Electrochemical Biosensing of L-DOPA Using Tyrosinase Immobilized on Carboxymethyl Starch-<i>Graft</i>-Polyaniline@MWCNTs Nanocomposite
The electrochemical behavior of the immobilized tyrosinase (Tyrase) on a modified glassy carbon electrode with carboxymethyl starch-<i>graft</i>-polyaniline/multi-walled carbon nanotubes nanocomposite (CMS-<i>g</i>-PANI@MWCNTs) was investigated. The molecular properties of CM...
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MDPI AG
2023-05-01
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author | Fahimeh Mollamohammadi Hassan Faridnouri Ehsan Nazarzadeh Zare |
author_facet | Fahimeh Mollamohammadi Hassan Faridnouri Ehsan Nazarzadeh Zare |
author_sort | Fahimeh Mollamohammadi |
collection | DOAJ |
description | The electrochemical behavior of the immobilized tyrosinase (Tyrase) on a modified glassy carbon electrode with carboxymethyl starch-<i>graft</i>-polyaniline/multi-walled carbon nanotubes nanocomposite (CMS-<i>g</i>-PANI@MWCNTs) was investigated. The molecular properties of CMS-<i>g</i>-PANI@MWCNTs nanocomposite and its morphological characterization were examined by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and field emission scanning electron microscopy (FESEM). A simple drop-casting method was employed to immobilize Tyrase on the CMS-<i>g</i>-PANI@MWCNTs nanocomposite. In the cyclic voltammogram (CV), a pair of redox peaks were observed at the potentials of +0.25 to −0.1 V and E°’ was equal to 0.1 V and the apparent rate constant of electron transfer (K<sub>s</sub>) was calculated at 0.4 s<sup>−1</sup>. Using differential pulse voltammetry (DPV), the sensitivity and selectivity of the biosensor were investigated. The biosensor exhibits linearity towards catechol and L-dopa in the concentration range of 5–100 and 10–300 μM with a sensitivity of 2.4 and 1.11 μA μΜ<sup>−1</sup> cm<sup>−2</sup> and limit of detection (LOD) 25 and 30 μM, respectively. The Michaelis-Menten constant (K<sub>m</sub>) was calculated at 42 μΜ for catechol and 86 μΜ for L-dopa. After 28 working days, the biosensor provided good repeatability and selectivity, and maintained 67% of its stability. The existence of -COO<sup>−</sup> and -OH groups in carboxymethyl starch, -NH<sub>2</sub> groups in polyaniline, and high surface-to-volume ratio and electrical conductivity of multi-walled carbon nanotubes in the CMS-<i>g</i>-PANI@MWCNTs nanocomposite cause good Tyrase immobilization on the surface of the electrode. |
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language | English |
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spelling | doaj.art-49b166682a274a2f878ebdce3c22f7392023-11-18T00:41:28ZengMDPI AGBiosensors2079-63742023-05-0113556210.3390/bios13050562Electrochemical Biosensing of L-DOPA Using Tyrosinase Immobilized on Carboxymethyl Starch-<i>Graft</i>-Polyaniline@MWCNTs NanocompositeFahimeh Mollamohammadi0Hassan Faridnouri1Ehsan Nazarzadeh Zare2School of Biology, Damghan University, Damghan 36716-45667, IranSchool of Biology, Damghan University, Damghan 36716-45667, IranSchool of Chemistry, Damghan University, Damghan 36716-45667, IranThe electrochemical behavior of the immobilized tyrosinase (Tyrase) on a modified glassy carbon electrode with carboxymethyl starch-<i>graft</i>-polyaniline/multi-walled carbon nanotubes nanocomposite (CMS-<i>g</i>-PANI@MWCNTs) was