microRNA-140 Regulates PDGFRα and Is Involved in Adipocyte Differentiation
In recent years, the studies of the role of microRNAs in adipogenesis and adipocyte development and the corresponding molecular mechanisms have received great attention. In this work, we investigated the function of miR-140 in the process of adipogenesis and the molecular pathways involved, and we f...
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| Format: | Article |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Molecular Biosciences |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2022.907148/full |
| _version_ | 1818157173111259136 |
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| author | Yi Yan Jiahui Yuan Xiaomao Luo Xiuju Yu Jiayin Lu Wei Hou Xiaoyan He Liping Zhang Jing Cao Haidong Wang |
| author_facet | Yi Yan Jiahui Yuan Xiaomao Luo Xiuju Yu Jiayin Lu Wei Hou Xiaoyan He Liping Zhang Jing Cao Haidong Wang |
| author_sort | Yi Yan |
| collection | DOAJ |
| description | In recent years, the studies of the role of microRNAs in adipogenesis and adipocyte development and the corresponding molecular mechanisms have received great attention. In this work, we investigated the function of miR-140 in the process of adipogenesis and the molecular pathways involved, and we found that adipogenic treatment promoted the miR-140-5p RNA level in preadipocytes. Over-expression of miR-140-5p in preadipocytes accelerated lipogenesis along with adipogenic differentiation by transcriptional modulation of adipogenesis-linked genes. Meanwhile, silencing endogenous miR-140-5p dampened adipogenesis. Platelet-derived growth factor receptor alpha (PDGFRα) was shown to be a miR-140-5p target gene. miR-140-5p over-expression in preadipocyte 3T3-L1 diminished PDGFRα expression, but silencing of miR-140-5p augmented it. In addition, over-expression of PDGFRα suppressed adipogenic differentiation and lipogenesis, while its knockdown enhanced these biological processes of preadipocyte 3T3-L1. Altogether, our current findings reveal that miR-140-5p induces lipogenesis and adipogenic differentiation in 3T3-L1 cells by targeting PDGFRα, therefore regulating adipogenesis. Our research provides molecular targets and a theoretical basis for the treatment of obesity-related metabolic diseases. |
| first_indexed | 2024-12-11T15:09:59Z |
| format | Article |
| id | doaj.art-49b914691e9b4fbb906044451e0e3d25 |
| institution | Directory Open Access Journal |
| issn | 2296-889X |
| language | English |
| last_indexed | 2024-12-11T15:09:59Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-49b914691e9b4fbb906044451e0e3d252022-12-22T01:00:46ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2022-06-01910.3389/fmolb.2022.907148907148microRNA-140 Regulates PDGFRα and Is Involved in Adipocyte DifferentiationYi Yan0Jiahui Yuan1Xiaomao Luo2Xiuju Yu3Jiayin Lu4Wei Hou5Xiaoyan He6Liping Zhang7Jing Cao8Haidong Wang9College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Jinzhong, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Jinzhong, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Jinzhong, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Jinzhong, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Jinzhong, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Jinzhong, ChinaDepartment of Medicine, Nephrology Division, Baylor College of Medicine, Houston, GA, United StatesDepartment of Animal Husbandry and Veterinary Medicine, Beijing Vocational College of Agriculture, Beijing, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Jinzhong, ChinaIn recent years, the studies of the role of microRNAs in adipogenesis and adipocyte development and the corresponding molecular mechanisms have received great attention. In this work, we investigated the function of miR-140 in the process of adipogenesis and the molecular pathways involved, and we found that adipogenic treatment promoted the miR-140-5p RNA level in preadipocytes. Over-expression of miR-140-5p in preadipocytes accelerated lipogenesis along with adipogenic differentiation by transcriptional modulation of adipogenesis-linked genes. Meanwhile, silencing endogenous miR-140-5p dampened adipogenesis. Platelet-derived growth factor receptor alpha (PDGFRα) was shown to be a miR-140-5p target gene. miR-140-5p over-expression in preadipocyte 3T3-L1 diminished PDGFRα expression, but silencing of miR-140-5p augmented it. In addition, over-expression of PDGFRα suppressed adipogenic differentiation and lipogenesis, while its knockdown enhanced these biological processes of preadipocyte 3T3-L1. Altogether, our current findings reveal that miR-140-5p induces lipogenesis and adipogenic differentiation in 3T3-L1 cells by targeting PDGFRα, therefore regulating adipogenesis. Our research provides molecular targets and a theoretical basis for the treatment of obesity-related metabolic diseases.https://www.frontiersin.org/articles/10.3389/fmolb.2022.907148/fullmiR-140-5padipocyte3T3-L1 cellsdifferentiationadipogenesis |
| spellingShingle | Yi Yan Jiahui Yuan Xiaomao Luo Xiuju Yu Jiayin Lu Wei Hou Xiaoyan He Liping Zhang Jing Cao Haidong Wang microRNA-140 Regulates PDGFRα and Is Involved in Adipocyte Differentiation Frontiers in Molecular Biosciences miR-140-5p adipocyte 3T3-L1 cells differentiation adipogenesis |
| title | microRNA-140 Regulates PDGFRα and Is Involved in Adipocyte Differentiation |
| title_full | microRNA-140 Regulates PDGFRα and Is Involved in Adipocyte Differentiation |
| title_fullStr | microRNA-140 Regulates PDGFRα and Is Involved in Adipocyte Differentiation |
| title_full_unstemmed | microRNA-140 Regulates PDGFRα and Is Involved in Adipocyte Differentiation |
| title_short | microRNA-140 Regulates PDGFRα and Is Involved in Adipocyte Differentiation |
| title_sort | microrna 140 regulates pdgfrα and is involved in adipocyte differentiation |
| topic | miR-140-5p adipocyte 3T3-L1 cells differentiation adipogenesis |
| url | https://www.frontiersin.org/articles/10.3389/fmolb.2022.907148/full |
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