Evaluation of Cytotoxic effect of Moringa peregrina seeds on Oral Cancer, CAL 27 Cell Line and Red Blood Cells Hemolysis
Background: Moringa peregrina Forssk is a well-known plant in ethnomedicine due to its widespread uses in various diseases like cough, wound healing, rhinitis, fever, and detoxification. The plant seeds contain compounds that are cytotoxic to many cancer cells. During the therapeutic use of plants...
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| Format: | Article |
| Language: | English |
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Universidad de Antioquia
2024-03-01
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| Series: | Vitae |
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| Online Access: | https://revistas.udea.edu.co/index.php/vitae/article/view/350974 |
| _version_ | 1797275475876249600 |
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| author | Shaymaa Rukesh Maharjan |
| author_facet | Shaymaa Rukesh Maharjan |
| author_sort | Shaymaa |
| collection | DOAJ |
| description |
Background: Moringa peregrina Forssk is a well-known plant in ethnomedicine due to its widespread uses in various diseases like cough, wound healing, rhinitis, fever, and detoxification. The plant seeds
contain compounds that are cytotoxic to many cancer cells. During the therapeutic use of plants via the oral route, some compounds present in the plants may be cytotoxic to normal cell lines and red blood cells.
Objective: This study was the first report of investigation of the cytotoxic profile on oral cancer, CAL 27, cell line, and hemolytic activities on human erythrocytes of Moringa peregrina seeds ethanolic extract (MPSE).
Methods: MPSE was screened for its cytotoxic effect against oral cancer, CAL 27, cell line using 3-(4, 5-dimethylthiazol-2-yl)-2, 5,-diphenyltetrazolium bromide (MTT) assay. The toxicity of MPSE on human erythrocytes was determined by in vitro hemolytic assay.
Results: MPSE showed significant anti-proliferative activity against oral cancer, CAL 27 cell line at lower concentrations with half maximal inhibitory concentration (IC50) value of 21.03 μg/mL. At 1,000 µg/ml of MPSE, the maximum hemolysis was found to be 14.3% which is within safer limit.
Conclusions: This study revealed a potential anti-oral cancer of MPSE and provided a baseline for its potential use in oral cancer treatment with minimum hemolytic effect on human RBCs. |
| first_indexed | 2024-03-07T15:13:56Z |
| format | Article |
| id | doaj.art-499a5a8e74cd447d8ee346c663cdaed5 |
| institution | Directory Open Access Journal |
| issn | 0121-4004 2145-2660 |
| language | English |
| last_indexed | 2024-03-07T15:13:56Z |
| publishDate | 2024-03-01 |
| publisher | Universidad de Antioquia |
| record_format | Article |
| series | Vitae |
| spelling | doaj.art-499a5a8e74cd447d8ee346c663cdaed52024-03-05T17:59:56ZengUniversidad de AntioquiaVitae0121-40042145-26602024-03-0131110.17533/udea.vitae.v31n1a350974Evaluation of Cytotoxic effect of Moringa peregrina seeds on Oral Cancer, CAL 27 Cell Line and Red Blood Cells HemolysisShaymaa0Rukesh Maharjan1University of BaghdadUniversity of Karachi Background: Moringa peregrina Forssk is a well-known plant in ethnomedicine due to its widespread uses in various diseases like cough, wound healing, rhinitis, fever, and detoxification. The plant seeds contain compounds that are cytotoxic to many cancer cells. During the therapeutic use of plants via the oral route, some compounds present in the plants may be cytotoxic to normal cell lines and red blood cells. Objective: This study was the first report of investigation of the cytotoxic profile on oral cancer, CAL 27, cell line, and hemolytic activities on human erythrocytes of Moringa peregrina seeds ethanolic extract (MPSE). Methods: MPSE was screened for its cytotoxic effect against oral cancer, CAL 27, cell line using 3-(4, 5-dimethylthiazol-2-yl)-2, 5,-diphenyltetrazolium bromide (MTT) assay. The toxicity of MPSE on human erythrocytes was determined by in vitro hemolytic assay. Results: MPSE showed significant anti-proliferative activity against oral cancer, CAL 27 cell line at lower concentrations with half maximal inhibitory concentration (IC50) value of 21.03 μg/mL. At 1,000 µg/ml of MPSE, the maximum hemolysis was found to be 14.3% which is within safer limit. Conclusions: This study revealed a potential anti-oral cancer of MPSE and provided a baseline for its potential use in oral cancer treatment with minimum hemolytic effect on human RBCs.https://revistas.udea.edu.co/index.php/vitae/article/view/350974CAL 27 cell lineMoringa peregrina seed extractErythrocyte hemolysisToxicityAnticancer |
| spellingShingle | Shaymaa Rukesh Maharjan Evaluation of Cytotoxic effect of Moringa peregrina seeds on Oral Cancer, CAL 27 Cell Line and Red Blood Cells Hemolysis Vitae CAL 27 cell line Moringa peregrina seed extract Erythrocyte hemolysis Toxicity Anticancer |
| title | Evaluation of Cytotoxic effect of Moringa peregrina seeds on Oral Cancer, CAL 27 Cell Line and Red Blood Cells Hemolysis |
| title_full | Evaluation of Cytotoxic effect of Moringa peregrina seeds on Oral Cancer, CAL 27 Cell Line and Red Blood Cells Hemolysis |
| title_fullStr | Evaluation of Cytotoxic effect of Moringa peregrina seeds on Oral Cancer, CAL 27 Cell Line and Red Blood Cells Hemolysis |
| title_full_unstemmed | Evaluation of Cytotoxic effect of Moringa peregrina seeds on Oral Cancer, CAL 27 Cell Line and Red Blood Cells Hemolysis |
| title_short | Evaluation of Cytotoxic effect of Moringa peregrina seeds on Oral Cancer, CAL 27 Cell Line and Red Blood Cells Hemolysis |
| title_sort | evaluation of cytotoxic effect of moringa peregrina seeds on oral cancer cal 27 cell line and red blood cells hemolysis |
| topic | CAL 27 cell line Moringa peregrina seed extract Erythrocyte hemolysis Toxicity Anticancer |
| url | https://revistas.udea.edu.co/index.php/vitae/article/view/350974 |
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