| Summary: | Domoic acid (DA) is an amnesic shellfish poisoning toxin produced by some species of the genera <i>Pseudo-nitzschia</i> and <i>Nitzschia</i>. This toxin has harmful effects on various species, especially scallops. This study aimed to investigate the effects of DA exposure on the immune and physical responses of bay scallop, <i>Argopecten irradians</i>. Various immunological and physical parameters were assessed (acid phosphatase (ACP), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), lipid peroxide (LPO), nitric oxide (NO), and the total protein content) in the haemolymph of scallops at 3, 6, 12, 24, and 48 h post-exposure to DA at different concentrations (10, 50, and 100 ng/mL). Moreover, the expression of immune-related genes (<i>CLT-6, FREP, HSP90, MT, PGRP,</i> and <i>PrxV</i>) was assessed. The activities of ACP, ALP, and LDH and the total protein content and LPO increased upon exposure to DA at different concentrations, while NO levels were decreased. Furthermore, immune-related genes were assessed upon DA exposure. Our results showed that exposure to DA negatively impacts immune function and disrupts physiological activities in bay scallops.
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