Toll-Like Receptor (TLR)-9 rs352140 Polymorphism is an Immunopathology Protective Factor in Parkinson's Disease in the Northern Iranian Population
Background: Neuroinflammation and immunopathology in Parkinson's disease (PD) are believed to be associated with genetic and environmental factors. Objective: We conducted the current study to evaluate the Toll-like receptors (TLR4 and TLR9) genes polymorphism in patients with Parkinson'...
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| Format: | Article |
| Language: | English |
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Shiraz University of Medical Sciences
2020-12-01
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| Series: | Iranian Journal of Immunology |
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| Online Access: | https://iji.sums.ac.ir/article_47232_2726e678e6c2e5aab31c5435b9dc5a9d.pdf |
| _version_ | 1818428649426124800 |
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| author | Niloufar Sadat Miri Payam Saadat Abbas Azadmehr Morteza Oladnabi Abdolreza Daraei |
| author_facet | Niloufar Sadat Miri Payam Saadat Abbas Azadmehr Morteza Oladnabi Abdolreza Daraei |
| author_sort | Niloufar Sadat Miri |
| collection | DOAJ |
| description | Background: Neuroinflammation and immunopathology in Parkinson's disease (PD) are believed to be associated with genetic and environmental factors. Objective: We conducted the current study to evaluate the Toll-like receptors (TLR4 and TLR9) genes polymorphism in patients with Parkinson's disease in northern Iran. Methods: We extracted DNA from peripheral blood samples of 100 sporadic cases of Parkinson's disease and 100 healthy-matched controls with the mean age of 69.98 and 71.94 years, respectively. Subsequently, single-nucleotide polymorphisms (SNPs) of TLR4 and TLR9 were genotyped using restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR). Results were confirmed employing Sanger sequencing. For the analysis of our data, we used SNPStats and SPSS 22 software. Results: Our findings indicated that the allele distribution for rs352140 of TLR9 gene was significantly different in the PD group compared with the healthy controls (p=0.02). Moreover, rs352140 T allele was observed to be correlated with PD reduced risk (TT + TC vs. CC). The dominant rs352140 model was approved as the most acceptable inheritance model for fitting the data (OR 0.41, 95% CI 0.23-0.75, p=0.0031). Additionally, haplotype analysis revealed a significant correlation between TLR9 polymorphisms and Parkinson's disease. Conclusion: The results of this study indicated that rs352140T of TLR9 gene was a protective factor in Parkinson's disease. Furthermore, this SNP could be regarded as a prognostic factor. However, this conclusion should be confirmed by further investigations. |
| first_indexed | 2024-12-14T15:04:59Z |
| format | Article |
| id | doaj.art-49cb307423a44fe6926c8a96d6da5219 |
| institution | Directory Open Access Journal |
| issn | 1735-1383 1735-367X |
| language | English |
| last_indexed | 2024-12-14T15:04:59Z |
| publishDate | 2020-12-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Immunology |
| spelling | doaj.art-49cb307423a44fe6926c8a96d6da52192022-12-21T22:56:43ZengShiraz University of Medical SciencesIranian Journal of Immunology1735-13831735-367X2020-12-0117431332310.22034/iji.2020.87454.180347232Toll-Like Receptor (TLR)-9 rs352140 Polymorphism is an Immunopathology Protective Factor in Parkinson's Disease in the Northern Iranian PopulationNiloufar Sadat Miri0Payam Saadat1Abbas Azadmehr2Morteza Oladnabi3Abdolreza Daraei4Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, IranMobility Impairment Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, IranGorgan Congenital Malformations Research Center, Golestan University of Medical Sciences, Gorgan, IranCellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, IranBackground: Neuroinflammation and immunopathology in Parkinson's disease (PD) are believed to be associated with genetic and environmental factors. Objective: We conducted the current study to evaluate the Toll-like receptors (TLR4 and TLR9) genes polymorphism in patients with Parkinson's disease in northern Iran. Methods: We extracted DNA from peripheral blood samples of 100 sporadic cases of Parkinson's disease and 100 healthy-matched controls with the mean age of 69.98 and 71.94 years, respectively. Subsequently, single-nucleotide polymorphisms (SNPs) of TLR4 and TLR9 were genotyped using restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR). Results were confirmed employing Sanger sequencing. For the analysis of our data, we used SNPStats and SPSS 22 software. Results: Our findings indicated that the allele distribution for rs352140 of TLR9 gene was significantly different in the PD group compared with the healthy controls (p=0.02). Moreover, rs352140 T allele was observed to be correlated with PD reduced risk (TT + TC vs. CC). The dominant rs352140 model was approved as the most acceptable inheritance model for fitting the data (OR 0.41, 95% CI 0.23-0.75, p=0.0031). Additionally, haplotype analysis revealed a significant correlation between TLR9 polymorphisms and Parkinson's disease. Conclusion: The results of this study indicated that rs352140T of TLR9 gene was a protective factor in Parkinson's disease. Furthermore, this SNP could be regarded as a prognostic factor. However, this conclusion should be confirmed by further investigations.https://iji.sums.ac.ir/article_47232_2726e678e6c2e5aab31c5435b9dc5a9d.pdfparkinson’s diseasesingle-nucleotide polymorphisms (snps)toll-like receptors 4toll-like receptors 9 |
| spellingShingle | Niloufar Sadat Miri Payam Saadat Abbas Azadmehr Morteza Oladnabi Abdolreza Daraei Toll-Like Receptor (TLR)-9 rs352140 Polymorphism is an Immunopathology Protective Factor in Parkinson's Disease in the Northern Iranian Population Iranian Journal of Immunology parkinson’s disease single-nucleotide polymorphisms (snps) toll-like receptors 4 toll-like receptors 9 |
| title | Toll-Like Receptor (TLR)-9 rs352140 Polymorphism is an Immunopathology Protective Factor in Parkinson's Disease in the Northern Iranian Population |
| title_full | Toll-Like Receptor (TLR)-9 rs352140 Polymorphism is an Immunopathology Protective Factor in Parkinson's Disease in the Northern Iranian Population |
| title_fullStr | Toll-Like Receptor (TLR)-9 rs352140 Polymorphism is an Immunopathology Protective Factor in Parkinson's Disease in the Northern Iranian Population |
| title_full_unstemmed | Toll-Like Receptor (TLR)-9 rs352140 Polymorphism is an Immunopathology Protective Factor in Parkinson's Disease in the Northern Iranian Population |
| title_short | Toll-Like Receptor (TLR)-9 rs352140 Polymorphism is an Immunopathology Protective Factor in Parkinson's Disease in the Northern Iranian Population |
| title_sort | toll like receptor tlr 9 rs352140 polymorphism is an immunopathology protective factor in parkinson s disease in the northern iranian population |
| topic | parkinson’s disease single-nucleotide polymorphisms (snps) toll-like receptors 4 toll-like receptors 9 |
| url | https://iji.sums.ac.ir/article_47232_2726e678e6c2e5aab31c5435b9dc5a9d.pdf |
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