Antigenicity and Immunogenicity Analysis of the <em>E. coli</em> Expressed FMDV Structural Proteins; VP1, VP0, VP3 of the South African Territories Type 2 Virus
An alternative vaccine design approach and diagnostic kits are highly required against the anticipated pandemicity caused by the South African Territories type 2 (SAT2) Foot and Mouth Disease Virus (FMDV). However, the distinct antigenicity and immunogenicity of VP1, VP0, and VP3 of FMDV serotype SA...
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2021-05-01
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| Online Access: | https://www.mdpi.com/1999-4915/13/6/1005 |
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| author | Guoxiu Li Ashenafi Kiros Wubshet Yaozhong Ding Qian Li Junfei Dai Yang Wang Qian Hou Jiao Chen Bing Ma Anna Szczotka-Bochniarz Susan Szathmary Yongguang Zhang Jie Zhang |
| author_facet | Guoxiu Li Ashenafi Kiros Wubshet Yaozhong Ding Qian Li Junfei Dai Yang Wang Qian Hou Jiao Chen Bing Ma Anna Szczotka-Bochniarz Susan Szathmary Yongguang Zhang Jie Zhang |
| author_sort | Guoxiu Li |
| collection | DOAJ |
| description | An alternative vaccine design approach and diagnostic kits are highly required against the anticipated pandemicity caused by the South African Territories type 2 (SAT2) Foot and Mouth Disease Virus (FMDV). However, the distinct antigenicity and immunogenicity of VP1, VP0, and VP3 of FMDV serotype SAT2 are poorly understood. Similarly, the particular roles of the three structural proteins in novel vaccine design and development remain unexplained. We therefore constructed VP1, VP0, and VP3 encoding gene (SAT2:JX014256 strain) separately fused with <i>His-SUMO</i> (histidine-small ubiquitin-related modifier) inserted into pET-32a cassette to express the three recombinant proteins and separately evaluated their antigenicity and immunogenicity in mice. The fusion protein was successfully expressed and purified by the Ni-NTA resin chromatography. The level of serum antibody, spleen lymphocyte proliferation, and cytokines against the three distinct recombinant proteins were analyzed. Results showed that the anti-FMDV humoral response was triggered by these proteins, and the fusion proteins did enhance the splenocyte immune response in the separately immunized mice. We observed low variations among the three fusion proteins in terms of the antibody and cytokine production in mice. Hence, in this study, results demonstrated that the structural proteins of SAT2 FMDV could be used for the development of immunodiagnostic kits and subunit vaccine designs. |
| first_indexed | 2024-03-10T10:58:26Z |
| format | Article |
| id | doaj.art-49b46cffa8d94a5a84dc2a9ab43c5862 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T10:58:26Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-49b46cffa8d94a5a84dc2a9ab43c58622023-11-21T21:40:39ZengMDPI AGViruses1999-49152021-05-01136100510.3390/v13061005Antigenicity and Immunogenicity Analysis of the <em>E. coli</em> Expressed FMDV Structural Proteins; VP1, VP0, VP3 of the South African Territories Type 2 VirusGuoxiu Li0Ashenafi Kiros Wubshet1Yaozhong Ding2Qian Li3Junfei Dai4Yang Wang5Qian Hou6Jiao Chen7Bing Ma8Anna Szczotka-Bochniarz9Susan Szathmary10Yongguang Zhang11Jie Zhang12State Key Laboratory of Veterinary Etiological Biology, National/OIE Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, ChinaState Key Laboratory of Veterinary Etiological Biology, National/OIE Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, ChinaState Key Laboratory of Veterinary Etiological Biology, National/OIE Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, ChinaState Key Laboratory of Veterinary Etiological Biology, National/OIE Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, ChinaState Key Laboratory of Veterinary Etiological Biology, National/OIE Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, ChinaState Key Laboratory of Veterinary Etiological Biology, National/OIE Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, ChinaState