LncRNA LINC00483 promotes gastric cancer development through regulating MAPK1 expression by sponging miR-490-3p
Abstract Background Previous studies have shown that long noncoding RNA (lncRNA) LINC00483 was aberrantly expressed in human cancers, including gastric cancer. However, the regulatory mechanism of this lncRNA in gastric cancer remains largely unknown. The present study aimed to investigate the effec...
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| Format: | Article |
| Language: | English |
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BMC
2020-04-01
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| Series: | Biological Research |
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| Online Access: | http://link.springer.com/article/10.1186/s40659-020-00283-6 |
| _version_ | 1818410418324897792 |
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| author | Min Luo Chengbai Liang |
| author_facet | Min Luo Chengbai Liang |
| author_sort | Min Luo |
| collection | DOAJ |
| description | Abstract Background Previous studies have shown that long noncoding RNA (lncRNA) LINC00483 was aberrantly expressed in human cancers, including gastric cancer. However, the regulatory mechanism of this lncRNA in gastric cancer remains largely unknown. The present study aimed to investigate the effect of LINC00483 on gastric cancer development and explore the potential regulatory network of LINC00483/microRNA (miR)-490-3p/mitogen-activated protein kinase 1 (MAPK1). Methods Thirty patients with gastric cancer were recruited for tissues collection. The expression levels of LINC00483, miR-490-3p and MAPK1 were detected by quantitative real-time polymerase chain reaction or western blot. Cell viability, apoptosis, migration and invasion were determined by MTT, flow cytometry, transwell assays and western blot, respectively. The target association between miR-490-3p and LINC00483 or MAPK1 was confirmed by luciferase reporter assay. Xenograft model was established to assess the function of LINC00483 in vivo. Results LINC00483 and MAPK1 levels were increased in gastric cancer tissues and cells. Knockdown of LINC00483 or MAPK1 inhibited cells viability, migration and invasion but promoted apoptosis in gastric cancer cells. Moreover, MAPK1 overexpression attenuated the effect of LINC00483 knockdown on gastric cancer development. LINC00483 could increase MAPK1 expression by competitively sponging miR-490-3p. miR-490-3p overexpression suppressed gastric cancer development, which was abated by introduction of LINC00483. Besides, inhibition of LINC00483 decreased xenograft tumor growth by regulating miR-490-3p/MAPK1 axis. Conclusion Knockdown of LINC00483 inhibited gastric cancer development in vitro and in vivo by increasing miR-490-3p and decreasing MAPK1, elucidating a novel mechanism for understanding the development of gastric cancer. |
| first_indexed | 2024-12-14T10:15:12Z |
| format | Article |
| id | doaj.art-49a1fd46a1284f1d8aa1d9d4d8c52661 |
| institution | Directory Open Access Journal |
| issn | 0717-6287 |
| language | English |
| last_indexed | 2024-12-14T10:15:12Z |
| publishDate | 2020-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Research |
| spelling | doaj.art-49a1fd46a1284f1d8aa1d9d4d8c526612022-12-21T23:06:52ZengBMCBiological Research0717-62872020-04-0153111210.1186/s40659-020-00283-6LncRNA LINC00483 promotes gastric cancer development through regulating MAPK1 expression by sponging miR-490-3pMin Luo0Chengbai Liang1Departments of Gastroenterology, The Second Xiangya Hospital of Central South UniversityDepartments of Gastroenterology, The Second Xiangya Hospital of Central South UniversityAbstract Background Previous studies have shown that long noncoding RNA (lncRNA) LINC00483 was aberrantly expressed in human cancers, including gastric cancer. However, the regulatory mechanism of this lncRNA in gastric cancer remains largely unknown. The present study aimed to investigate the effect of LINC00483 on gastric cancer development and explore the potential regulatory network of LINC00483/microRNA (miR)-490-3p/mitogen-activated protein kinase 1 (MAPK1). Methods Thirty patients with gastric cancer were recruited for tissues collection. The expression levels of LINC00483, miR-490-3p and MAPK1 were detected by quantitative real-time polymerase chain reaction or western blot. Cell viability, apoptosis, migration and invasion were determined by MTT, flow cytometry, transwell assays and western blot, respectively. The target association between miR-490-3p and LINC00483 or MAPK1 was confirmed by luciferase reporter assay. Xenograft model was established to assess the function of LINC00483 in vivo. Results LINC00483 and MAPK1 levels were increased in gastric cancer tissues and cells. Knockdown of LINC00483 or MAPK1 inhibited cells viability, migration and invasion but promoted apoptosis in gastric cancer cells. Moreover, MAPK1 overexpression attenuated the effect of LINC00483 knockdown on gastric cancer development. LINC00483 could increase MAPK1 expression by competitively sponging miR-490-3p. miR-490-3p overexpression suppressed gastric cancer development, which was abated by introduction of LINC00483. Besides, inhibition of LINC00483 decreased xenograft tumor growth by regulating miR-490-3p/MAPK1 axis. Conclusion Knockdown of LINC00483 inhibited gastric cancer development in vitro and in vivo by increasing miR-490-3p and decreasing MAPK1, elucidating a novel mechanism for understanding the development of gastric cancer.http://link.springer.com/article/10.1186/s40659-020-00283-6Gastric cancerLINC00483miR-490-3pMAPK1 |
| spellingShingle | Min Luo Chengbai Liang LncRNA LINC00483 promotes gastric cancer development through regulating MAPK1 expression by sponging miR-490-3p Biological Research Gastric cancer LINC00483 miR-490-3p MAPK1 |
| title | LncRNA LINC00483 promotes gastric cancer development through regulating MAPK1 expression by sponging miR-490-3p |
| title_full | LncRNA LINC00483 promotes gastric cancer development through regulating MAPK1 expression by sponging miR-490-3p |
| title_fullStr | LncRNA LINC00483 promotes gastric cancer development through regulating MAPK1 expression by sponging miR-490-3p |
| title_full_unstemmed | LncRNA LINC00483 promotes gastric cancer development through regulating MAPK1 expression by sponging miR-490-3p |
| title_short | LncRNA LINC00483 promotes gastric cancer development through regulating MAPK1 expression by sponging miR-490-3p |
| title_sort | lncrna linc00483 promotes gastric cancer development through regulating mapk1 expression by sponging mir 490 3p |
| topic | Gastric cancer LINC00483 miR-490-3p MAPK1 |
| url | http://link.springer.com/article/10.1186/s40659-020-00283-6 |
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