Genome-wide identification and expression analysis of the cucumber PYL gene family
Abscisic acid (ABA) is a very important hormone in plants. It regulates growth and development of plants and plays an important role in biotic and abiotic stresses. The Pyrabactin resistance 1-like (PYR/PYL) proteins play a central role in ABA signal transduction pathways. The working system of PYL...
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PeerJ Inc.
2022-01-01
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| author | Zeyu Zhang Shilei Luo Zeci Liu Zilong Wan Xueqin Gao Yali Qiao Jihua Yu Guobin Zhang |
| author_facet | Zeyu Zhang Shilei Luo Zeci Liu Zilong Wan Xueqin Gao Yali Qiao Jihua Yu Guobin Zhang |
| author_sort | Zeyu Zhang |
| collection | DOAJ |
| description | Abscisic acid (ABA) is a very important hormone in plants. It regulates growth and development of plants and plays an important role in biotic and abiotic stresses. The Pyrabactin resistance 1-like (PYR/PYL) proteins play a central role in ABA signal transduction pathways. The working system of PYL genes in cucumber, an important economical vegetable (Cucumis sativus L.), has not been fully studied yet. Through bioinformatics, a total of 14 individual PYL genes were identified in Chinese long ‘9930’ cucumber. Fourteen PYL genes were distributed on six chromosomes of cucumber, and their encoded proteins predicted to be distributed in cytoplasm and nucleus. Based on the phylogenetic analysis, the PYL genes of cucumber, Arabidopsis, rice, apple, Brachypodium distachyon and soybeancould be classified into three groups. Genetic structures and conserved domains analysis revealed that CsPYL genes in the same group have similar exons and conserved domains. By predicting cis-elements in the promoters, we found that all CsPYL members contained hormone and stress-related elements. Additionally, the expression patterns of CsPYL genes were specific in tissues. Finally, we further examined the expression of 14 CsPYL genes under ABA, PEG, salt stress. The qRT-PCR results showed that most PYL gene expression levels were up-regulated. Furthermore, with different treatments about 3h, the relative expression of PYL8 was up-regulated and more than 20 times higher than 0h. It indicated that this gene may play an important role in abiotic stress. |
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| language | English |
| last_indexed | 2024-03-09T08:23:31Z |
| publishDate | 2022-01-01 |
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| spelling | doaj.art-49a854a1bf784eae8ce814ff5decb2642023-12-02T21:37:45ZengPeerJ Inc.PeerJ2167-83592022-01-0110e1278610.7717/peerj.12786Genome-wide identification and expression analysis of the cucumber PYL gene familyZeyu Zhang0Shilei Luo1Zeci Liu2Zilong Wan3Xueqin Gao4Yali Qiao5Jihua Yu6Guobin Zhang7State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, ChinaState Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, ChinaState Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, ChinaState Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, ChinaState Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, ChinaState Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, ChinaState Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, ChinaState Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, ChinaAbscisic acid (ABA) is a very important hormone in plants. It regulates growth and development of plants and plays an important role in biotic and abiotic stresses. The Pyrabactin resistance 1-like (PYR/PYL) proteins play a central role in ABA signal transduction pathways. The working system of PYL genes in cucumber, an important economical vegetable (Cucumis sativus L.), has not been fully studied yet. Through bioinformatics, a total of 14 individual PYL genes were identified in Chinese long ‘9930’ cucumber. Fourteen PYL genes were distributed on six chromosomes of cucumber, and their encoded proteins predicted to be distributed in cytoplasm and nucleus. Based on the phylogenetic analysis, the PYL genes of cucumber, Arabidopsis, rice, apple, Brachypodium distachyon and soybeancould be classified into three groups. Genetic structures and conserved domains analysis revealed that CsPYL genes in the same group have similar exons and conserved domains. By predicting cis-elements in the promoters, we found that all CsPYL members contained hormone and stress-related elements. Additionally, the expression patterns of CsPYL genes were specific in tissues. Finally, we further examined the expression of 14 CsPYL genes under ABA, PEG, salt stress. The qRT-PCR results showed that most PYL gene expression levels were up-regulated. Furthermore, with different treatments about 3h, the relative expression of PYL8 was up-regulated and more than 20 times higher than 0h. It indicated that this gene may play an important role in abiotic stress.https://peerj.com/articles/12786.pdfAbscisic acidCucumberPYL geneAbiotic stress |
| spellingShingle | Zeyu Zhang Shilei Luo Zeci Liu Zilong Wan Xueqin Gao Yali Qiao Jihua Yu Guobin Zhang Genome-wide identification and expression analysis of the cucumber PYL gene family PeerJ Abscisic acid Cucumber PYL gene Abiotic stress |
| title | Genome-wide identification and expression analysis of the cucumber PYL gene family |
| title_full | Genome-wide identification and expression analysis of the cucumber PYL gene family |
| title_fullStr | Genome-wide identification and expression analysis of the cucumber PYL gene family |
| title_full_unstemmed | Genome-wide identification and expression analysis of the cucumber PYL gene family |
| title_short | Genome-wide identification and expression analysis of the cucumber PYL gene family |
| title_sort | genome wide identification and expression analysis of the cucumber pyl gene family |
| topic | Abscisic acid Cucumber PYL gene Abiotic stress |
| url | https://peerj.com/articles/12786.pdf |
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