Key Laboratory of Veterinary Etiological Biology, National/OIE Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, ChinaState Key Laboratory of Veterinary Etiological Biology, National/OIE Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, ChinaState Key Laboratory of Veterinary Etiological Biology, National/OIE Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, ChinaDepartment of Swine Diseases, National Veterinary Research Institute, 57 Partyzantow, 24-100 Puławy, PolandRT-Europe Research Center, H-9200 Mosonmagyarovar, HungaryState Key Laboratory of Veterinary Etiological Biology, National/OIE Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, ChinaState Key Laboratory of Veterinary Etiological Biology, National/OIE Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, ChinaAn alternative vaccine design approach and diagnostic kits are highly required against the anticipated pandemicity caused by the South African Territories type 2 (SAT2) Foot and Mouth Disease Virus (FMDV). However, the distinct antigenicity and immunogenicity of VP1, VP0, and VP3 of FMDV serotype SAT2 are poorly understood. Similarly, the particular roles of the three structural proteins in novel vaccine design and development remain unexplained. We therefore constructed VP1, VP0, and VP3 encoding gene (SAT2:JX014256 strain) separately fused with <i>His-SUMO</i> (histidine-small ubiquitin-related modifier) inserted into pET-32a cassette to express the three recombinant proteins and separately evaluated their antigenicity and immunogenicity in mice. The fusion protein was successfully expressed and purified by the Ni-NTA resin chromatography. The level of serum antibody, spleen lymphocyte proliferation, and cytokines against the three distinct recombinant proteins were analyzed. Results showed that the anti-FMDV humoral response was triggered by these proteins, and the fusion proteins did enhance the splenocyte immune response in the separately immunized mice. We observed low variations among the three fusion proteins in terms of the antibody and cytokine production in mice. Hence, in this study, results demonstrated that the structural proteins of SAT2 FMDV could be used for the development of immunodiagnostic kits and subunit vaccine designs.https://www.mdpi.com/1999-4915/13/6/1005FMDVSAT2structural proteinsantigenicityimmunogenicityvaccine |
| spellingShingle | Guoxiu Li Ashenafi Kiros Wubshet Yaozhong Ding Qian Li Junfei Dai Yang Wang Qian Hou Jiao Chen Bing Ma Anna Szczotka-Bochniarz Susan Szathmary Yongguang Zhang Jie Zhang Antigenicity and Immunogenicity Analysis of the <em>E. coli</em> Expressed FMDV Structural Proteins; VP1, VP0, VP3 of the South African Territories Type 2 Virus Viruses FMDV SAT2 structural proteins antigenicity immunogenicity vaccine |
| title | Antigenicity and Immunogenicity Analysis of the <em>E. coli</em> Expressed FMDV Structural Proteins; VP1, VP0, VP3 of the South African Territories Type 2 Virus |
| title_full | Antigenicity and Immunogenicity Analysis of the <em>E. coli</em> Expressed FMDV Structural Proteins; VP1, VP0, VP3 of the South African Territories Type 2 Virus |
| title_fullStr | Antigenicity and Immunogenicity Analysis of the <em>E. coli</em> Expressed FMDV Structural Proteins; VP1, VP0, VP3 of the South African Territories Type 2 Virus |
| title_full_unstemmed | Antigenicity and Immunogenicity Analysis of the <em>E. coli</em> Expressed FMDV Structural Proteins; VP1, VP0, VP3 of the South African Territories Type 2 Virus |
| title_short | Antigenicity and Immunogenicity Analysis of the <em>E. coli</em> Expressed FMDV Structural Proteins; VP1, VP0, VP3 of the South African Territories Type 2 Virus |
| title_sort | antigenicity and immunogenicity analysis of the em e coli em expressed fmdv structural proteins vp1 vp0 vp3 of the south african territories type 2 virus |
| topic | FMDV SAT2 structural proteins antigenicity immunogenicity vaccine |
| url | https://www.mdpi.com/1999-4915/13/6/1005 |
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