Angiotensin Converting Enzyme (ACE) Inhibitory Activity of Peptide Fraction of Germinated Pigeon Pea (<i>Cajanus cajan</i> (L.) Millsp.)
During the germination process, seeds can release various types of peptides due to the degradation of storage proteins. Some of these peptides can have biological activity (bioactive peptides). The objective of this study was to determine the ACE inhibitory activity of germinated pigeon pea peptide...
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| Format: | Article |
| Language: | English |
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Department of Chemistry, Universitas Gadjah Mada
2019-08-01
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| Series: | Indonesian Journal of Chemistry |
| Subjects: | |
| Online Access: | https://jurnal.ugm.ac.id/ijc/article/view/37513 |
| _version_ | 1818425859772514304 |
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| author | Ketut Ratnayani I Ketut Suter Nyoman Semadi Antara I Nengah Kencana Putra |
| author_facet | Ketut Ratnayani I Ketut Suter Nyoman Semadi Antara I Nengah Kencana Putra |
| author_sort | Ketut Ratnayani |
| collection | DOAJ |
| description | During the germination process, seeds can release various types of peptides due to the degradation of storage proteins. Some of these peptides can have biological activity (bioactive peptides). The objective of this study was to determine the ACE inhibitory activity of germinated pigeon pea peptide extract at various germination times and to carry out the fractionation to the extract to get the most active peptide fraction. The results showed that the highest activity of peptide extract was found on the 4th-day germination of pigeon pea with an IC50 value of 63.46 μg/mL. The peptide extract was further fractionated by centrifugal ultrafiltration method and it was found that the peptide fraction < 3 kDa had the highest ACE inhibitory activity with an IC50 value of 57.79 μg/mL. The result of identification with the LCMS method to the fraction was able to detect 4 types of the peptide with a molecular weight of 230.304, 294.303, 441.436, and 570.591 Da. These results suggested that the peptide fraction of germinated pigeon pea has the potency as an ACE inhibitory nutraceutical. |
| first_indexed | 2024-12-14T14:20:38Z |
| format | Article |
| id | doaj.art-49ae70dc05a94430a6e69095701b7d0e |
| institution | Directory Open Access Journal |
| issn | 1411-9420 2460-1578 |
| language | English |
| last_indexed | 2024-12-14T14:20:38Z |
| publishDate | 2019-08-01 |
| publisher | Department of Chemistry, Universitas Gadjah Mada |
| record_format | Article |
| series | Indonesian Journal of Chemistry |
| spelling | doaj.art-49ae70dc05a94430a6e69095701b7d0e2022-12-21T22:58:05ZengDepartment of Chemistry, Universitas Gadjah MadaIndonesian Journal of Chemistry1411-94202460-15782019-08-0119490090610.22146/ijc.3751323342Angiotensin Converting Enzyme (ACE) Inhibitory Activity of Peptide Fraction of Germinated Pigeon Pea (<i>Cajanus cajan</i> (L.) Millsp.)Ketut Ratnayani0I Ketut Suter1Nyoman Semadi Antara2I Nengah Kencana Putra3Department of Chemistry, Faculty of Natural Sciences, Udayana University, Denpasar 80361, IndonesiaDepartment of Food Science and Technology, Faculty of Agricultural Technology, Udayana University, Denpasar 80361, IndonesiaDepartment of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University, Denpasar 80361, IndonesiaDepartment of Food Science and Technology, Faculty of Agricultural Technology, Udayana University, Denpasar 80361, IndonesiaDuring the germination process, seeds can release various types of peptides due to the degradation of storage proteins. Some of these peptides can have biological activity (bioactive peptides). The objective of this study was to determine the ACE inhibitory activity of germinated pigeon pea peptide extract at various germination times and to carry out the fractionation to the extract to get the most active peptide fraction. The results showed that the highest activity of peptide extract was found on the 4th-day germination of pigeon pea with an IC50 value of 63.46 μg/mL. The peptide extract was further fractionated by centrifugal ultrafiltration method and it was found that the peptide fraction < 3 kDa had the highest ACE inhibitory activity with an IC50 value of 57.79 μg/mL. The result of identification with the LCMS method to the fraction was able to detect 4 types of the peptide with a molecular weight of 230.304, 294.303, 441.436, and 570.591 Da. These results suggested that the peptide fraction of germinated pigeon pea has the potency as an ACE inhibitory nutraceutical.https://jurnal.ugm.ac.id/ijc/article/view/37513pigeon peapeptide fractionACE inhibitorygermination |
| spellingShingle | Ketut Ratnayani I Ketut Suter Nyoman Semadi Antara I Nengah Kencana Putra Angiotensin Converting Enzyme (ACE) Inhibitory Activity of Peptide Fraction of Germinated Pigeon Pea (<i>Cajanus cajan</i> (L.) Millsp.) Indonesian Journal of Chemistry pigeon pea peptide fraction ACE inhibitory germination |
| title | Angiotensin Converting Enzyme (ACE) Inhibitory Activity of Peptide Fraction of Germinated Pigeon Pea (<i>Cajanus cajan</i> (L.) Millsp.) |
| title_full | Angiotensin Converting Enzyme (ACE) Inhibitory Activity of Peptide Fraction of Germinated Pigeon Pea (<i>Cajanus cajan</i> (L.) Millsp.) |
| title_fullStr | Angiotensin Converting Enzyme (ACE) Inhibitory Activity of Peptide Fraction of Germinated Pigeon Pea (<i>Cajanus cajan</i> (L.) Millsp.) |
| title_full_unstemmed | Angiotensin Converting Enzyme (ACE) Inhibitory Activity of Peptide Fraction of Germinated Pigeon Pea (<i>Cajanus cajan</i> (L.) Millsp.) |
| title_short | Angiotensin Converting Enzyme (ACE) Inhibitory Activity of Peptide Fraction of Germinated Pigeon Pea (<i>Cajanus cajan</i> (L.) Millsp.) |
| title_sort | angiotensin converting enzyme ace inhibitory activity of peptide fraction of germinated pigeon pea i cajanus cajan i l millsp |
| topic | pigeon pea peptide fraction ACE inhibitory germination |
| url | https://jurnal.ugm.ac.id/ijc/article/view/37513 |
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