Effects of Marine Toxin Domoic Acid on Innate Immune Responses in Bay Scallop <i>Argopecten irradians</i>
Domoic acid (DA) is an amnesic shellfish poisoning toxin produced by some species of the genera <i>Pseudo-nitzschia</i> and <i>Nitzschia</i>. This toxin has harmful effects on various species, especially scallops. This study aimed to investigate the effects of DA exposure on...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-11-01
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| Series: | Journal of Marine Science and Engineering |
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| Online Access: | https://www.mdpi.com/2077-1312/7/11/407 |
| _version_ | 1818605978562592768 |
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| author | Cheng Chi Caiyan Zhang Jiadai Liu Xiaochuan Zheng |
| author_facet | Cheng Chi Caiyan Zhang Jiadai Liu Xiaochuan Zheng |
| author_sort | Cheng Chi |
| collection | DOAJ |
| description | Domoic acid (DA) is an amnesic shellfish poisoning toxin produced by some species of the genera <i>Pseudo-nitzschia</i> and <i>Nitzschia</i>. This toxin has harmful effects on various species, especially scallops. This study aimed to investigate the effects of DA exposure on the immune and physical responses of bay scallop, <i>Argopecten irradians</i>. Various immunological and physical parameters were assessed (acid phosphatase (ACP), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), lipid peroxide (LPO), nitric oxide (NO), and the total protein content) in the haemolymph of scallops at 3, 6, 12, 24, and 48 h post-exposure to DA at different concentrations (10, 50, and 100 ng/mL). Moreover, the expression of immune-related genes (<i>CLT-6, FREP, HSP90, MT, PGRP,</i> and <i>PrxV</i>) was assessed. The activities of ACP, ALP, and LDH and the total protein content and LPO increased upon exposure to DA at different concentrations, while NO levels were decreased. Furthermore, immune-related genes were assessed upon DA exposure. Our results showed that exposure to DA negatively impacts immune function and disrupts physiological activities in bay scallops. |
| first_indexed | 2024-12-16T14:03:33Z |
| format | Article |
| id | doaj.art-499ee0e52be343499e115060de66411a |
| institution | Directory Open Access Journal |
| issn | 2077-1312 |
| language | English |
| last_indexed | 2024-12-16T14:03:33Z |
| publishDate | 2019-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Marine Science and Engineering |
| spelling | doaj.art-499ee0e52be343499e115060de66411a2022-12-21T22:28:59ZengMDPI AGJournal of Marine Science and Engineering2077-13122019-11-0171140710.3390/jmse7110407jmse7110407Effects of Marine Toxin Domoic Acid on Innate Immune Responses in Bay Scallop <i>Argopecten irradians</i>Cheng Chi0Caiyan Zhang1Jiadai Liu2Xiaochuan Zheng3Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, Jiangsu Province, ChinaKey Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, Jiangsu Province, ChinaKey Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, Jiangsu Province, ChinaKey Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, Jiangsu Province, ChinaDomoic acid (DA) is an amnesic shellfish poisoning toxin produced by some species of the genera <i>Pseudo-nitzschia</i> and <i>Nitzschia</i>. This toxin has harmful effects on various species, especially scallops. This study aimed to investigate the effects of DA exposure on the immune and physical responses of bay scallop, <i>Argopecten irradians</i>. Various immunological and physical parameters were assessed (acid phosphatase (ACP), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), lipid peroxide (LPO), nitric oxide (NO), and the total protein content) in the haemolymph of scallops at 3, 6, 12, 24, and 48 h post-exposure to DA at different concentrations (10, 50, and 100 ng/mL). Moreover, the expression of immune-related genes (<i>CLT-6, FREP, HSP90, MT, PGRP,</i> and <i>PrxV</i>) was assessed. The activities of ACP, ALP, and LDH and the total protein content and LPO increased upon exposure to DA at different concentrations, while NO levels were decreased. Furthermore, immune-related genes were assessed upon DA exposure. Our results showed that exposure to DA negatively impacts immune function and disrupts physiological activities in bay scallops.https://www.mdpi.com/2077-1312/7/11/407domoic acidbay scallop<i>argopecten irradians</i>harmful algal bloomsimmune response |
| spellingShingle | Cheng Chi Caiyan Zhang Jiadai Liu Xiaochuan Zheng Effects of Marine Toxin Domoic Acid on Innate Immune Responses in Bay Scallop <i>Argopecten irradians</i> Journal of Marine Science and Engineering domoic acid bay scallop <i>argopecten irradians</i> harmful algal blooms immune response |
| title | Effects of Marine Toxin Domoic Acid on Innate Immune Responses in Bay Scallop <i>Argopecten irradians</i> |
| title_full | Effects of Marine Toxin Domoic Acid on Innate Immune Responses in Bay Scallop <i>Argopecten irradians</i> |
| title_fullStr | Effects of Marine Toxin Domoic Acid on Innate Immune Responses in Bay Scallop <i>Argopecten irradians</i> |
| title_full_unstemmed | Effects of Marine Toxin Domoic Acid on Innate Immune Responses in Bay Scallop <i>Argopecten irradians</i> |
| title_short | Effects of Marine Toxin Domoic Acid on Innate Immune Responses in Bay Scallop <i>Argopecten irradians</i> |
| title_sort | effects of marine toxin domoic acid on innate immune responses in bay scallop i argopecten irradians i |
| topic | domoic acid bay scallop <i>argopecten irradians</i> harmful algal blooms immune response |
| url | https://www.mdpi.com/2077-1312/7/11/407 |
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