investigated. The molecular properties of CMS-<i>g</i>-PANI@MWCNTs nanocomposite and its morphological characterization were examined by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and field emission scanning electron microscopy (FESEM). A simple drop-casting method was employed to immobilize Tyrase on the CMS-<i>g</i>-PANI@MWCNTs nanocomposite. In the cyclic voltammogram (CV), a pair of redox peaks were observed at the potentials of +0.25 to −0.1 V and E°’ was equal to 0.1 V and the apparent rate constant of electron transfer (K<sub>s</sub>) was calculated at 0.4 s<sup>−1</sup>. Using differential pulse voltammetry (DPV), the sensitivity and selectivity of the biosensor were investigated. The biosensor exhibits linearity towards catechol and L-dopa in the concentration range of 5–100 and 10–300 μM with a sensitivity of 2.4 and 1.11 μA μΜ<sup>−1</sup> cm<sup>−2</sup> and limit of detection (LOD) 25 and 30 μM, respectively. The Michaelis-Menten constant (K<sub>m</sub>) was calculated at 42 μΜ for catechol and 86 μΜ for L-dopa. After 28 working days, the biosensor provided good repeatability and selectivity, and maintained 67% of its stability. The existence of -COO<sup>−</sup> and -OH groups in carboxymethyl starch, -NH<sub>2</sub> groups in polyaniline, and high surface-to-volume ratio and electrical conductivity of multi-walled carbon nanotubes in the CMS-<i>g</i>-PANI@MWCNTs nanocomposite cause good Tyrase immobilization on the surface of the electrode.https://www.mdpi.com/2079-6374/13/5/562carboxymethyl starchpolyanilineMWCNTstyrosinaseelectrical nanocomposite |
spellingShingle | Fahimeh Mollamohammadi Hassan Faridnouri Ehsan Nazarzadeh Zare Electrochemical Biosensing of L-DOPA Using Tyrosinase Immobilized on Carboxymethyl Starch-<i>Graft</i>-Polyaniline@MWCNTs Nanocomposite Biosensors carboxymethyl starch polyaniline MWCNTs tyrosinase electrical nanocomposite |
title | Electrochemical Biosensing of L-DOPA Using Tyrosinase Immobilized on Carboxymethyl Starch-<i>Graft</i>-Polyaniline@MWCNTs Nanocomposite |
title_full | Electrochemical Biosensing of L-DOPA Using Tyrosinase Immobilized on Carboxymethyl Starch-<i>Graft</i>-Polyaniline@MWCNTs Nanocomposite |
title_fullStr | Electrochemical Biosensing of L-DOPA Using Tyrosinase Immobilized on Carboxymethyl Starch-<i>Graft</i>-Polyaniline@MWCNTs Nanocomposite |
title_full_unstemmed | Electrochemical Biosensing of L-DOPA Using Tyrosinase Immobilized on Carboxymethyl Starch-<i>Graft</i>-Polyaniline@MWCNTs Nanocomposite |
title_short | Electrochemical Biosensing of L-DOPA Using Tyrosinase Immobilized on Carboxymethyl Starch-<i>Graft</i>-Polyaniline@MWCNTs Nanocomposite |
title_sort | electrochemical biosensing of l dopa using tyrosinase immobilized on carboxymethyl starch i graft i polyaniline mwcnts nanocomposite |
topic | carboxymethyl starch polyaniline MWCNTs tyrosinase electrical nanocomposite |
url | https://www.mdpi.com/2079-6374/13/5/562 |
work_keys_str_mv | AT fahimehmollamohammadi electrochemicalbiosensingofldopausingtyrosinaseimmobilizedoncarboxymethylstarchigraftipolyanilinemwcntsnanocomposite AT hassanfaridnouri electrochemicalbiosensingofldopausingtyrosinaseimmobilizedoncarboxymethylstarchigraftipolyanilinemwcntsnanocomposite AT ehsannazarzadehzare electrochemicalbiosensingofldopausingtyrosinaseimmobilizedoncarboxymethylstarchigraftipolyanilinemwcntsnanocomposite |