiotics, leading to the establishment of the most valid DD breakpoints for Streptomyces antibiotic susceptibility evaluation so far. Clinical strains belonged to 17 different phylotypes dominated by a cluster of strains sharing the same percentage of 16S rRNA gene sequence identity with more than one species (S. albidoflavus group, S. hydrogenans, S. resistomycificus, S. griseochromogenes; 70% of isolates). AST results showed that Streptomyces exhibited intrinsic resistance to penicillin, general susceptibility to amikacin, gentamycin, vancomycin and linezolid, and high percentage of susceptibility to tetracyclines and clarithromycin. For the remaining antibiotics, AST showed inter- and intra-species variations when compared to available literature (erythromycin, trimethoprim-sulfamethoxazole), indicating a region-dependent rather than species-specific patterns. |
| first_indexed | 2024-04-12T18:08:00Z |
| format | Article |
| id | doaj.art-4999b324c6474da7b752ba1adef0220b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-12T18:08:00Z |
| publishDate | 2022-06-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj.art-4999b324c6474da7b752ba1adef0220b2022-12-22T03:21:56ZengNature PortfolioScientific Reports2045-23222022-06-0112111210.1038/s41598-022-13094-4Evaluation and comparison of antibiotic susceptibility profiles of Streptomyces spp. from clinical specimens revealed common and region-dependent resistance patternsLucie Kotrbová0Ana Catalina Lara1Erika Corretto2Josef Scharfen3Vít Ulmann4Kateřina Petříčková5Alica Chroňáková6Institute of Soil Biology, Biology Centre Academy of Sciences of the Czech RepublicInstitute of Soil Biology, Biology Centre Academy of Sciences of the Czech RepublicInstitute of Soil Biology, Biology Centre Academy of Sciences of the Czech RepublicNational Laboratory for Pathogenic Actinomycetes, Trutnov Regional HospitalPublic Health Institute OstravaInstitute of Immunology and Microbiology, 1St Faculty of Medicine, Charles UniversityInstitute of Soil Biology, Biology Centre Academy of Sciences of the Czech RepublicAbstract Notwithstanding the fact that streptomycetes are overlooked in clinical laboratories, studies describing their occurrence in disease and potential pathogenicity are emerging. Information on their species diversity in clinical specimens, aetiology and appropriate therapeutic treatment is scarce. We identified and evaluated the antibiotic susceptibility profile of 84 Streptomyces clinical isolates from the Czech Republic. In the absence of appropriate disk diffusion (DD) breakpoints for antibiotic susceptibility testing (AST) of Streptomyces spp., we determined DD breakpoints by correlation with the broth microdilution method and by the distribution of zone diameters among isolates. Correlation accuracy was high for 9 antibiotics, leading to the establishment of the most valid DD breakpoints for Streptomyces antibiotic susceptibility evaluation so far. Clinical strains belonged to 17 different phylotypes dominated by a cluster of strains sharing the same percentage of 16S rRNA gene sequence identity with more than one species (S. albidoflavus group, S. hydrogenans, S. resistomycificus, S. griseochromogenes; 70% of isolates). AST results showed that Streptomyces exhibited intrinsic resistance to penicillin, general susceptibility to amikacin, gentamycin, vancomycin and linezolid, and high percentage of susceptibility to tetracyclines and clarithromycin. For the remaining antibiotics, AST showed inter- and intra-species variations when compared to available literature (erythromycin, trimethoprim-sulfamethoxazole), indicating a region-dependent rather than species-specific patterns.https://doi.org/10.1038/s41598-022-13094-4 |
| spellingShingle | Lucie Kotrbová Ana Catalina Lara Erika Corretto Josef Scharfen Vít Ulmann Kateřina Petříčková Alica Chroňáková Evaluation and comparison of antibiotic susceptibility profiles of Streptomyces spp. from clinical specimens revealed common and region-dependent resistance patterns Scientific Reports |
| title | Evaluation and comparison of antibiotic susceptibility profiles of Streptomyces spp. from clinical specimens revealed common and region-dependent resistance patterns |
| title_full | Evaluation and comparison of antibiotic susceptibility profiles of Streptomyces spp. from clinical specimens revealed common and region-dependent resistance patterns |
| title_fullStr | Evaluation and comparison of antibiotic susceptibility profiles of Streptomyces spp. from clinical specimens revealed common and region-dependent resistance patterns |
| title_full_unstemmed | Evaluation and comparison of antibiotic susceptibility profiles of Streptomyces spp. from clinical specimens revealed common and region-dependent resistance patterns |
| title_short | Evaluation and comparison of antibiotic susceptibility profiles of Streptomyces spp. from clinical specimens revealed common and region-dependent resistance patterns |
| title_sort | evaluation and comparison of antibiotic susceptibility profiles of streptomyces spp from clinical specimens revealed common and region dependent resistance patterns |
| url | https://doi.org/10.1038/s41598-022-13094-4 |
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