Activation of the VQ Motif-Containing Protein Gene <i>VQ28</i> Compromised Nonhost Resistance of <i>Arabidopsis thaliana</i> to <i>Phytophthora</i> Pathogens
Nonhost resistance refers to resistance of a plant species to all genetic variants of a non-adapted pathogen. Such resistance has the potential to become broad-spectrum and durable crop disease resistance. We previously employed <i>Arabidopsis thaliana</i> and a forward genetics approach...
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MDPI AG
2022-03-01
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| Series: | Plants |
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| author | Xingjie Lan Xiaoxia Wang Quandan Tao Haotian Zhang Jinyang Li Yuling Meng Weixing Shan |
| author_facet | Xingjie Lan Xiaoxia Wang Quandan Tao Haotian Zhang Jinyang Li Yuling Meng Weixing Shan |
| author_sort | Xingjie Lan |
| collection | DOAJ |
| description | Nonhost resistance refers to resistance of a plant species to all genetic variants of a non-adapted pathogen. Such resistance has the potential to become broad-spectrum and durable crop disease resistance. We previously employed <i>Arabidopsis thaliana</i> and a forward genetics approach to identify plant mutants susceptible to the nonhost pathogen <i>Phytophthora sojae</i>, which resulted in identification of the T-DNA insertion mutant <i>esp1</i> (<i>enhanced susceptibility to Phytophthora</i>). In this study, we report the identification of VQ motif-containing protein 28 (<i>VQ28</i>), whose expression was highly up-regulated in the mutant <i>esp1</i>. Stable transgenic <i>A. thaliana</i> plants constitutively overexpressing <i>VQ28</i> compromised nonhost resistance (NHR) against <i>P. sojae</i> and <i>P. infestans</i>, and supported increased infection of <i>P. parasitica</i>. Transcriptomic analysis showed that overexpression of <i>VQ28</i> resulted in six differentially expressed genes (DEGs) that are involved in the response to abscisic acid (ABA). High performance liquid chromatography-mass spectrometry (HPLC-MS) detection showed that the contents of endogenous ABA, salicylic acid (SA), and jasmonate (JA) were enriched in <i>VQ28</i> overexpression lines. These findings suggest that overexpression of <i>VQ28</i> may lead to an imbalance in plant hormone homeostasis. Furthermore, transient overexpression of <i>VQ28</i> in <i>Nicotiana benthamiana</i> rendered plants more susceptible to <i>Phytophthora</i> pathogens. Deletion mutant analysis showed that the C-terminus and VQ-motif were essential for plant susceptibility. Taken together, our results suggest that <i>VQ28</i> negatively regulates plant NHR to <i>Phytophthora</i> pathogens. |
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| spelling | doaj.art-49b5c06fe5c24fd1b9bd894f7d1424db2023-11-30T23:49:58ZengMDPI AGPlants2223-77472022-03-0111785810.3390/plants11070858Activation of the VQ Motif-Containing Protein Gene <i>VQ28</i> Compromised Nonhost Resistance of <i>Arabidopsis thaliana</i> to <i>Phytophthora</i> PathogensXingjie Lan0Xiaoxia Wang1Quandan Tao2Haotian Zhang3Jinyang Li4Yuling Meng5Weixing Shan6State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Xianyang 712100, ChinaNonhost resistance refers to resistance of a plant species to all genetic variants of a non-adapted pathogen. Such resistance has the potential to become broad-spectrum and durable crop disease resistance. We previously employed <i>Arabidopsis thaliana</i> and a forward genetics approach to identify plant mutants susceptible to the nonhost pathogen <i>Phytophthora sojae</i>, which resulted in identification of the T-DNA insertion mutant <i>esp1</i> (<i>enhanced susceptibility to Phytophthora</i>). In this study, we report the identification of VQ motif-containing protein 28 (<i>VQ28</i>), whose expression was highly up-regulated in the mutant <i>esp1</i>. Stable transgenic <i>A. thaliana</i> plants constitutively overexpressing <i>VQ28</i> compromised nonhost resistance (NHR) against <i>P. sojae</i> and <i>P. infestans</i>, and supported increased infection of <i>P. parasitica</i>. Transcriptomic analysis showed that overexpression of <i>VQ28</i> resulted in six differentially expressed genes (DEGs) that are involved in the response to abscisic acid (ABA). High performance liquid chromatography-mass spectrometry (HPLC-MS) detection showed that the contents of endogenous ABA, salicylic acid (SA), and jasmonate (JA) were enriched in <i>VQ28</i> overexpression lines. These findings suggest that overexpression of <i>VQ28</i> may lead to an imbalance in plant hormone homeostasis. Furthermore, transient overexpression of <i>VQ28</i> in <i>Nicotiana benthamiana</i> rendered plants more susceptible to <i>Phytophthora</i> pathogens. Deletion mutant analysis showed that the C-terminus and VQ-motif were essential for plant susceptibility. Taken together, our results suggest that <i>VQ28</i> negatively regulates plant NHR to <i>Phytophthora</i> pathogens.https://www.mdpi.com/2223-7747/11/7/858plant immunitynonhost resistancesusceptibility<i>VQ28</i><i>Phytophthora</i> |
| spellingShingle | Xingjie Lan Xiaoxia Wang Quandan Tao Haotian Zhang Jinyang Li Yuling Meng Weixing Shan Activation of the VQ Motif-Containing Protein Gene <i>VQ28</i> Compromised Nonhost Resistance of <i>Arabidopsis thaliana</i> to <i>Phytophthora</i> Pathogens Plants plant immunity nonhost resistance susceptibility <i>VQ28</i> <i>Phytophthora</i> |
| title | Activation of the VQ Motif-Containing Protein Gene <i>VQ28</i> Compromised Nonhost Resistance of <i>Arabidopsis thaliana</i> to <i>Phytophthora</i> Pathogens |
| title_full | Activation of the VQ Motif-Containing Protein Gene <i>VQ28</i> Compromised Nonhost Resistance of <i>Arabidopsis thaliana</i> to <i>Phytophthora</i> Pathogens |
| title_fullStr | Activation of the VQ Motif-Containing Protein Gene <i>VQ28</i> Compromised Nonhost Resistance of <i>Arabidopsis thaliana</i> to <i>Phytophthora</i> Pathogens |
| title_full_unstemmed | Activation of the VQ Motif-Containing Protein Gene <i>VQ28</i> Compromised Nonhost Resistance of <i>Arabidopsis thaliana</i> to <i>Phytophthora</i> Pathogens |
| title_short | Activation of the VQ Motif-Containing Protein Gene <i>VQ28</i> Compromised Nonhost Resistance of <i>Arabidopsis thaliana</i> to <i>Phytophthora</i> Pathogens |
| title_sort | activation of the vq motif containing protein gene i vq28 i compromised nonhost resistance of i arabidopsis thaliana i to i phytophthora i pathogens |
| topic | plant immunity nonhost resistance susceptibility <i>VQ28</i> <i>Phytophthora</i> |
| url | https://www.mdpi.com/2223-7747/11/7/858 |
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