Methanol extract of Elsholtzia fruticosa promotes 3T3-L1 preadipocyte differentiation
Elsholtzia fruticosa (EF) is present in tropical regions throughout South Asian countries as well as the Himalayas. Although it has been used as a traditional medicine to treat digestive, respiratory, and inflammatory issues, its effect on preadipocyte differentiation is unknown. In this study, we e...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Korean Society of Animal Sciences and Technology
2024-01-01
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| Series: | Journal of Animal Science and Technology |
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| Online Access: | http://www.ejast.org/archive/view_article?doi=10.5187/jast.2024.e6 |
| _version_ | 1827357246146740224 |
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| author | Deumaya Shrestha Eunbin Kim Krishna K. Shrestha Sung-Suk Suh Sung-Hak Kim Jong Bae Seo |
| author_facet | Deumaya Shrestha Eunbin Kim Krishna K. Shrestha Sung-Suk Suh Sung-Hak Kim Jong Bae Seo |
| author_sort | Deumaya Shrestha |
| collection | DOAJ |
| description | Elsholtzia fruticosa (EF) is present in tropical regions throughout South Asian countries as well as the Himalayas. Although it has been used as a traditional medicine to treat digestive, respiratory, and inflammatory issues, its effect on preadipocyte differentiation is unknown. In this study, we examined the effects of a methanol extract prepared from EF on the differentiation of 3T3-L1 preadipocytes. Cell differentiation was assessed by microscopic observation and oil-red O staining. The expression of adipogenic and lipogenic genes, including PPARγ and C/EBPα, was measured by western blot analysis and quantitative real-time polymerase chain reaction (qRT–PCR), to provide insight into adipogenesis and lipogenesis mechanisms. The results indicated that EF promotes the differentiation of 3T3-L1 preadipocytes, with elevated lipid accumulation occurring in a concentration-dependent manner without apparent cytotoxicity. EF enhances the expression of adipogenic and lipogenic genes, including PPARγ, FABP4, adiponectin, and FAS, at the mRNA and protein levels. The effect of EF was more pronounced during the early and middle stages of 3T3-L1 cell differentiation. Treatment with EF decreased C/EBP homologous protein (CHOP) mRNA and protein levels, while increasing C/EBPα and PPARγ expression. Treatment with EF resulted in the upregulation of cyclin E and CDK2 gene expression within 24 h, followed by a decrease at 48 h, demonstrating the early-stage impact of EF. A concomitant increase in cyclin-D1 levels was observed compared with untreated cells, indicating that EF modulates lipogenic and adipogenic genes through intricate mechanisms involving CHOP and cell cycle pathways. In summary, EF induces the differentiation of 3T3-L1 preadipocytes by increasing the expression of adipogenic and lipogenic genes, possibly through CHOP and cell cycle-dependent mechanisms. |
| first_indexed | 2024-03-08T05:28:50Z |
| format | Article |
| id | doaj.art-499c64ab6719469894e2a86c0760aa71 |
| institution | Directory Open Access Journal |
| issn | 2672-0191 2055-0391 |
| language | English |
| last_indexed | 2024-03-08T05:28:50Z |
| publishDate | 2024-01-01 |
| publisher | Korean Society of Animal Sciences and Technology |
| record_format | Article |
| series | Journal of Animal Science and Technology |
