Clinical significance of serum levels of 14-3-3β protein in patients with stable chronic obstructive pulmonary disease
Abstract Nowadays, the diagnosis and treatment of COPD are often based on the results of lung function tests. Certain individuals, however, are not candidates for lung function testing due to pulmonary bullae, cardiac failure, low lung function, and other factors. Therefore, we evaluated whether ser...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-32096-4 |
| _version_ | 1827979504221421568 |
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| author | Decai Wang Lizong Rao Huiren Lei Wencui Li Qiufang Yu Wei Li Jianghong Wei Shuyun Xu Biwen Mo |
| author_facet | Decai Wang Lizong Rao Huiren Lei Wencui Li Qiufang Yu Wei Li Jianghong Wei Shuyun Xu Biwen Mo |
| author_sort | Decai Wang |
| collection | DOAJ |
| description | Abstract Nowadays, the diagnosis and treatment of COPD are often based on the results of lung function tests. Certain individuals, however, are not candidates for lung function testing due to pulmonary bullae, cardiac failure, low lung function, and other factors. Therefore, we evaluated whether serum tyrosine3-monooxygenase/tryptophan5-monooxygenase activation protein β (14-3-3β) could be a biomarker for the diagnosis of stable COPD patients. The expression of serum 14-3-3β protein was evaluated by an enzyme-linked immunosorbent assay. The association between its concentrations and clinical parameters of stable COPD patients were analyzed by correlation analysis and ROC curve. The results before propensity score matching (PSM) showed that serum 14-3-3β protein concentrations (ng/ml) in stable COPD patients were significantly higher than in healthy controls (P < 0.001). Furthermore, serum 14-3-3β protein concentrations were higher in GOLD 3&4 COPD patients compared with healthy participants, GOLD 1 and GOLD 2 COPD patients (P < 0.05), which shows that the concentration of 14-3-3β protein correlates with disease severity in stable COPD patients. After 1:1 PSM, there was also a statistically significant rise in 14–3-3 protein levels in stable COPD patients compared to healthy controls (P < 0.01). Serum 14-3-3β protein levels were positively correlated with blood neutrophil levels (P < 0.05), and negatively related to lung function parameters in stable COPD patients (P < 0.01). When the cutoff value was set at 29.53 ng/ml, the ROC curve yielded a sensitivity of 84.9% and a specificity of 68.3% for diagnosing stable COPD. The 14-3-3β protein may be a potential serum biomarker for the diagnosis of stable COPD patients, which is associated with disease severity, systemic inflammation, and small airway obstruction. |
| first_indexed | 2024-04-09T21:37:48Z |
| format | Article |
| id | doaj.art-49a0b304e7d4423e85b96611f92cad06 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T21:37:48Z |
| publishDate | 2023-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-49a0b304e7d4423e85b96611f92cad062023-03-26T11:11:01ZengNature PortfolioScientific Reports2045-23222023-03-0113111010.1038/s41598-023-32096-4Clinical significance of serum levels of 14-3-3β protein in patients with stable chronic obstructive pulmonary diseaseDecai Wang0Lizong Rao1Huiren Lei2Wencui Li3Qiufang Yu4Wei Li5Jianghong Wei6Shuyun Xu7Biwen Mo8Department of Respiratory and Critical Care Medicine, Key Laboratory of Pulmonary Diseases of Health Ministry, Key Site of National Clinical Research Center for Respiratory Disease, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyKey Laboratory of Respiratory Disease of Zhejiang Province, Department of Respiratory and Critical Care Medicine, Second Affiliated Hospital of Zhejiang University School of MedicineDepartment of Respiratory and Critical Care Medicine, Guangxi Zhuang Autonomous Region Education Department Key Laboratory of Respiratory Diseases, Guangxi Health Commission Key Laboratory of Glucose and Lipid Metabolism Disorders, Second Affiliated Hospital of Guilin Medical UniversityDepartment of Respiratory and Critical Care Medicine, Guangxi Zhuang Autonomous Region Education Department Key Laboratory of Respiratory Diseases, Guangxi Health Commission Key Laboratory of Glucose and Lipid Metabolism Disorders, Second Affiliated Hospital of