Thoroughbred mare's milk exhibits a unique and diverse free oligosaccharide profile
The Thoroughbred is among the most valuable horse breeds, and its husbandry is a major industry. Mare's milk plays a major role in the health of neonatal foals. Although the main components of mare's milk are broadly characterized, free oligosaccharides (OS), which possess various bioactiv...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-08-01
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| Series: | FEBS Open Bio |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/2211-5463.12460 |
| _version_ | 1797375102915969024 |
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| author | Sercan Karav Jaime Salcedo Steven A. Frese Daniela Barile |
| author_facet | Sercan Karav Jaime Salcedo Steven A. Frese Daniela Barile |
| author_sort | Sercan Karav |
| collection | DOAJ |
| description | The Thoroughbred is among the most valuable horse breeds, and its husbandry is a major industry. Mare's milk plays a major role in the health of neonatal foals. Although the main components of mare's milk are broadly characterized, free oligosaccharides (OS), which possess various bioactivities in many mammalian milks, have not been fully profiled in Thoroughbreds. The aim of this study was to identify and quantify OS in Thoroughbred mare's milk during the first week of lactation, when foals typically consume mare's milk exclusively. A total of 48 OS structures (including isomers and anomers), corresponding to 20 unique compositions, were identified by nano LC‐Chip QToF‐MS and confirmed by tandem mass spectrometry. Neutral OS were the most abundant glycans (58.3%), followed by acidic OS containing Neu5Ac (33.3%), a minor presence of fucosylated OS structures (6.25%) and one structure containing NeuGc (2.1%). Comparison with other well‐characterized mammalian milks revealed that mare's milk shared 8 OS structures with human, bovine, pig and goat milk (i.e., 2 sialyllactose isomers, 3 hexose, LNH, LNT, and OS with the composition 3 Hex‐1 Neu5Ac). Additionally, there were seven unique OS not previously found in other mammal milks. During the first 7 days of lactation, the percentage of neutral and fucosylated OS increased, whereas acidic OS decreased and the total OS concentration ranged from 217.8 mg·L−1 to 79.8 mg·L−1. |
| first_indexed | 2024-03-08T19:17:22Z |
| format | Article |
| id | doaj.art-499a75e1e3824249baec46a86b3de4db |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-03-08T19:17:22Z |
| publishDate | 2018-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-499a75e1e3824249baec46a86b3de4db2023-12-27T04:21:38ZengWileyFEBS Open Bio2211-54632018-08-01881219122910.1002/2211-5463.12460Thoroughbred mare's milk exhibits a unique and diverse free oligosaccharide profileSercan Karav0Jaime Salcedo1Steven A. Frese2Daniela Barile3Department of Molecular Biology and Genetics Canakkale Onsekiz Mart University Canakkale TurkeyDepartment of Food Science and Technology University of California Davis CA USAEvolve Biosystems, Inc. Davis CA USADepartment of Food Science and Technology University of California Davis CA USAThe Thoroughbred is among the most valuable horse breeds, and its husbandry is a major industry. Mare's milk plays a major role in the health of neonatal foals. Although the main components of mare's milk are broadly characterized, free oligosaccharides (OS), which possess various bioactivities in many mammalian milks, have not been fully profiled in Thoroughbreds. The aim of this study was to identify and quantify OS in Thoroughbred mare's milk during the first week of lactation, when foals typically consume mare's milk exclusively. A total of 48 OS structures (including isomers and anomers), corresponding to 20 unique compositions, were identified by nano LC‐Chip QToF‐MS and confirmed by tandem mass spectrometry. Neutral OS were the most abundant glycans (58.3%), followed by acidic OS containing Neu5Ac (33.3%), a minor presence of fucosylated OS structures (6.25%) and one structure containing NeuGc (2.1%). Comparison with other well‐characterized mammalian milks revealed that mare's milk shared 8 OS structures with human, bovine, pig and goat milk (i.e., 2 sialyllactose isomers, 3 hexose, LNH, LNT, and OS with the composition 3 Hex‐1 Neu5Ac). Additionally, there were seven unique OS not previously found in other mammal milks. During the first 7 days of lactation, the percentage of neutral and fucosylated OS increased, whereas acidic OS decreased and the total OS concentration ranged from 217.8 mg·L−1 to 79.8 mg·L−1.https://doi.org/10.1002/2211-5463.12460free oligosaccharidesMare's milknano LC‐Chip QToF‐MS |
| spellingShingle | Sercan Karav Jaime Salcedo Steven A. Frese Daniela Barile Thoroughbred mare's milk exhibits a unique and diverse free oligosaccharide profile FEBS Open Bio free oligosaccharides Mare's milk nano LC‐Chip QToF‐MS |
| title | Thoroughbred mare's milk exhibits a unique and diverse free oligosaccharide profile |
| title_full | Thoroughbred mare's milk exhibits a unique and diverse free oligosaccharide profile |
| title_fullStr | Thoroughbred mare's milk exhibits a unique and diverse free oligosaccharide profile |
| title_full_unstemmed | Thoroughbred mare's milk exhibits a unique and diverse free oligosaccharide profile |
| title_short | Thoroughbred mare's milk exhibits a unique and diverse free oligosaccharide profile |
| title_sort | thoroughbred mare s milk exhibits a unique and diverse free oligosaccharide profile |
| topic | free oligosaccharides Mare's milk nano LC‐Chip QToF‐MS |
| url | https://doi.org/10.1002/2211-5463.12460 |
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