Relationships between Gut Microbiota, Metabolome, Body Weight, and Glucose Homeostasis of Obese Dogs Fed with Diets Differing in Prebiotic and Protein Content
Obesity is a major issue in pets and nutritional strategies need to be developed, like promoting greater protein and fiber intake. This study aimed to evaluate the effects of dietary protein levels and prebiotic supplementation on the glucose metabolism and relationships between the gut, microbiota,...
| Autori principali: | , , , , , , |
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| Natura: | Articolo |
| Lingua: | English |
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MDPI AG
2020-04-01
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| Serie: | Microorganisms |
| Soggetti: | |
| Accesso online: | https://www.mdpi.com/2076-2607/8/4/513 |
| _version_ | 1827719334686883840 |
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| author | Emmanuelle Apper Lisa Privet Bernard Taminiau Cindy Le Bourgot Ljubica Svilar Jean-Charles Martin Marianne Diez |
| author_facet | Emmanuelle Apper Lisa Privet Bernard Taminiau Cindy Le Bourgot Ljubica Svilar Jean-Charles Martin Marianne Diez |
| author_sort | Emmanuelle Apper |
| collection | DOAJ |
| description | Obesity is a major issue in pets and nutritional strategies need to be developed, like promoting greater protein and fiber intake. This study aimed to evaluate the effects of dietary protein levels and prebiotic supplementation on the glucose metabolism and relationships between the gut, microbiota, metabolome, and phenotype of obese dogs. Six obese Beagle dogs received a diet containing 25.6% or 36.9% crude protein, with or without 1% short-chain fructo-oligosaccharide (scFOS) or oligofructose (OF), in a Latin-square study design. Fecal and blood samples were collected for metabolite analysis, untargeted metabolomics, and 16S rRNA amplicon sequencing. A multi-block analysis was performed to build a correlation network to identify relationships between fecal microbiota, metabolome, and phenotypic variables. Diets did not affect energy homeostasis, but scFOS supplementation modulated fecal microbiota composition and induced significant changes of the fecal metabolome. Bile acids and several amino acids were related to glucose homeostasis while specific bacteria gathered in metavariables had a high number of links with phenotypic and metabolomic parameters. It also suggested that fecal aminoadipate and hippurate act as potential markers of glucose homeostasis. This preliminary study provides new insights into the relationships between the gut microbiota, the metabolome, and several phenotypic markers involved in obesity and associated metabolic dysfunctions. |
| first_indexed | 2024-03-10T20:41:50Z |
| format | Article |
| id | doaj.art-49aefa0db246481b93c42d1d3c5a0be8 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T20:41:50Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-49aefa0db246481b93c42d1d3c5a0be82023-11-19T20:38:11ZengMDPI AGMicroorganisms2076-26072020-04-018451310.3390/microorganisms8040513Relationships between Gut Microbiota, Metabolome, Body Weight, and Glucose Homeostasis of Obese Dogs Fed with Diets Differing in Prebiotic and Protein ContentEmmanuelle Apper0Lisa Privet1Bernard Taminiau2Cindy Le Bourgot3Ljubica Svilar4Jean-Charles Martin5Marianne Diez6Tereos, Research and Innovation, 77230 Moussy-le-Vieux, FranceMS Nutrition, C2VN, INRA, INSERM, Aix-Marseille University, 13385 Marseille, FranceFarah Centre, Department of Food Sciences, University of Liege, 4000 Liège, BelgiumTereos, Research and Innovation, 77230 Moussy-le-Vieux, FranceCRIBIOM, C2VN, INRA, INSERM, Aix-Marseille University, 13385 Marseille, FranceBioMeT, C2VN, INRA, INSERM, Aix-Marseille University, 13385 Marseille, FranceNutrition Unit, Department of Animal Production, Faculty of Veterinary Medicine, University of Liege, 4000 Liège, BelgiumObesity is a major issue in pets and nutritional strategies need to be developed, like promoting greater protein and fiber intake. This study aimed to evaluate the effects of dietary protein levels and prebiotic supplementation on the glucose metabolism and relationships between the gut, microbiota, metabolome, and phenotype of obese dogs. Six obese Beagle dogs received a diet containing 25.6% or 36.9% crude protein, with or without 1% short-chain fructo-oligosaccharide (scFOS) or oligofructose (OF), in a Latin-square study design. Fecal and blood samples were collected for metabolite analysis, untargeted metabolomics, and 16S rRNA amplicon sequencing. A multi-block analysis was performed to build a correlation network to identify relationships between fecal microbiota, metabolome, and phenotypic variables. Diets did not affect energy homeostasis, but scFOS supplementation modulated fecal microbiota composition and induced significant changes of the fecal metabolome. Bile acids and several amino acids were related to glucose homeostasis while specific bacteria gathered in metavariables had a high number of links with phenotypic and metabolomic parameters. It also suggested that fecal aminoadipate and hippurate act as potential markers of glucose homeostasis. This preliminary study provides new insights into the relationships between the gut microbiota, the metabolome, and several phenotypic markers involved in obesity and associated metabolic dysfunctions.https://www.mdpi.com/2076-2607/8/4/513prebioticobesityenergy homeostasismicrobiotametabolomebile acids |
| spellingShingle | Emmanuelle Apper Lisa Privet Bernard Taminiau Cindy Le Bourgot Ljubica Svilar Jean-Charles Martin Marianne Diez Relationships between Gut Microbiota, Metabolome, Body Weight, and Glucose Homeostasis of Obese Dogs Fed with Diets Differing in Prebiotic and Protein Content Microorganisms prebiotic obesity energy homeostasis microbiota metabolome bile acids |
| title | Relationships between Gut Microbiota, Metabolome, Body Weight, and Glucose Homeostasis of Obese Dogs Fed with Diets Differing in Prebiotic and Protein Content |
| title_full | Relationships between Gut Microbiota, Metabolome, Body Weight, and Glucose Homeostasis of Obese Dogs Fed with Diets Differing in Prebiotic and Protein Content |
| title_fullStr | Relationships between Gut Microbiota, Metabolome, Body Weight, and Glucose Homeostasis of Obese Dogs Fed with Diets Differing in Prebiotic and Protein Content |
| title_full_unstemmed | Relationships between Gut Microbiota, Metabolome, Body Weight, and Glucose Homeostasis of Obese Dogs Fed with Diets Differing in Prebiotic and Protein Content |
| title_short | Relationships between Gut Microbiota, Metabolome, Body Weight, and Glucose Homeostasis of Obese Dogs Fed with Diets Differing in Prebiotic and Protein Content |
| title_sort | relationships between gut microbiota metabolome body weight and glucose homeostasis of obese dogs fed with diets differing in prebiotic and protein content |
| topic | prebiotic obesity energy homeostasis microbiota metabolome bile acids |
| url | https://www.mdpi.com/2076-2607/8/4/513 |
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