Could Neutrophil CD64 Expression Be Used as a Diagnostic Parameter of Bacteremia in Patients with Febrile Neutropenia?
Objective: The aim of this study is to investigate if neutrophil CD64 expression in febrile neutropenia patients could be used as an early indicator of bacteremia. Materials and Methods: All consecutive patients older than 18 years of age who had developed febrile neutropenia episodes due to hematol...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Galenos Publishing House
2017-05-01
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| Series: | Turkish Journal of Hematology |
| Subjects: | |
| Online Access: | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-82246 |
| _version_ | 1828029392040755200 |
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| author | Nur Efe İris Taner Yıldırmak Habip Gedik Funda Şimşek Demet Aydın Naciye Demirel Osman Yokuş |
| author_facet | Nur Efe İris Taner Yıldırmak Habip Gedik Funda Şimşek Demet Aydın Naciye Demirel Osman Yokuş |
| author_sort | Nur Efe İris |
| collection | DOAJ |
| description | Objective: The aim of this study is to investigate if neutrophil CD64 expression in febrile neutropenia patients could be used as an early indicator of bacteremia. Materials and Methods: All consecutive patients older than 18 years of age who had developed febrile neutropenia episodes due to hematological malignancies were included in the study. Those patients who had significant growth in their blood cultures constituted the case group, while those who had febrile neutropenia without any growth in their cultures and who did not have any documented infections formed the control group. Blood culture bottles were incubated in the Bact ALERT 3D system (bioMerieux, France), identification and susceptibility testing were performed using an automated broth microdilution method (VITEK 2, bioMerieux), and CD64 expression analysis was performed by the flow cytometry method. C-reactive protein (CRP) was measured by turbidimetric methods (Biosystems, Spain) and erythrocyte sedimentation rate (ESR) was measured by the Wintrobe method. Results: In total, we prospectively evaluated 31 febrile episodes. The case group consisted of 17 patients while the control group included 14 patients. CD64 was found on neutrophils of the case group patients with a mean count of 8006 molecules/cell and of control group with a mean count of 2786 molecules/cell. CD64 levels of the case group were significantly higher than those of the control group (p=0.005). In the differentiation of the case group from the control group, a 2500 cut-off value for CD64 had significant [AUC=0.792 (0.619-0.965)] predictive value (p=0.001). In the prediction of patients with a 2500 cut-off value for CD64, sensitivity was 94.1%, positive predictive value was 76.2%, specificity was 64.3%, and negative predictive value was 90.0%. CRP levels and ESR values did not differ significantly between the groups (p=0.005). Conclusion: Neutrophil CD64 expression could be a good predictor as an immune parameter with high sensitivity and a negative predictive value for bacteremia in febrile neutropenic patients. |
| first_indexed | 2024-04-10T14:09:47Z |
| format | Article |
| id | doaj.art-49b602036d674ba3ba0f4a134d5126c2 |
| institution | Directory Open Access Journal |
| issn | 1308-5263 |
| language | English |
| last_indexed | 2024-04-10T14:09:47Z |
| publishDate | 2017-05-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Turkish Journal of Hematology |
