Pharmacological Validation for the Folklore Use of <i>Ipomoea nil</i> against Asthma: In Vivo and In Vitro Evaluation
Oxidative stress is the key factor that strengthens free radical generation which stimulates lung inflammation. The aim was to explore antioxidant, bronchodilatory along with anti-asthmatic potential of folkloric plants and the aqueous methanolic crude extract of <i>Ipomoea nil</i> (<...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-07-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/27/14/4653 |
| _version_ | 1797416644088168448 |
|---|---|
| author | Taha Alqahtani Sajida Parveen Yahia Alghazwani Hanan M. Alharbi Reem M. Gahtani Nadia Hussain Kashif ur Rehman Musaddique Hussain |
| author_facet | Taha Alqahtani Sajida Parveen Yahia Alghazwani Hanan M. Alharbi Reem M. Gahtani Nadia Hussain Kashif ur Rehman Musaddique Hussain |
| author_sort | Taha Alqahtani |
| collection | DOAJ |
| description | Oxidative stress is the key factor that strengthens free radical generation which stimulates lung inflammation. The aim was to explore antioxidant, bronchodilatory along with anti-asthmatic potential of folkloric plants and the aqueous methanolic crude extract of <i>Ipomoea nil</i> (<i>In.Cr</i>) seeds which may demonstrate as more potent, economically affordable, having an improved antioxidant profile and providing evidence as exclusive therapeutic agents in respiratory pharmacology. In vitro antioxidant temperament was executed by DPPH, TFC, TPC and HPLC in addition to enzyme inhibition (cholinesterase) analysis; a bronchodilator assay on rabbit’s trachea as well as in vivo OVA-induced allergic asthmatic activity was performed on mice. In vitro analysis of 1,1-Diphenyl-2-picrylhydrazyl radical (DPPH) expressed as % inhibition 86.28 ± 0.25 with IC<sub>50</sub> 17.22 ± 0.56 mol/L, TPC 115.5 ± 1.02 mg GAE/g of dry sample, TFC 50.44 ± 1.06 mg QE/g dry weight of sample, inhibition in cholinesterase levels for acetyl and butyryl with IC<sub>50</sub> (0.60 ± 0.67 and 1.5 ± 0.04 mol/L) in comparison with standard 0.06 ± 0.002 and 0.30 ± 0.003, respectively, while HPLC characterization of <i>In.Cr</i> confirmed the existence with identification as well as quantification of various polyphenolics and flavonoids i.e., gallic acid, vanillic acid, chlorogenic acid, quercetin, kaempferol and others. However, oral gavage of <i>In.Cr</i> at different doses in rabbits showed a better brochodilation profile as compared to carbachol and K<sup>+</sup>-induced bronchospasm. More significant (<i>p</i> < 0.01) reduction in OVA-induced allergic hyper-responses i.e., inflammatory cells grade, antibody IgE as well as altered IFN-α in airways were observed at three different doses of <i>In.Cr</i>. It can be concluded that sound mechanistic basis i.e., the existence of antioxidants: various phenolic and flavonoids, calcium antagonist(s) as well as enzymes’ inhibition profile, validates folkloric consumptions of this traditionally used plant to treat ailments of respiration. |
| first_indexed | 2024-03-09T06:06:11Z |
| format | Article |
| id | doaj.art-49b9b55126734da6a1a7e5ee338ed31c |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T06:06:11Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-49b9b55126734da6a1a7e5ee338ed31c2023-12-03T12:02:42ZengMDPI AGMolecules1420-30492022-07-012714465310.3390/molecules27144653Pharmacological Validation for the Folklore Use of <i>Ipomoea nil</i> against Asthma: In Vivo and In Vitro EvaluationTaha Alqahtani0Sajida Parveen1Yahia Alghazwani2Hanan M. Alharbi3Reem M. Gahtani4Nadia Hussain5Kashif ur Rehman6Musaddique Hussain7Department of Pharmacology, College of Pharmacy, King Khalid University, Abha 62529, Saudi ArabiaFaculty of Pharmacy, TheIslamia University of Bahawalpur, Bahawalpur 63100, PakistanDepartment of Pharmacology, College of Pharmacy, King Khalid University, Abha 62529, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, Umm A-Qura University, Makkah 21955, Saudi ArabiaDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, Abha 61421, Saudi ArabiaDepartment of Pharmaceutical Sciences, College of Pharmacy, Al Ain University, Al Ain 64141, United Arab EmiratesFaculty of Pharmacy, TheIslamia University of Bahawalpur, Bahawalpur 63100, PakistanFaculty of Pharmacy, TheIslamia University of Bahawalpur, Bahawalpur 63100, PakistanOxidative stress is the key factor that strengthens free radical generation which stimulates