Acute and sub-acute (30-day) toxicity studies of Aegialitis rotundifolia Roxb., leaves extract in Wistar rats: safety assessment of a rare mangrove traditionally utilized as pain antidote

Abstract Background Aegialitis rotundifolia Roxb., (Plumbaginaceae) is a small mangrove tree or shrub traditionally used by the local healers of mangroves for curing pain and inflammation. However, there are no scientific reports of its toxicological properties which would guarantee the safety of it...

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Main Authors: Debjit Ghosh, Sumanta Mondal, K. Ramakrishna
Format: Article
Language:English
Published: SpringerOpen 2019-03-01
Series:Clinical Phytoscience
Subjects:
Online Access:http://link.springer.com/article/10.1186/s40816-019-0106-2
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author Debjit Ghosh
Sumanta Mondal
K. Ramakrishna
author_facet Debjit Ghosh
Sumanta Mondal
K. Ramakrishna
author_sort Debjit Ghosh
collection DOAJ
description Abstract Background Aegialitis rotundifolia Roxb., (Plumbaginaceae) is a small mangrove tree or shrub traditionally used by the local healers of mangroves for curing pain and inflammation. However, there are no scientific reports of its toxicological properties which would guarantee the safety of its folkloric usage as a potent pain reliever. Therefore, the present study deals in acute and sub-acute (30-day) toxicity studies of Aegialitis rotundifolia ethanolic leaves extract (ARELE) on male and female Wistar rats administered through oral gavage. Methods Acute toxicity study was conducted at a single oral dose of 1000, 1500, and 2000 mg/kg, body weight (b.wt.) for 14 days with a special emphasis on the first four hours after drug administration to find out any mortality and morbidity. In sub-acute toxicity, the extract at the doses of 125, 250 and 500 mg/kg, b.wt., was administered orally for 30 days. Important parameters such as general behaviour, body and organ weight, urinalysis, haematological and biochemical profile, organ macroscopy and microscopy were conducted. Organ sample of liver, kidney, pancreas, heart, lungs, and stomach were taken from both male and female rats, whereas the sample of testis and ovary was taken from male and female rats respectively for organ necropsy and histopathological studies. Neurobehavioral toxicity was conducted by performing a functional observational battery (FOB) and locomotor activity on the initial and final week of the 30-day study period. Results No mortality or any major signs of morbidity was recorded for acute toxicity except for the limit dose (2000 mg/kg, b.wt.) which produced a slight short-term sedative effect. In sub-acute toxicity, no major alteration was observed in the evaluated parameters. However, few minor changes were recorded for high dose (500 mg/kg, b.wt.) group. Conclusion The results of the present study showed that oral administration of Aegialitis rotundifolia ethanolic leaves extract did not produce any severe toxic effects in both acute and sub-acute studies in Wistar rats. Therefore, usage of an appropriate amount of Aegialitis rotundifolia leaves extract preferably at low doses for its traditional use should be considered safe.
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spelling doaj.art-49a3cb941ffb490ab7386ba1f4376d652022-12-22T01:06:39ZengSpringerOpenClinical Phytoscience2199-11972019-03-015111610.1186/s40816-019-0106-2Acute and sub-acute (30-day) toxicity studies of Aegialitis rotundifolia Roxb., leaves extract in Wistar rats: safety assessment of a rare mangrove traditionally utilized as pain antidoteDebjit Ghosh0Sumanta Mondal1K. Ramakrishna2Department of Chemistry, GITAM Institute of Science, GITAM (Deemed to be University)GITAM Institute of Pharmacy, GITAM (Deemed to be University)Department of Chemistry, GITAM Institute of Science, GITAM (Deemed to be University)Abstract Background Aegialitis rotundifolia Roxb., (Plumbaginaceae) is a small mangrove tree or shrub traditionally used by the local healers of mangroves for curing pain and inflammation. However, there are no scientific reports of its toxicological properties which would guarantee the safety of its folkloric usage as a potent pain reliever. Therefore, the present study deals in acute and sub-acute (30-day) toxicity studies of Aegialitis rotundifolia ethanolic leaves extract (ARELE) on male and female Wistar rats administered through oral gavage. Methods Acute toxicity study was conducted at a single oral dose of 1000, 1500, and 2000 mg/kg, body weight (b.wt.) for 14 days with a special emphasis on the first four hours after drug administration to find out any mortality and morbidity. In sub-acute toxicity, the extract at the doses of 125, 250 and 500 mg/kg, b.wt., was administered orally for 30 days. Important parameters such as general behaviour, body and organ weight, urinalysis, haematological and biochemical profile, organ macroscopy and microscopy were conducted. Organ sample of liver, kidney, pancreas, heart, lungs, and stomach were taken from both male and female rats, whereas the sample of testis and ovary was taken from male and female rats respectively for organ necropsy and histopathological studies. Neurobehavioral toxicity was conducted by performing a functional observational battery (FOB) and locomotor activity on the initial and final week of the 30-day study period. Results No mortality or any major signs of morbidity was recorded for acute toxicity except for the limit dose (2000 mg/kg, b.wt.) which produced a slight short-term sedative effect. In sub-acute toxicity, no major alteration was observed in the evaluated parameters. However, few minor changes were recorded for high dose (500 mg/kg, b.wt.) group. Conclusion The results of the present study showed that oral administration of Aegialitis rotundifolia ethanolic leaves extract did not produce any severe toxic effects in both acute and sub-acute studies in Wistar rats. Therefore, usage of an appropriate amount of Aegialitis rotundifolia leaves extract preferably at low doses for its traditional use should be considered safe.http://link.springer.com/article/10.1186/s40816-019-0106-2Aegialitis rotundifoliaMangroveAcute toxicitySub-acute toxicityNeurotoxicityLocomotor
spellingShingle Debjit Ghosh
Sumanta Mondal
K. Ramakrishna
Acute and sub-acute (30-day) toxicity studies of Aegialitis rotundifolia Roxb., leaves extract in Wistar rats: safety assessment of a rare mangrove traditionally utilized as pain antidote
Clinical Phytoscience
Aegialitis rotundifolia
Mangrove
Acute toxicity
Sub-acute toxicity
Neurotoxicity
Locomotor
title Acute and sub-acute (30-day) toxicity studies of Aegialitis rotundifolia Roxb., leaves extract in Wistar rats: safety assessment of a rare mangrove traditionally utilized as pain antidote
title_full Acute and sub-acute (30-day) toxicity studies of Aegialitis rotundifolia Roxb., leaves extract in Wistar rats: safety assessment of a rare mangrove traditionally utilized as pain antidote
title_fullStr Acute and sub-acute (30-day) toxicity studies of Aegialitis rotundifolia Roxb., leaves extract in Wistar rats: safety assessment of a rare mangrove traditionally utilized as pain antidote
title_full_unstemmed Acute and sub-acute (30-day) toxicity studies of Aegialitis rotundifolia Roxb., leaves extract in Wistar rats: safety assessment of a rare mangrove traditionally utilized as pain antidote
title_short Acute and sub-acute (30-day) toxicity studies of Aegialitis rotundifolia Roxb., leaves extract in Wistar rats: safety assessment of a rare mangrove traditionally utilized as pain antidote
title_sort acute and sub acute 30 day toxicity studies of aegialitis rotundifolia roxb leaves extract in wistar rats safety assessment of a rare mangrove traditionally utilized as pain antidote
topic Aegialitis rotundifolia
Mangrove
Acute toxicity
Sub-acute toxicity
Neurotoxicity
Locomotor
url http://link.springer.com/article/10.1186/s40816-019-0106-2
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