| spelling | doaj.art-49b602036d674ba3ba0f4a134d5126c22023-02-15T16:09:47ZengGalenos Publishing HouseTurkish Journal of Hematology1308-52632017-05-0134216717310.4274/tjh.2016.0123TJH-82246Could Neutrophil CD64 Expression Be Used as a Diagnostic Parameter of Bacteremia in Patients with Febrile Neutropenia?Nur Efe İris0Taner Yıldırmak1Habip Gedik2Funda Şimşek3Demet Aydın4Naciye Demirel5Osman Yokuş6İstanbul Bilim University Faculty of Medicine, Department of Infectious Diseases and Clinical Microbiology, İstanbul, TurkeyOkmeydanı Training and Research Hospital, Clinic of Infectious Diseases and Clinical Microbiology, İstanbul, TurkeyOkmeydanı Training and Research Hospital, Clinic of Infectious Diseases and Clinical Microbiology, İstanbul, TurkeyOkmeydanı Training and Research Hospital, Clinic of Infectious Diseases and Clinical Microbiology, İstanbul, TurkeyOkmeydanı Training and Research Hospital, Clinic of Hematology, İstanbul, TurkeyOkmeydanı Training and Research Hospital, Clinic of Hematology, İstanbul, TurkeyOkmeydanı Training and Research Hospital, Clinic of Hematology, İstanbul, TurkeyObjective: The aim of this study is to investigate if neutrophil CD64 expression in febrile neutropenia patients could be used as an early indicator of bacteremia. Materials and Methods: All consecutive patients older than 18 years of age who had developed febrile neutropenia episodes due to hematological malignancies were included in the study. Those patients who had significant growth in their blood cultures constituted the case group, while those who had febrile neutropenia without any growth in their cultures and who did not have any documented infections formed the control group. Blood culture bottles were incubated in the Bact ALERT 3D system (bioMerieux, France), identification and susceptibility testing were performed using an automated broth microdilution method (VITEK 2, bioMerieux), and CD64 expression analysis was performed by the flow cytometry method. C-reactive protein (CRP) was measured by turbidimetric methods (Biosystems, Spain) and erythrocyte sedimentation rate (ESR) was measured by the Wintrobe method. Results: In total, we prospectively evaluated 31 febrile episodes. The case group consisted of 17 patients while the control group included 14 patients. CD64 was found on neutrophils of the case group patients with a mean count of 8006 molecules/cell and of control group with a mean count of 2786 molecules/cell. CD64 levels of the case group were significantly higher than those of the control group (p=0.005). In the differentiation of the case group from the control group, a 2500 cut-off value for CD64 had significant [AUC=0.792 (0.619-0.965)] predictive value (p=0.001). In the prediction of patients with a 2500 cut-off value for CD64, sensitivity was 94.1%, positive predictive value was 76.2%, specificity was 64.3%, and negative predictive value was 90.0%. CRP levels and ESR values did not differ significantly between the groups (p=0.005). Conclusion: Neutrophil CD64 expression could be a good predictor as an immune parameter with high sensitivity and a negative predictive value for bacteremia in febrile neutropenic patients.https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-82246neutrophilcd64bacteremiafebril neutropeniadiagnostic parameter |
| spellingShingle | Nur Efe İris Taner Yıldırmak Habip Gedik Funda Şimşek Demet Aydın Naciye Demirel Osman Yokuş Could Neutrophil CD64 Expression Be Used as a Diagnostic Parameter of Bacteremia in Patients with Febrile Neutropenia? Turkish Journal of Hematology neutrophil cd64 bacteremia febril neutropenia diagnostic parameter |
| title | Could Neutrophil CD64 Expression Be Used as a Diagnostic Parameter of Bacteremia in Patients with Febrile Neutropenia? |
| title_full | Could Neutrophil CD64 Expression Be Used as a Diagnostic Parameter of Bacteremia in Patients with Febrile Neutropenia? |
| title_fullStr | Could Neutrophil CD64 Expression Be Used as a Diagnostic Parameter of Bacteremia in Patients with Febrile Neutropenia? |
| title_full_unstemmed | Could Neutrophil CD64 Expression Be Used as a Diagnostic Parameter of Bacteremia in Patients with Febrile Neutropenia? |
| title_short | Could Neutrophil CD64 Expression Be Used as a Diagnostic Parameter of Bacteremia in Patients with Febrile Neutropenia? |
| title_sort | could neutrophil cd64 expression be used as a diagnostic parameter of bacteremia in patients with febrile neutropenia |
| topic | neutrophil cd64 bacteremia febril neutropenia diagnostic parameter |
| url | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-82246 |
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