Development and Characterization of 32 Microsatellite Loci in <i>Genipa americana</i> (Rubiaceae)
Premise of the study: Microsatellite primers were developed for the tree species <i>Genipa americana</i> (Rubiaceae) for further population genetic studies. Methods and Results: We identified 144 clones containing 65 repeat motifs from a genomic library enriched for (CT)<sub>8<...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2014-02-01
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| Series: | Applications in Plant Sciences |
| Subjects: | |
| Online Access: | http://www.bioone.org/doi/full/10.3732/apps.1300084 |
| _version_ | 1828522077543464960 |
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| author | Ricardo O. Manoel Miguel L. M. Freitas Mariana A. Barreto Mário L. T. Moraes Anete P. Souza Alexandre M. Sebbenn |
| author_facet | Ricardo O. Manoel Miguel L. M. Freitas Mariana A. Barreto Mário L. T. Moraes Anete P. Souza Alexandre M. Sebbenn |
| author_sort | Ricardo O. Manoel |
| collection | DOAJ |
| description | Premise of the study: Microsatellite primers were developed for the tree species <i>Genipa americana</i> (Rubiaceae) for further population genetic studies.
Methods and Results: We identified 144 clones containing 65 repeat motifs from a genomic library enriched for (CT)<sub>8</sub> and (GT)<sub>8</sub> motifs. Primer pairs were developed for 32 microsatellite loci and validated in 40 individuals of two natural <i>G. americana</i> populations. Seventeen loci were polymorphic, revealing from three to seven alleles per locus. The observed and expected heterozygosities ranged from 0.24 to 1.00 and from 0.22 to 0.78, respectively.
Conclusions: The 17 primers identified as polymorphic loci are suitable to study the genetic diversity and structure, mating system, and gene flow in <i>G. americana</i>. |
| first_indexed | 2024-12-11T20:00:59Z |
| format | Article |
| id | doaj.art-49b72ae7cc4346b3851b112ec478269c |
| institution | Directory Open Access Journal |
| issn | 2168-0450 |
| language | English |
| last_indexed | 2024-12-11T20:00:59Z |
| publishDate | 2014-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | Applications in Plant Sciences |
| spelling | doaj.art-49b72ae7cc4346b3851b112ec478269c2022-12-22T00:52:31ZengWileyApplications in Plant Sciences2168-04502014-02-0123130008410.3732/apps.1300084Development and Characterization of 32 Microsatellite Loci in <i>Genipa americana</i> (Rubiaceae)Ricardo O. Manoel0Miguel L. M. Freitas1Mariana A. Barreto2Mário L. T. Moraes3Anete P. Souza4Alexandre M. Sebbenn5Faculdade de Engenharia de Ilha Solteira, Universidade Estadual Paulista (UNESP), Av. Brasil Centro 56, CP 31, Ilha Solteira, São Paulo 15385-000, BrazilInstituto Florestal de São Paulo, CP 1322, São Paulo, São Paulo 01059-970, BrazilCentro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas (UNICAMP), CP 6010, Campinas, São Paulo 13083-970, BrazilFaculdade de Engenharia de Ilha Solteira, Universidade Estadual Paulista (UNESP), Av. Brasil Centro 56, CP 31, Ilha Solteira, São Paulo 15385-000, BrazilCentro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas (UNICAMP), CP 6010, Campinas, São Paulo 13083-970, BrazilInstituto Florestal de São Paulo, CP 1322, São Paulo, São Paulo 01059-970, BrazilPremise of the study: Microsatellite primers were developed for the tree species <i>Genipa americana</i> (Rubiaceae) for further population genetic studies. Methods and Results: We identified 144 clones containing 65 repeat motifs from a genomic library enriched for (CT)<sub>8</sub> and (GT)<sub>8</sub> motifs. Primer pairs were developed for 32 microsatellite loci and validated in 40 individuals of two natural <i>G. americana</i> populations. Seventeen loci were polymorphic, revealing from three to seven alleles per locus. The observed and expected heterozygosities ranged from 0.24 to 1.00 and from 0.22 to 0.78, respectively. Conclusions: The 17 primers identified as polymorphic loci are suitable to study the genetic diversity and structure, mating system, and gene flow in <i>G. americana</i>.http://www.bioone.org/doi/full/10.3732/apps.1300084conservation geneticsGenipa americanamicrosatellite markerspopulation geneticsRubiaceaetree species |
| spellingShingle | Ricardo O. Manoel Miguel L. M. Freitas Mariana A. Barreto Mário L. T. Moraes Anete P. Souza Alexandre M. Sebbenn Development and Characterization of 32 Microsatellite Loci in <i>Genipa americana</i> (Rubiaceae) Applications in Plant Sciences conservation genetics Genipa americana microsatellite markers population genetics Rubiaceae tree species |
| title | Development and Characterization of 32 Microsatellite Loci in <i>Genipa americana</i> (Rubiaceae) |
| title_full | Development and Characterization of 32 Microsatellite Loci in <i>Genipa americana</i> (Rubiaceae) |
| title_fullStr | Development and Characterization of 32 Microsatellite Loci in <i>Genipa americana</i> (Rubiaceae) |
| title_full_unstemmed | Development and Characterization of 32 Microsatellite Loci in <i>Genipa americana</i> (Rubiaceae) |
| title_short | Development and Characterization of 32 Microsatellite Loci in <i>Genipa americana</i> (Rubiaceae) |
| title_sort | development and characterization of 32 microsatellite loci in i genipa americana i rubiaceae |
| topic | conservation genetics Genipa americana microsatellite markers population genetics Rubiaceae tree species |
| url | http://www.bioone.org/doi/full/10.3732/apps.1300084 |
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