Effects of overexpression of IL-10, IL-12, TGF-β and IL-4 on allergen induced change in bronchial responsiveness
<p>Abstract</p> <p>Background</p> <p>An increasing prevalence of allergic diseases, such as atopic dermatitis, allergic rhinitis and bronchial asthma, has been noted worldwide. Allergic asthma strongly correlates with airway inflammation caused by the unregulated produc...
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| Format: | Article |
| Language: | English |
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BMC
2006-05-01
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| Series: | Respiratory Research |
| Online Access: | http://respiratory-research.com/content/7/1/72 |
| _version_ | 1818067818062544896 |
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| author | Lee Yueh-Lun Ye Yi-Ling Fu Chi-Ling Chiang Bor-Luen |
| author_facet | Lee Yueh-Lun Ye Yi-Ling Fu Chi-Ling Chiang Bor-Luen |
| author_sort | Lee Yueh-Lun |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>An increasing prevalence of allergic diseases, such as atopic dermatitis, allergic rhinitis and bronchial asthma, has been noted worldwide. Allergic asthma strongly correlates with airway inflammation caused by the unregulated production of cytokines secreted by allergen-specific type-2 T helper (Th2) cells. This study aims to explore the therapeutic effect of the airway gene transfer of IL-12, IL-10 and TGF-β on airway inflammation in a mouse model of allergic asthma.</p> <p>Methods</p> <p>BALB/c mice were sensitized to ovalbumin (OVA) by intraperitoneal injections with OVA and challenged by nebulized OVA. Different cytokine gene plasmids or non-coding vector plasmids were instilled daily into the trachea up to one day before the inhalatory OVA challenge phase.</p> <p>Results</p> <p>Intratracheal administration of IL-10, IL-12 or TGF-β can efficiently inhibit antigen-induced airway hyper-responsiveness and is able to largely significantly lower the number of eosinophils and neutrophils in bronchoalveolar lavage fluid of ovalbumin (OVA) sensitized and challenged mice during the effector phase. Furthermore, the effect of IL-10 plasmids is more remarkable than any other cytokine gene plasmid. On the other hand, local administration of IL-4 gene plasmids before antigen challenge can induce severe airway hyper-responsiveness (AHR) and airway eosinophilia.</p> <p>Conclusion</p> <p>Our data demonstrated that anti- inflammatory cytokines, particularly IL-10, have the therapeutic potential for the alleviation of airway inflammation in murine model of asthma.</p> |
| first_indexed | 2024-12-10T15:29:43Z |
| format | Article |
| id | doaj.art-49b52431c43345a1bc1a24aae163000e |
| institution | Directory Open Access Journal |
| issn | 1465-9921 |
| language | English |
| last_indexed | 2024-12-10T15:29:43Z |
| publishDate | 2006-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Respiratory Research |
| spelling | doaj.art-49b52431c43345a1bc1a24aae163000e2022-12-22T01:43:26ZengBMCRespiratory Research1465-99212006-05-01717210.1186/1465-9921-7-72Effects of overexpression of IL-10, IL-12, TGF-β and IL-4 on allergen induced change in bronchial responsivenessLee Yueh-LunYe Yi-LingFu Chi-LingChiang Bor-Luen<p>Abstract</p> <p>Background</p> <p>An increasing prevalence of allergic diseases, such as atopic dermatitis, allergic rhinitis and bronchial asthma, has been noted worldwide. Allergic asthma strongly correlates with airway inflammation caused by the unregulated production of cytokines secreted by allergen-specific type-2 T helper (Th2) cells. This study aims to explore the therapeutic effect of the airway gene transfer of IL-12, IL-10 and TGF-β on airway inflammation in a mouse model of allergic asthma.</p> <p>Methods</p> <p>BALB/c mice were sensitized to ovalbumin (OVA) by intraperitoneal injections with OVA and challenged by nebulized OVA. Different cytokine gene plasmids or non-coding vector plasmids were instilled daily into the trachea up to one day before the inhalatory OVA challenge phase.</p> <p>Results</p> <p>Intratracheal administration of IL-10, IL-12 or TGF-β can efficiently inhibit antigen-induced airway hyper-responsiveness and is able to largely significantly lower the number of eosinophils and neutrophils in bronchoalveolar lavage fluid of ovalbumin (OVA) sensitized and challenged mice during the effector phase. Furthermore, the effect of IL-10 plasmids is more remarkable than any other cytokine gene plasmid. On the other hand, local administration of IL-4 gene plasmids before antigen challenge can induce severe airway hyper-responsiveness (AHR) and airway eosinophilia.</p> <p>Conclusion</p> <p>Our data demonstrated that anti- inflammatory cytokines, particularly IL-10, have the therapeutic potential for the alleviation of airway inflammation in murine model of asthma.</p>http://respiratory-research.com/content/7/1/72 |
| spellingShingle | Lee Yueh-Lun Ye Yi-Ling Fu Chi-Ling Chiang Bor-Luen Effects of overexpression of IL-10, IL-12, TGF-β and IL-4 on allergen induced change in bronchial responsiveness Respiratory Research |
| title | Effects of overexpression of IL-10, IL-12, TGF-β and IL-4 on allergen induced change in bronchial responsiveness |
| title_full | Effects of overexpression of IL-10, IL-12, TGF-β and IL-4 on allergen induced change in bronchial responsiveness |
| title_fullStr | Effects of overexpression of IL-10, IL-12, TGF-β and IL-4 on allergen induced change in bronchial responsiveness |
| title_full_unstemmed | Effects of overexpression of IL-10, IL-12, TGF-β and IL-4 on allergen induced change in bronchial responsiveness |
| title_short | Effects of overexpression of IL-10, IL-12, TGF-β and IL-4 on allergen induced change in bronchial responsiveness |
| title_sort | effects of overexpression of il 10 il 12 tgf β and il 4 on allergen induced change in bronchial responsiveness |
| url | http://respiratory-research.com/content/7/1/72 |
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