The oncofetal gene survivin is re-expressed in osteoarthritis and is required for chondrocyte proliferation <it>in vitro</it>
<p>Abstract</p> <p>Background</p> <p>Regulation of cell death and cell division are key processes during chondrogenesis and in cartilage homeostasis and pathology. The oncogene survivin is considered to be critical for the coordination of mitosis and maintenance of cell...
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| Format: | Article |
| Language: | English |
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BMC
2011-07-01
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| Series: | BMC Musculoskeletal Disorders |
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| Online Access: | http://www.biomedcentral.com/1471-2474/12/150 |
| _version_ | 1823998234272464896 |
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| author | Baier Clemens Grifka Joachim Grässel Susanne Schaumburger Jens Balakrishnan Sanjeevi Lechler Philipp Straub Rainer H Renkawitz Tobias |
| author_facet | Baier Clemens Grifka Joachim Grässel Susanne Schaumburger Jens Balakrishnan Sanjeevi Lechler Philipp Straub Rainer H Renkawitz Tobias |
| author_sort | Baier Clemens |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Regulation of cell death and cell division are key processes during chondrogenesis and in cartilage homeostasis and pathology. The oncogene survivin is considered to be critical for the coordination of mitosis and maintenance of cell viability during embryonic development and in cancer, and is not detectable in most adult differentiated tissues and cells. We analyzed survivin expression in osteoarthritic cartilage and its function in primary human chondrocytes <it>in vitro</it>.</p> <p>Methods</p> <p>Survivin expression was analyzed by immunoblotting and quantitative real-time PCR. The localization was visualized by immunofluorescence. Survivin functions <it>in vitro </it>were investigated by transfection of a specific siRNA.</p> <p>Results</p> <p>Survivin was expressed in human osteoarthritic cartilage, but was not detectable in macroscopically and microscopically unaffected cartilage of osteoarthritic knee joints. In primary human chondrocyte cultures, survivin was localized to heterogeneous subcellular compartments. Suppression of survivin resulted in inhibition of cell cycle progression and sensitization toward apoptotic stimuli <it>in vitro</it>.</p> <p>Conclusions</p> <p>The present study indicates a role for survivin in osteoarthritic cartilage and human chondrocytes. <it>In vitro </it>experiments indicated its involvement in cellular division and viability. Learning more about the functions of survivin in chondrocyte biology might further help toward understanding and modulating the complex processes of cartilage pathology and regeneration.</p> |
| first_indexed | 2024-12-18T17:25:50Z |
| format | Article |
| id | doaj.art-499a6601a30848ce8abb828e94e03737 |
| institution | Directory Open Access Journal |
| issn | 1471-2474 |
| language | English |
| last_indexed | 2024-12-18T17:25:50Z |
| publishDate | 2011-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Musculoskeletal Disorders |
| spelling | doaj.art-499a6601a30848ce8abb828e94e037372022-12-21T20:59:40ZengBMCBMC Musculoskeletal Disorders1471-24742011-07-0112115010.1186/1471-2474-12-150The oncofetal gene survivin is re-expressed in osteoarthritis and is required for chondrocyte proliferation <it>in vitro</it>Baier ClemensGrifka JoachimGrässel SusanneSchaumburger JensBalakrishnan SanjeeviLechler PhilippStraub Rainer HRenkawitz Tobias<p>Abstract</p> <p>Background</p> <p>Regulation of cell death and cell division are key processes during chondrogenesis and in cartilage homeostasis and pathology. The oncogene survivin is considered to be critical for the coordination of mitosis and maintenance of cell viability during embryonic development and in cancer, and is not detectable in most adult differentiated tissues and cells. We analyzed survivin expression in osteoarthritic cartilage and its function in primary human chondrocytes <it>in vitro</it>.</p> <p>Methods</p> <p>Survivin expression was analyzed by immunoblotting and quantitative real-time PCR. The localization was visualized by immunofluorescence. Survivin functions <it>in vitro </it>were investigated by transfection of a specific siRNA.</p> <p>Results</p> <p>Survivin was expressed in human osteoarthritic cartilage, but was not detectable in macroscopically and microscopically unaffected cartilage of osteoarthritic knee joints. In primary human chondrocyte cultures, survivin was localized to heterogeneous subcellular compartments. Suppression of survivin resulted in inhibition of cell cycle progression and sensitization toward apoptotic stimuli <it>in vitro</it>.</p> <p>Conclusions</p> <p>The present study indicates a role for survivin in osteoarthritic cartilage and human chondrocytes. <it>In vitro </it>experiments indicated its involvement in cellular division and viability. Learning more about the functions of survivin in chondrocyte biology might further help toward understanding and modulating the complex processes of cartilage pathology and regeneration.</p>http://www.biomedcentral.com/1471-2474/12/150apoptosischondrocyteosteoarthritisproliferationsurvivin |
| spellingShingle | Baier Clemens Grifka Joachim Grässel Susanne Schaumburger Jens Balakrishnan Sanjeevi Lechler Philipp Straub Rainer H Renkawitz Tobias The oncofetal gene survivin is re-expressed in osteoarthritis and is required for chondrocyte proliferation <it>in vitro</it> BMC Musculoskeletal Disorders apoptosis chondrocyte osteoarthritis proliferation survivin |
| title | The oncofetal gene survivin is re-expressed in osteoarthritis and is required for chondrocyte proliferation <it>in vitro</it> |
| title_full | The oncofetal gene survivin is re-expressed in osteoarthritis and is required for chondrocyte proliferation <it>in vitro</it> |
| title_fullStr | The oncofetal gene survivin is re-expressed in osteoarthritis and is required for chondrocyte proliferation <it>in vitro</it> |
| title_full_unstemmed | The oncofetal gene survivin is re-expressed in osteoarthritis and is required for chondrocyte proliferation <it>in vitro</it> |
| title_short | The oncofetal gene survivin is re-expressed in osteoarthritis and is required for chondrocyte proliferation <it>in vitro</it> |
| title_sort | oncofetal gene survivin is re expressed in osteoarthritis and is required for chondrocyte proliferation it in vitro it |
| topic | apoptosis chondrocyte osteoarthritis proliferation survivin |
| url | http://www.biomedcentral.com/1471-2474/12/150 |
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