lung inflammation. The aim was to explore antioxidant, bronchodilatory along with anti-asthmatic potential of folkloric plants and the aqueous methanolic crude extract of <i>Ipomoea nil</i> (<i>In.Cr</i>) seeds which may demonstrate as more potent, economically affordable, having an improved antioxidant profile and providing evidence as exclusive therapeutic agents in respiratory pharmacology. In vitro antioxidant temperament was executed by DPPH, TFC, TPC and HPLC in addition to enzyme inhibition (cholinesterase) analysis; a bronchodilator assay on rabbit’s trachea as well as in vivo OVA-induced allergic asthmatic activity was performed on mice. In vitro analysis of 1,1-Diphenyl-2-picrylhydrazyl radical (DPPH) expressed as % inhibition 86.28 ± 0.25 with IC<sub>50</sub> 17.22 ± 0.56 mol/L, TPC 115.5 ± 1.02 mg GAE/g of dry sample, TFC 50.44 ± 1.06 mg QE/g dry weight of sample, inhibition in cholinesterase levels for acetyl and butyryl with IC<sub>50</sub> (0.60 ± 0.67 and 1.5 ± 0.04 mol/L) in comparison with standard 0.06 ± 0.002 and 0.30 ± 0.003, respectively, while HPLC characterization of <i>In.Cr</i> confirmed the existence with identification as well as quantification of various polyphenolics and flavonoids i.e., gallic acid, vanillic acid, chlorogenic acid, quercetin, kaempferol and others. However, oral gavage of <i>In.Cr</i> at different doses in rabbits showed a better brochodilation profile as compared to carbachol and K<sup>+</sup>-induced bronchospasm. More significant (<i>p</i> < 0.01) reduction in OVA-induced allergic hyper-responses i.e., inflammatory cells grade, antibody IgE as well as altered IFN-α in airways were observed at three different doses of <i>In.Cr</i>. It can be concluded that sound mechanistic basis i.e., the existence of antioxidants: various phenolic and flavonoids, calcium antagonist(s) as well as enzymes’ inhibition profile, validates folkloric consumptions of this traditionally used plant to treat ailments of respiration.https://www.mdpi.com/1420-3049/27/14/4653<i>Ipomoea</i> <i>nil</i> seedsbronchodilatoranti-asthmaticIgE antibodyinflammatory cellshyper-responsive |
| spellingShingle | Taha Alqahtani Sajida Parveen Yahia Alghazwani Hanan M. Alharbi Reem M. Gahtani Nadia Hussain Kashif ur Rehman Musaddique Hussain Pharmacological Validation for the Folklore Use of <i>Ipomoea nil</i> against Asthma: In Vivo and In Vitro Evaluation Molecules <i>Ipomoea</i> <i>nil</i> seeds bronchodilator anti-asthmatic IgE antibody inflammatory cells hyper-responsive |
| title | Pharmacological Validation for the Folklore Use of <i>Ipomoea nil</i> against Asthma: In Vivo and In Vitro Evaluation |
| title_full | Pharmacological Validation for the Folklore Use of <i>Ipomoea nil</i> against Asthma: In Vivo and In Vitro Evaluation |
| title_fullStr | Pharmacological Validation for the Folklore Use of <i>Ipomoea nil</i> against Asthma: In Vivo and In Vitro Evaluation |
| title_full_unstemmed | Pharmacological Validation for the Folklore Use of <i>Ipomoea nil</i> against Asthma: In Vivo and In Vitro Evaluation |
| title_short | Pharmacological Validation for the Folklore Use of <i>Ipomoea nil</i> against Asthma: In Vivo and In Vitro Evaluation |
| title_sort | pharmacological validation for the folklore use of i ipomoea nil i against asthma in vivo and in vitro evaluation |
| topic | <i>Ipomoea</i> <i>nil</i> seeds bronchodilator anti-asthmatic IgE antibody inflammatory cells hyper-responsive |
| url | https://www.mdpi.com/1420-3049/27/14/4653 |
| work_keys_str_mv | AT tahaalqahtani pharmacologicalvalidationforthefolkloreuseofiipomoeaniliagainstasthmainvivoandinvitroevaluation AT sajidaparveen pharmacologicalvalidationforthefolkloreuseofiipomoeaniliagainstasthmainvivoandinvitroevaluation AT yahiaalghazwani pharmacologicalvalidationforthefolkloreuseofiipomoeaniliagainstasthmainvivoandinvitroevaluation AT hananmalharbi pharmacologicalvalidationforthefolkloreuseofiipomoeaniliagainstasthmainvivoandinvitroevaluation AT reemmgahtani pharmacologicalvalidationforthefolkloreuseofiipomoeaniliagainstasthmainvivoandinvitroevaluation AT nadiahussain pharmacologicalvalidationforthefolkloreuseofiipomoeaniliagainstasthmainvivoandinvitroevaluation AT kashifurrehman pharmacologicalvalidationforthefolkloreuseofiipomoeaniliagainstasthmainvivoandinvitroevaluation AT musaddiquehussain pharmacologicalvalidationforthefolkloreuseofiipomoeaniliagainstasthmainvivoandinvitroevaluation |