Guilin Medical UniversityDepartment of Respiratory and Critical Care Medicine, Guangxi Zhuang Autonomous Region Education Department Key Laboratory of Respiratory Diseases, Guangxi Health Commission Key Laboratory of Glucose and Lipid Metabolism Disorders, Second Affiliated Hospital of Guilin Medical UniversityDepartment of Respiratory and Critical Care Medicine, Guangxi Zhuang Autonomous Region Education Department Key Laboratory of Respiratory Diseases, Guangxi Health Commission Key Laboratory of Glucose and Lipid Metabolism Disorders, Second Affiliated Hospital of Guilin Medical UniversityDepartment of Respiratory and Critical Care Medicine, Affiliated Hospital of Guilin Medical UniversityDepartment of Respiratory and Critical Care Medicine, Key Laboratory of Pulmonary Diseases of Health Ministry, Key Site of National Clinical Research Center for Respiratory Disease, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Respiratory and Critical Care Medicine, Guangxi Zhuang Autonomous Region Education Department Key Laboratory of Respiratory Diseases, Guangxi Health Commission Key Laboratory of Glucose and Lipid Metabolism Disorders, Second Affiliated Hospital of Guilin Medical UniversityAbstract Nowadays, the diagnosis and treatment of COPD are often based on the results of lung function tests. Certain individuals, however, are not candidates for lung function testing due to pulmonary bullae, cardiac failure, low lung function, and other factors. Therefore, we evaluated whether serum tyrosine3-monooxygenase/tryptophan5-monooxygenase activation protein β (14-3-3β) could be a biomarker for the diagnosis of stable COPD patients. The expression of serum 14-3-3β protein was evaluated by an enzyme-linked immunosorbent assay. The association between its concentrations and clinical parameters of stable COPD patients were analyzed by correlation analysis and ROC curve. The results before propensity score matching (PSM) showed that serum 14-3-3β protein concentrations (ng/ml) in stable COPD patients were significantly higher than in healthy controls (P < 0.001). Furthermore, serum 14-3-3β protein concentrations were higher in GOLD 3&4 COPD patients compared with healthy participants, GOLD 1 and GOLD 2 COPD patients (P < 0.05), which shows that the concentration of 14-3-3β protein correlates with disease severity in stable COPD patients. After 1:1 PSM, there was also a statistically significant rise in 14–3-3 protein levels in stable COPD patients compared to healthy controls (P < 0.01). Serum 14-3-3β protein levels were positively correlated with blood neutrophil levels (P < 0.05), and negatively related to lung function parameters in stable COPD patients (P < 0.01). When the cutoff value was set at 29.53 ng/ml, the ROC curve yielded a sensitivity of 84.9% and a specificity of 68.3% for diagnosing stable COPD. The 14-3-3β protein may be a potential serum biomarker for the diagnosis of stable COPD patients, which is associated with disease severity, systemic inflammation, and small airway obstruction.https://doi.org/10.1038/s41598-023-32096-4 |
| spellingShingle | Decai Wang Lizong Rao Huiren Lei Wencui Li Qiufang Yu Wei Li Jianghong Wei Shuyun Xu Biwen Mo Clinical significance of serum levels of 14-3-3β protein in patients with stable chronic obstructive pulmonary disease Scientific Reports |
| title | Clinical significance of serum levels of 14-3-3β protein in patients with stable chronic obstructive pulmonary disease |
| title_full | Clinical significance of serum levels of 14-3-3β protein in patients with stable chronic obstructive pulmonary disease |
| title_fullStr | Clinical significance of serum levels of 14-3-3β protein in patients with stable chronic obstructive pulmonary disease |
| title_full_unstemmed | Clinical significance of serum levels of 14-3-3β protein in patients with stable chronic obstructive pulmonary disease |
| title_short | Clinical significance of serum levels of 14-3-3β protein in patients with stable chronic obstructive pulmonary disease |
| title_sort | clinical significance of serum levels of 14 3 3β protein in patients with stable chronic obstructive pulmonary disease |
| url | https://doi.org/10.1038/s41598-023-32096-4 |
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