Extract from the <i>Coriolus versicolor</i> Fungus as an Anti-Inflammatory Agent with Cytotoxic Properties against Endothelial Cells and Breast Cancer Cells
Chronic inflammation is a well-recognised tumour-enabling component, which includes bioactive molecules from cells infiltrating the tumour microenvironment and increases the risk of cancer progression. Since long-term use of the currently available anti-inflammatory drugs used in cancer therapy caus...
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MDPI AG
2020-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/23/9063 |
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| author | Tomasz Jędrzejewski Justyna Sobocińska Małgorzata Pawlikowska Artur Dzialuk Sylwia Wrotek |
| author_facet | Tomasz Jędrzejewski Justyna Sobocińska Małgorzata Pawlikowska Artur Dzialuk Sylwia Wrotek |
| author_sort | Tomasz Jędrzejewski |
| collection | DOAJ |
| description | Chronic inflammation is a well-recognised tumour-enabling component, which includes bioactive molecules from cells infiltrating the tumour microenvironment and increases the risk of cancer progression. Since long-term use of the currently available anti-inflammatory drugs used in cancer therapy causes numerous side effects, the aim of this study was to investigate the effect of an extract isolated from the <i>Coriolus versicolor</i> fungus (CV extract) on HUVEC endothelial cells and MCF-7 breast cancer cells in a pro-inflammatory microenvironment mimicked by lipopolysaccharide (LPS). The cells were simultaneously stimulated with the LPS and CV extract. After co-treatment, the cell viability, generation of reactive oxygen species (ROS), wound-healing assay, production of the pro-inflammatory and pro-angiogenic factors (interleukin (IL) 6, IL-8, and metalloproteinase (MMP) 9)), as well as expression of Toll-like receptor (TLR) 4 and phosphorylated IκB (p-IκB) were evaluated. The results showed that the CV extract inhibited IL-6, IL-8, and MMP-9 production by the LPS-stimulated cells. This effect was accompanied by a decrease in TLR4 and p-IκB expression. The CV extract also had anti-migratory properties and induced a cytotoxic effect on the cells that was enhanced in the presence of LPS. The observed cytotoxicity was associated with an increase in ROS generation. We conclude that the CV extract possesses cytotoxic activity against cancer cells and endothelial cells and has the ability to inhibit the expression of the pro-tumorigenic factors associated with inflammation. |
| first_indexed | 2024-03-10T14:28:06Z |
| format | Article |
| id | doaj.art-49c43c14ddb14ca799f5de224d3695a5 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T14:28:06Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-49c43c14ddb14ca799f5de224d3695a52023-11-20T22:47:14ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-11-012123906310.3390/ijms21239063Extract from the <i>Coriolus versicolor</i> Fungus as an Anti-Inflammatory Agent with Cytotoxic Properties against Endothelial Cells and Breast Cancer CellsTomasz Jędrzejewski0Justyna Sobocińska1Małgorzata Pawlikowska2Artur Dzialuk3Sylwia Wrotek4Department of Immunology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University, 1 Lwowska Str., 87-100 Torun, PolandDepartment of Immunology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University, 1 Lwowska Str., 87-100 Torun, PolandDepartment of Immunology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University, 1 Lwowska Str., 87-100 Torun, PolandDepartment of Genetics, Faculty of Biological Sciences, Kazimierz Wielki University, 10 Powstańców Wielkopolskich Ave., 85-090 Bydgoszcz, PolandDepartment of Immunology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University, 1 Lwowska Str., 87-100 Torun, PolandChronic inflammation is a well-recognised tumour-enabling component, which includes bioactive molecules from cells infiltrating the tumour microenvironment and increases the risk of cancer progression. Since long-term use of the currently available anti-inflammatory drugs used in cancer therapy causes numerous side effects, the aim of this study was to investigate the effect of an extract isolated from the <i>Coriolus versicolor</i> fungus (CV extract) on HUVEC endothelial cells and MCF-7 breast cancer cells in a pro-inflammatory microenvironment mimicked by lipopolysaccharide (LPS). The cells were simultaneously stimulated with the LPS and CV extract. After co-treatment, the cell viability, generation of reactive oxygen species (ROS), wound-healing assay, production of the pro-inflammatory and pro-angiogenic factors (interleukin (IL) 6, IL-8, and metalloproteinase (MMP) 9)), as well as expression of Toll-like receptor (TLR) 4 and phosphorylated IκB (p-IκB) were evaluated. The results showed that the CV extract inhibited IL-6, IL-8, and MMP-9 production by the LPS-stimulated cells. This effect was accompanied by a decrease in TLR4 and p-IκB expression. The CV extract also had anti-migratory properties and induced a cytotoxic effect on the cells that was enhanced in the presence of LPS. The observed cytotoxicity was associated with an increase in ROS generation. We conclude that the CV extract possesses cytotoxic activity against cancer cells and endothelial cells and has the ability to inhibit the expression of the pro-tumorigenic factors associated with inflammation.https://www.mdpi.com/1422-0067/21/23/9063<i>Coriolus versicolor</i> extractinflammationlipopolysaccharidebreast cancer cellsendothelial cellsToll-like receptor 4 |
| spellingShingle | Tomasz Jędrzejewski Justyna Sobocińska Małgorzata Pawlikowska Artur Dzialuk Sylwia Wrotek Extract from the <i>Coriolus versicolor</i> Fungus as an Anti-Inflammatory Agent with Cytotoxic Properties against Endothelial Cells and Breast Cancer Cells International Journal of Molecular Sciences <i>Coriolus versicolor</i> extract inflammation lipopolysaccharide breast cancer cells endothelial cells Toll-like receptor 4 |
| title | Extract from the <i>Coriolus versicolor</i> Fungus as an Anti-Inflammatory Agent with Cytotoxic Properties against Endothelial Cells and Breast Cancer Cells |
| title_full | Extract from the <i>Coriolus versicolor</i> Fungus as an Anti-Inflammatory Agent with Cytotoxic Properties against Endothelial Cells and Breast Cancer Cells |
| title_fullStr | Extract from the <i>Coriolus versicolor</i> Fungus as an Anti-Inflammatory Agent with Cytotoxic Properties against Endothelial Cells and Breast Cancer Cells |
| title_full_unstemmed | Extract from the <i>Coriolus versicolor</i> Fungus as an Anti-Inflammatory Agent with Cytotoxic Properties against Endothelial Cells and Breast Cancer Cells |
| title_short | Extract from the <i>Coriolus versicolor</i> Fungus as an Anti-Inflammatory Agent with Cytotoxic Properties against Endothelial Cells and Breast Cancer Cells |
| title_sort | extract from the i coriolus versicolor i fungus as an anti inflammatory agent with cytotoxic properties against endothelial cells and breast cancer cells |
| topic | <i>Coriolus versicolor</i> extract inflammation lipopolysaccharide breast cancer cells endothelial cells Toll-like receptor 4 |
| url | https://www.mdpi.com/1422-0067/21/23/9063 |
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