Gold island‐enhanced multiplex quantum dots fluorescent system for biomedical analysis of circulating tumor nucleic acids
Abstract Circulating tumor nucleic acids (CTNAs) have been employed as the potential markers for tumor diagnosis and management, which are highly correlated with the tumorigenesis, progression, and metastasis processes. Therefore, it is important to develop a highly‐sensitive and reliable method for...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2022-12-01
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| Series: | Nano Select |
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| Online Access: | https://doi.org/10.1002/nano.202200172 |
| _version_ | 1811205346644983808 |
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| author | Jingyan Lin Yunxia Wu Jingru Wang Ronghua Yang Wenjie Wu Bin Li Xiaodong Chen Wei Xue Yuhui Liao |
| author_facet | Jingyan Lin Yunxia Wu Jingru Wang Ronghua Yang Wenjie Wu Bin Li Xiaodong Chen Wei Xue Yuhui Liao |
| author_sort | Jingyan Lin |
| collection | DOAJ |
| description | Abstract Circulating tumor nucleic acids (CTNAs) have been employed as the potential markers for tumor diagnosis and management, which are highly correlated with the tumorigenesis, progression, and metastasis processes. Therefore, it is important to develop a highly‐sensitive and reliable method for the detection of CTNAs, especially the multiplex point mutation detection of blood‐derived CTNAs. Herein, a versatile gold island‐enhanced multiplex quantum dots (GIEM‐QDs) fluorescent platform was constructed for the highly‐sensitive detection of CTNAs in serum. The GIEM fluorescent strategy was designed as the highly‐efficient signal giving‐out mode which could amplify the fluorescence intensity of QDs, thus realizing a homogeneous platform for the enrichment, multiplex detection, and point mutations monitoring of CTNAs with the principle of base‐stacking. A high sensitivity of 10 amol and desirable specificity was achieved, and the performance index for analysis of clinical tumor patients’ samples indicated that the GIEM‐QDs fluorescent strategy could realize multiplex point mutation detection of CTNAs in complex blood samples. Hence, this platform could achieve a high detection rate in clinical samples that suitably met the clinical requirements for multiplex detection and point mutations monitoring of CTNAs, and thus has the potential to serve as the tumor liquid biopsy strategy based on CTNAs. |
| first_indexed | 2024-04-12T03:29:26Z |
| format | Article |
| id | doaj.art-49b0a41c721642a4951bac34dec0cdb9 |
| institution | Directory Open Access Journal |
| issn | 2688-4011 |
| language | English |
| last_indexed | 2024-04-12T03:29:26Z |
| publishDate | 2022-12-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Nano Select |
| spelling | doaj.art-49b0a41c721642a4951bac34dec0cdb92022-12-22T03:49:35ZengWiley-VCHNano Select2688-40112022-12-013121588159710.1002/nano.202200172Gold island‐enhanced multiplex quantum dots fluorescent system for biomedical analysis of circulating tumor nucleic acidsJingyan Lin0Yunxia Wu1Jingru Wang2Ronghua Yang3Wenjie Wu4Bin Li5Xiaodong Chen6Wei Xue7Yuhui Liao8Department of Thoracic Surgery Shenzhen Third People's Hospital Shenzhen ChinaKey Laboratory of Biomaterials of Guangdong Higher Education Institutes Guangdong Provincial Engineering and Technological Research Center for Drug Carrier Development Department of Biomedical Engineering Jinan University Guangzhou ChinaDepartment of Burn Surgery The First People's Hospital of Foshan Foshan ChinaDepartment of Burn and Plastic Surgery Guangzhou First People's Hospital, South China University of Technology Guangzhou ChinaMolecular Diagnosis and Treatment Center for Infectious Diseases Dermatology Hospital of Southern Medical University Guangzhou ChinaDepartment of Burn Surgery The First People's Hospital of Foshan Foshan ChinaDepartment of Burn Surgery The First People's Hospital of Foshan Foshan ChinaKey Laboratory of Biomaterials of Guangdong Higher Education Institutes Guangdong Provincial Engineering and Technological Research Center for Drug Carrier Development Department of Biomedical Engineering Jinan University Guangzhou ChinaMolecular Diagnosis and Treatment Center for Infectious Diseases Dermatology Hospital of Southern Medical University Guangzhou ChinaAbstract Circulating tumor nucleic acids (CTNAs) have been employed as the potential markers for tumor diagnosis and management, which are highly correlated with the tumorigenesis, progression, and metastasis processes. Therefore, it is important to develop a highly‐sensitive and reliable method for the detection of CTNAs, especially the multiplex point mutation detection of blood‐derived CTNAs. Herein, a versatile gold island‐enhanced multiplex quantum dots (GIEM‐QDs) fluorescent platform was constructed for the highly‐sensitive detection of CTNAs in serum. The GIEM fluorescent strategy was designed as the highly‐efficient signal giving‐out mode which could amplify the fluorescence intensity of QDs, thus realizing a homogeneous platform for the enrichment, multiplex detection, and point mutations monitoring of CTNAs with the principle of base‐stacking. A high sensitivity of 10 amol and desirable specificity was achieved, and the performance index for analysis of clinical tumor patients’ samples indicated that the GIEM‐QDs fluorescent strategy could realize multiplex point mutation detection of CTNAs in complex blood samples. Hence, this platform could achieve a high detection rate in clinical samples that suitably met the clinical requirements for multiplex detection and point mutations monitoring of CTNAs, and thus has the potential to serve as the tumor liquid biopsy strategy based on CTNAs.https://doi.org/10.1002/nano.202200172circulating tumor nucleic acidsgold islandmultiplex detectionquantum dots |
| spellingShingle | Jingyan Lin Yunxia Wu Jingru Wang Ronghua Yang Wenjie Wu Bin Li Xiaodong Chen Wei Xue Yuhui Liao Gold island‐enhanced multiplex quantum dots fluorescent system for biomedical analysis of circulating tumor nucleic acids Nano Select circulating tumor nucleic acids gold island multiplex detection quantum dots |
| title | Gold island‐enhanced multiplex quantum dots fluorescent system for biomedical analysis of circulating tumor nucleic acids |
| title_full | Gold island‐enhanced multiplex quantum dots fluorescent system for biomedical analysis of circulating tumor nucleic acids |
| title_fullStr | Gold island‐enhanced multiplex quantum dots fluorescent system for biomedical analysis of circulating tumor nucleic acids |
| title_full_unstemmed | Gold island‐enhanced multiplex quantum dots fluorescent system for biomedical analysis of circulating tumor nucleic acids |
| title_short | Gold island‐enhanced multiplex quantum dots fluorescent system for biomedical analysis of circulating tumor nucleic acids |
| title_sort | gold island enhanced multiplex quantum dots fluorescent system for biomedical analysis of circulating tumor nucleic acids |
| topic | circulating tumor nucleic acids gold island multiplex detection quantum dots |
| url | https://doi.org/10.1002/nano.202200172 |
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