| spelling | doaj.art-499c64ab6719469894e2a86c0760aa712024-02-06T08:43:49ZengKorean Society of Animal Sciences and TechnologyJournal of Animal Science and Technology2672-01912055-03912024-01-0166120421810.5187/jast.2024.e6Methanol extract of Elsholtzia fruticosa promotes 3T3-L1 preadipocyte differentiationDeumaya Shrestha0Eunbin Kim1Krishna K. Shrestha2Sung-Suk Suh3Sung-Hak Kim4Jong Bae Seo5Department of Biosciences, Mokpo National University, Jeonnam 58554, KoreaDepartment of Biomedicine, Health & Life Convergence Sciences, BK21 Four, Biomedical and Healthcare Research Institute, Mokpo National University, Jeonnam 58554, KoreaEthnobotanical Society of Nepal, MJ Mart, Kathmandu 44600, NepalDepartment of Biosciences, Mokpo National University, Jeonnam 58554, KoreaDepartment of Animal Science, Chonnam National University, Gwangju 61186, KoreaDepartment of Biosciences, Mokpo National University, Jeonnam 58554, KoreaElsholtzia fruticosa (EF) is present in tropical regions throughout South Asian countries as well as the Himalayas. Although it has been used as a traditional medicine to treat digestive, respiratory, and inflammatory issues, its effect on preadipocyte differentiation is unknown. In this study, we examined the effects of a methanol extract prepared from EF on the differentiation of 3T3-L1 preadipocytes. Cell differentiation was assessed by microscopic observation and oil-red O staining. The expression of adipogenic and lipogenic genes, including PPARγ and C/EBPα, was measured by western blot analysis and quantitative real-time polymerase chain reaction (qRT–PCR), to provide insight into adipogenesis and lipogenesis mechanisms. The results indicated that EF promotes the differentiation of 3T3-L1 preadipocytes, with elevated lipid accumulation occurring in a concentration-dependent manner without apparent cytotoxicity. EF enhances the expression of adipogenic and lipogenic genes, including PPARγ, FABP4, adiponectin, and FAS, at the mRNA and protein levels. The effect of EF was more pronounced during the early and middle stages of 3T3-L1 cell differentiation. Treatment with EF decreased C/EBP homologous protein (CHOP) mRNA and protein levels, while increasing C/EBPα and PPARγ expression. Treatment with EF resulted in the upregulation of cyclin E and CDK2 gene expression within 24 h, followed by a decrease at 48 h, demonstrating the early-stage impact of EF. A concomitant increase in cyclin-D1 levels was observed compared with untreated cells, indicating that EF modulates lipogenic and adipogenic genes through intricate mechanisms involving CHOP and cell cycle pathways. In summary, EF induces the differentiation of 3T3-L1 preadipocytes by increasing the expression of adipogenic and lipogenic genes, possibly through CHOP and cell cycle-dependent mechanisms. http://www.ejast.org/archive/view_article?doi=10.5187/jast.2024.e6AdipocyteDifferentiationAdipogenesis3T3-L1Elsholtzia fruticosaLipogenesis |
| spellingShingle | Deumaya Shrestha Eunbin Kim Krishna K. Shrestha Sung-Suk Suh Sung-Hak Kim Jong Bae Seo Methanol extract of Elsholtzia fruticosa promotes 3T3-L1 preadipocyte differentiation Journal of Animal Science and Technology Adipocyte Differentiation Adipogenesis 3T3-L1 Elsholtzia fruticosa Lipogenesis |
| title | Methanol extract of Elsholtzia fruticosa promotes 3T3-L1 preadipocyte differentiation |
| title_full | Methanol extract of Elsholtzia fruticosa promotes 3T3-L1 preadipocyte differentiation |
| title_fullStr | Methanol extract of Elsholtzia fruticosa promotes 3T3-L1 preadipocyte differentiation |
| title_full_unstemmed | Methanol extract of Elsholtzia fruticosa promotes 3T3-L1 preadipocyte differentiation |
| title_short | Methanol extract of Elsholtzia fruticosa promotes 3T3-L1 preadipocyte differentiation |
| title_sort | methanol extract of elsholtzia fruticosa promotes 3t3 l1 preadipocyte differentiation |
| topic | Adipocyte Differentiation Adipogenesis 3T3-L1 Elsholtzia fruticosa Lipogenesis |
| url | http://www.ejast.org/archive/view_article?doi=10.5187/jast.2024.e6 |
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