Advanced technologies for molecular diagnosis of cancer: State of pre-clinical tumor-derived exosome liquid biopsies
Exosomes are membrane-defined extracellular vesicles (EVs) approximately 40–160 nm in diameter that are found in all body fluids including blood, urine, and saliva. They act as important vehicles for intercellular communication between both local and distant cells and can serve as circulating bioma...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-02-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006422003362 |
| _version_ | 1797938035789135872 |
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| author | Lin Li Lili Zhang Katelynn C. Montgomery Li Jiang Christopher J. Lyon Tony Y. Hu |
| author_facet | Lin Li Lili Zhang Katelynn C. Montgomery Li Jiang Christopher J. Lyon Tony Y. Hu |
| author_sort | Lin Li |
| collection | DOAJ |
| description | Exosomes are membrane-defined extracellular vesicles (EVs) approximately 40–160 nm in diameter that are found in all body fluids including blood, urine, and saliva. They act as important vehicles for intercellular communication between both local and distant cells and can serve as circulating biomarkers for disease diagnosis and prognosis. Exosomes play a key role in tumor metastasis, are abundant in biofluids, and stabilize biomarkers they carry, and thus can improve cancer detection, treatment monitoring, and cancer staging/prognosis. Despite their clinical potential, lack of sensitive/specific biomarkers and sensitive isolation/enrichment and analytical technologies has posed a barrier to clinical translation of exosomes. This review presents a critical overview of technologies now being used to detect tumor-derived exosome (TDE) biomarkers in clinical specimens that have potential for clinical translation. |
| first_indexed | 2024-04-10T18:54:30Z |
| format | Article |
| id | doaj.art-49b7e1b6c0c448c2b2addb50a66d7f67 |
| institution | Directory Open Access Journal |
| issn | 2590-0064 |
| language | English |
| last_indexed | 2024-04-10T18:54:30Z |
| publishDate | 2023-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj.art-49b7e1b6c0c448c2b2addb50a66d7f672023-02-01T04:27:26ZengElsevierMaterials Today Bio2590-00642023-02-0118100538Advanced technologies for molecular diagnosis of cancer: State of pre-clinical tumor-derived exosome liquid biopsiesLin Li0Lili Zhang1Katelynn C. Montgomery2Li Jiang3Christopher J. Lyon4Tony Y. Hu5Department of Laboratory Medicine and Sichuan Provincial Key Laboratory for Human Disease Gene Study, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu, China; Center for Cellular and Molecular Diagnostics, Department of Biochemistry and Molecular Biology, School of Medicine, Tulane University, New Orleans, LA, USACenter for Cellular and Molecular Diagnostics, Department of Biochemistry and Molecular Biology, School of Medicine, Tulane University, New Orleans, LA, USA; HCA Florida Healthcare Westside/Northwest Hospital Internal Medicine, Plantation, Florida, USADepartment of Biomedical Engineering, School of Science and Engineering, Tulane University, New Orleans, LA, USADepartment of Laboratory Medicine and Sichuan Provincial Key Laboratory for Human Disease Gene Study, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu, ChinaCenter for Cellular and Molecular Diagnostics, Department of Biochemistry and Molecular Biology, School of Medicine, Tulane University, New Orleans, LA, USACenter for Cellular and Molecular Diagnostics, Department of Biochemistry and Molecular Biology, School of Medicine, Tulane University, New Orleans, LA, USA; Department of Biomedical Engineering, School of Science and Engineering, Tulane University, New Orleans, LA, USA; Corresponding author. Center for Cellular and Molecular Diagnostics, Department of Biochemistry and Molecular Biology, School of Medicine, Tulane University, New Orleans, LA, USA.Exosomes are membrane-defined extracellular vesicles (EVs) approximately 40–160 nm in diameter that are found in all body fluids including blood, urine, and saliva. They act as important vehicles for intercellular communication between both local and distant cells and can serve as circulating biomarkers for disease diagnosis and prognosis. Exosomes play a key role in tumor metastasis, are abundant in biofluids, and stabilize biomarkers they carry, and thus can improve cancer detection, treatment monitoring, and cancer staging/prognosis. Despite their clinical potential, lack of sensitive/specific biomarkers and sensitive isolation/enrichment and analytical technologies has posed a barrier to clinical translation of exosomes. This review presents a critical overview of technologies now being used to detect tumor-derived exosome (TDE) biomarkers in clinical specimens that have potential for clinical translation.http://www.sciencedirect.com/science/article/pii/S2590006422003362CancerMolecular diagnosisExosomeIsolationEnrichmentLaboratory developed tests |
| spellingShingle | Lin Li Lili Zhang Katelynn C. Montgomery Li Jiang Christopher J. Lyon Tony Y. Hu Advanced technologies for molecular diagnosis of cancer: State of pre-clinical tumor-derived exosome liquid biopsies Materials Today Bio Cancer Molecular diagnosis Exosome Isolation Enrichment Laboratory developed tests |
| title | Advanced technologies for molecular diagnosis of cancer: State of pre-clinical tumor-derived exosome liquid biopsies |
| title_full | Advanced technologies for molecular diagnosis of cancer: State of pre-clinical tumor-derived exosome liquid biopsies |
| title_fullStr | Advanced technologies for molecular diagnosis of cancer: State of pre-clinical tumor-derived exosome liquid biopsies |
| title_full_unstemmed | Advanced technologies for molecular diagnosis of cancer: State of pre-clinical tumor-derived exosome liquid biopsies |
| title_short | Advanced technologies for molecular diagnosis of cancer: State of pre-clinical tumor-derived exosome liquid biopsies |
| title_sort | advanced technologies for molecular diagnosis of cancer state of pre clinical tumor derived exosome liquid biopsies |
| topic | Cancer Molecular diagnosis Exosome Isolation Enrichment Laboratory developed tests |
| url | http://www.sciencedirect.com/science/article/pii/S2590006422003362 |
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