Cigarette smoke extract induces the senescence of endothelial progenitor cells by upregulating p300
Introduction Endothelial progenitor cells (EPCs) are the main source of endothelial cells. The senescence of EPCs is involved in the pathogenesis of chronic obstructive pulmonary disease (COPD). Cigarette smoke extract (CSE) can directly induce the dysfunction and increased expression of senescence-...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
European Publishing
2023-10-01
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| Series: | Tobacco Induced Diseases |
| Subjects: | |
| Online Access: | http://www.tobaccoinduceddiseases.org/Cigarette-smoke-extract-induces-the-senescence-of-endothelial-nprogenitor-cells-by,170581,0,2.html |
| _version_ | 1797390072199249920 |
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| author | Guibin Liang Zhihui He Huaihuai Peng Menghao Zeng Xuefeng Zhang |
| author_facet | Guibin Liang Zhihui He Huaihuai Peng Menghao Zeng Xuefeng Zhang |
| author_sort | Guibin Liang |
| collection | DOAJ |
| description | Introduction
Endothelial progenitor cells (EPCs) are the main source of endothelial
cells. The senescence of EPCs is involved in the pathogenesis of chronic obstructive
pulmonary disease (COPD). Cigarette smoke extract (CSE) can directly induce
the dysfunction and increased expression of senescence-related markers in EPCs
cultured in vitro. Histone acetyltransferase p300 is a transcriptional activator, and
its changes can lead to cell senescence. The present study investigated whether
CSE can induce the senescence of EPCs by upregulating p300.
Methods
EPCs were isolated from bone marrow of C57BL/6J mice by density
gradient centrifugation. The p300 inhibitor C646 and agonist CTPB were used
to interfere with EPCs, cell cycle and apoptosis were detected by flow cytometry,
the proportion of senile cells was counted by β-galactosidase staining, the protein
expression of p300, H4K12, Cyclin D1, TERT and Ki67 were detected by western
blot.
Results
Compared with the control group, the cell cycle of CSE group and CTPB
group were blocked, the apoptosis rate and early apoptosis rate were increased,
the proportion of senile cells counted by β-galactosidase staining was increased,
the expression of p300 and H4K12 protein were increased, the expression of
Cyclin D1, TERT and Ki67 protein were decreased. C646 could partly alleviate
the damages caused by CSE.
Conclusions
CSE may promote the apoptosis and senescence of EPCs by
upregulating the expression of p300 and H4K12 protein, thus preventing the
transition of EPCs from G1 phase to S phase, affecting telomerase synthesis, and
reducing EPCs proliferation. |
| first_indexed | 2024-03-08T23:06:18Z |
| format | Article |
| id | doaj.art-49c10c3e1da34908bb4f0f4cc19808ed |
| institution | Directory Open Access Journal |
| issn | 1617-9625 |
| language | English |
| last_indexed | 2024-03-08T23:06:18Z |
| publishDate | 2023-10-01 |
| publisher | European Publishing |
| record_format | Article |
| series | Tobacco Induced Diseases |
| spelling | doaj.art-49c10c3e1da34908bb4f0f4cc19808ed2023-12-15T12:41:24ZengEuropean PublishingTobacco Induced Diseases1617-96252023-10-0121October1910.18332/tid/170581170581Cigarette smoke extract induces the senescence of endothelial progenitor cells by upregulating p300Guibin Liang0Zhihui He1https://orcid.org/0000-0001-5672-9691Huaihuai Peng2Menghao Zeng3Xuefeng Zhang4Department of Critical Care Medicine, the Third Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Critical Care Medicine, the Third Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Critical Care Medicine, the Second Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Critical Care Medicine, the Third Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Critical Care Medicine, the Third Xiangya Hospital, Central South University, Changsha, ChinaIntroduction Endothelial progenitor cells (EPCs) are the main source of endothelial cells. The senescence of EPCs is involved in the pathogenesis of chronic obstructive pulmonary disease (COPD). Cigarette smoke extract (CSE) can directly induce the dysfunction and increased expression of senescence-related markers in EPCs cultured in vitro. Histone acetyltransferase p300 is a transcriptional activator, and its changes can lead to cell senescence. The present study investigated whether CSE can induce the senescence of EPCs by upregulating p300. Methods EPCs were isolated from bone marrow of C57BL/6J mice by density gradient centrifugation. The p300 inhibitor C646 and agonist CTPB were used to interfere with EPCs, cell cycle and apoptosis were detected by flow cytometry, the proportion of senile cells was counted by β-galactosidase staining, the protein expression of p300, H4K12, Cyclin D1, TERT and Ki67 were detected by western blot. Results Compared with the control group, the cell cycle of CSE group and CTPB group were blocked, the apoptosis rate and early apoptosis rate were increased, the proportion of senile cells counted by β-galactosidase staining was increased, the expression of p300 and H4K12 protein were increased, the expression of Cyclin D1, TERT and Ki67 protein were decreased. C646 could partly alleviate the damages caused by CSE. Conclusions CSE may promote the apoptosis and senescence of EPCs by upregulating the expression of p300 and H4K12 protein, thus preventing the transition of EPCs from G1 phase to S phase, affecting telomerase synthesis, and reducing EPCs proliferation.http://www.tobaccoinduceddiseases.org/Cigarette-smoke-extract-induces-the-senescence-of-endothelial-nprogenitor-cells-by,170581,0,2.htmlp300cigarette smoke extractendothelial progenitor cellssenescence |
| spellingShingle | Guibin Liang Zhihui He Huaihuai Peng Menghao Zeng Xuefeng Zhang Cigarette smoke extract induces the senescence of endothelial progenitor cells by upregulating p300 Tobacco Induced Diseases p300 cigarette smoke extract endothelial progenitor cells senescence |
| title | Cigarette smoke extract induces the senescence of endothelial
progenitor cells by upregulating p300 |
| title_full | Cigarette smoke extract induces the senescence of endothelial
progenitor cells by upregulating p300 |
| title_fullStr | Cigarette smoke extract induces the senescence of endothelial
progenitor cells by upregulating p300 |
| title_full_unstemmed | Cigarette smoke extract induces the senescence of endothelial
progenitor cells by upregulating p300 |
| title_short | Cigarette smoke extract induces the senescence of endothelial
progenitor cells by upregulating p300 |
| title_sort | cigarette smoke extract induces the senescence of endothelial progenitor cells by upregulating p300 |
| topic | p300 cigarette smoke extract endothelial progenitor cells senescence |
| url | http://www.tobaccoinduceddiseases.org/Cigarette-smoke-extract-induces-the-senescence-of-endothelial-nprogenitor-cells-by,170581,0,2.html |
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