Investigating applied drought in Miscanthus sinensis; sensitivity, response mechanisms, and subsequent recovery
Abstract Miscanthus is renowned for its excellent water‐use efficiency and good adaptability to a wide range of environmental conditions, making it suitable for cultivation on marginal soils. Drought is a major cause of this marginality, and its occurrence is becoming more frequent and prolonged due...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-07-01
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| Series: | GCB Bioenergy |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/gcbb.12941 |
| _version_ | 1818545955227566080 |
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| author | Mohamad Al Hassan Kasper van derCruijsen Dianka Dees Oene Dolstra Luisa M. Trindade |
| author_facet | Mohamad Al Hassan Kasper van derCruijsen Dianka Dees Oene Dolstra Luisa M. Trindade |
| author_sort | Mohamad Al Hassan |
| collection | DOAJ |
| description | Abstract Miscanthus is renowned for its excellent water‐use efficiency and good adaptability to a wide range of environmental conditions, making it suitable for cultivation on marginal soils. Drought is a major cause of this marginality, and its occurrence is becoming more frequent and prolonged due to climatic change. Developing drought tolerant genotypes of miscanthus would ensure the maintenance of economically viable yields on lands prone to periodic water‐deficiency. To better understand the underlying response and tolerance mechanisms, pre‐screen for better survivability at plot setup on marginal lands, and identifying early biomarkers of stress, we explored the genetic diversity present in Miscanthus sinensis under applied drought. Young plants of 23 genotypes underwent 3 weeks of water‐deprivation in glasshouse‐controlled conditions, followed by an equal period of recovery. Leaves harvested at the end of both experimental phases were the focus of extensive biochemical analyses. Coupled with monitoring several growth and yield parameters, this was instrumental in evaluating stress impact and responses. The most productive genotypes suffered the most in terms of yield reduction and chlorophyll degradation when stress was applied. In parallel, proline and simple soluble sugars accumulated to readjust the osmotic potential in the cytosol and vacuoles, respectively. The necessary carbon skeletons for this buildup were partially acquired from resources diverted away from cell wall synthesis and maintenance, whose content dropped under stress in parallel to increasing drought‐sensitivity. Correspondingly, expressional and biochemical analyses revealed a dynamic turnover of starch and soluble sugars in stressed leaves. Meanwhile, better avoidance of stress enabled a more efficient post‐drought recovery, which was characterized by restoring pre‐stress hydraulic status and unplugging stress response mechanisms. |
| first_indexed | 2024-12-12T07:46:44Z |
| format | Article |
| id | doaj.art-49c9103243eb43e498fc68bfbd20eee4 |
| institution | Directory Open Access Journal |
| issn | 1757-1693 1757-1707 |
| language | English |
| last_indexed | 2024-12-12T07:46:44Z |
| publishDate | 2022-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | GCB Bioenergy |
| spelling | doaj.art-49c9103243eb43e498fc68bfbd20eee42022-12-22T00:32:34ZengWileyGCB Bioenergy1757-16931757-17072022-07-0114775677510.1111/gcbb.12941Investigating applied drought in Miscanthus sinensis; sensitivity, response mechanisms, and subsequent recoveryMohamad Al Hassan0Kasper van derCruijsen1Dianka Dees2Oene Dolstra3Luisa M. Trindade4Laboratory of Plant Breeding Wageningen University & Research (WUR) Wageningen The NetherlandsLaboratory of Plant Breeding Wageningen University & Research (WUR) Wageningen The NetherlandsLaboratory of Plant Breeding Wageningen University & Research (WUR) Wageningen The NetherlandsLaboratory of Plant Breeding Wageningen University & Research (WUR) Wageningen The NetherlandsLaboratory of Plant Breeding Wageningen University & Research (WUR) Wageningen The NetherlandsAbstract Miscanthus is renowned for its excellent water‐use efficiency and good adaptability to a wide range of environmental conditions, making it suitable for cultivation on marginal soils. Drought is a major cause of this marginality, and its occurrence is becoming more frequent and prolonged due to climatic change. Developing drought tolerant genotypes of miscanthus would ensure the maintenance of economically viable yields on lands prone to periodic water‐deficiency. To better understand the underlying response and tolerance mechanisms, pre‐screen for better survivability at plot setup on marginal lands, and identifying early biomarkers of stress, we explored the genetic diversity present in Miscanthus sinensis under applied drought. Young plants of 23 genotypes underwent 3 weeks of water‐deprivation in glasshouse‐controlled conditions, followed by an equal period of recovery. Leaves harvested at the end of both experimental phases were the focus of extensive biochemical analyses. Coupled with monitoring several growth and yield parameters, this was instrumental in evaluating stress impact and responses. The most productive genotypes suffered the most in terms of yield reduction and chlorophyll degradation when stress was applied. In parallel, proline and simple soluble sugars accumulated to readjust the osmotic potential in the cytosol and vacuoles, respectively. The necessary carbon skeletons for this buildup were partially acquired from resources diverted away from cell wall synthesis and maintenance, whose content dropped under stress in parallel to increasing drought‐sensitivity. Correspondingly, expressional and biochemical analyses revealed a dynamic turnover of starch and soluble sugars in stressed leaves. Meanwhile, better avoidance of stress enabled a more efficient post‐drought recovery, which was characterized by restoring pre‐stress hydraulic status and unplugging stress response mechanisms.https://doi.org/10.1111/gcbb.12941biomarkersdroughtmarginal landMiscanthus sinensisrecoverystress response |
| spellingShingle | Mohamad Al Hassan Kasper van derCruijsen Dianka Dees Oene Dolstra Luisa M. Trindade Investigating applied drought in Miscanthus sinensis; sensitivity, response mechanisms, and subsequent recovery GCB Bioenergy biomarkers drought marginal land Miscanthus sinensis recovery stress response |
| title | Investigating applied drought in Miscanthus sinensis; sensitivity, response mechanisms, and subsequent recovery |
| title_full | Investigating applied drought in Miscanthus sinensis; sensitivity, response mechanisms, and subsequent recovery |
| title_fullStr | Investigating applied drought in Miscanthus sinensis; sensitivity, response mechanisms, and subsequent recovery |
| title_full_unstemmed | Investigating applied drought in Miscanthus sinensis; sensitivity, response mechanisms, and subsequent recovery |
| title_short | Investigating applied drought in Miscanthus sinensis; sensitivity, response mechanisms, and subsequent recovery |
| title_sort | investigating applied drought in miscanthus sinensis sensitivity response mechanisms and subsequent recovery |
| topic | biomarkers drought marginal land Miscanthus sinensis recovery stress response |
| url | https://doi.org/10.1111/gcbb.12941 |
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