Effect of thymosin alpha-1 on subpopulations of Th1, Th2, Th17, and regulatory T cells (Tregs) in vitro
Thymosin alpha 1 (Tα1) has been shown to have beneficial effects on numerous immune system parameters, but little is known about the effects of Tα1 on patients with gastric carcinoma. The objective of this study was to determine the effect of Tα1 on subpopulations of Th1, Th2, Th17, and regulatory T...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2012-01-01
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000100005&lng=en&tlng=en |
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| author | Xia Yang Feng Qian Hai-Yang He Kai-Jun Liu Yuan-Zhi Lan Bing Ni Yi Tian Xiao-Lan Fu Ji Zhang Zi-Gang Shen Jian Li Yi Yin Jin-Tao Li Yu-Zhang Wu |
| author_facet | Xia Yang Feng Qian Hai-Yang He Kai-Jun Liu Yuan-Zhi Lan Bing Ni Yi Tian Xiao-Lan Fu Ji Zhang Zi-Gang Shen Jian Li Yi Yin Jin-Tao Li Yu-Zhang Wu |
| author_sort | Xia Yang |
| collection | DOAJ |
| description | Thymosin alpha 1 (Tα1) has been shown to have beneficial effects on numerous immune system parameters, but little is known about the effects of Tα1 on patients with gastric carcinoma. The objective of this study was to determine the effect of Tα1 on subpopulations of Th1, Th2, Th17, and regulatory T cells (Tregs) in vitro, and to evaluate its efficacy as an immunoregulatory factor in patients with gastric carcinoma. We compared the effect of Tα1 on the frequency of CD4+ and CD8+ T cells, especially the CD4+CD25+Foxp3+ Tregs in peripheral blood mononuclear cells (PBMCs) from gastric carcinoma patients (N = 35) and healthy donors (N = 22). We also analyzed the changes in the proliferation of PBMCs in response to treatment with Tα1, and examined the production of Th1, Th2, and Th17 cytokines by PBMCs and tumor-infiltrating lymphocytes. The treatment of PBMCs from gastric cancer patients, with Tα1 (50 µg/mL) alone increased the percentage of CD4+CD25+Foxp3+ (suppressive antitumor-specific Tregs) from 1.68 ± 0.697 to 2.19 ± 0.795% (P < 0.05). Our results indicate that Tα1 increases the percentage of Tregs and IL-1β, TNF-α, and IL-6 in vitro. |
| first_indexed | 2024-12-13T13:50:30Z |
| format | Article |
| id | doaj.art-49cb42713ccb4060a80fe9a5290bdb53 |
| institution | Directory Open Access Journal |
| issn | 1414-431X |
| language | English |
| last_indexed | 2024-12-13T13:50:30Z |
| publishDate | 2012-01-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-49cb42713ccb4060a80fe9a5290bdb532022-12-21T23:43:13ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research1414-431X2012-01-01451253210.1590/S0100-879X2012000100005S0100-879X2012000100005Effect of thymosin alpha-1 on subpopulations of Th1, Th2, Th17, and regulatory T cells (Tregs) in vitroXia Yang0Feng Qian1Hai-Yang He2Kai-Jun Liu3Yuan-Zhi Lan4Bing Ni5Yi Tian6Xiao-Lan Fu7Ji Zhang8Zi-Gang Shen9Jian Li10Yi Yin11Jin-Tao Li12Yu-Zhang Wu13Third Military Medical UniversityThird Military Medical UniversityThird Military Medical UniversityThird Military Medical UniversityThird Military Medical UniversityThird Military Medical UniversityThird Military Medical UniversityThird Military Medical UniversityThird Military Medical UniversityThird Military Medical UniversityThird Military Medical UniversityThird Military Medical UniversityThird Military Medical UniversityThird Military Medical UniversityThymosin alpha 1 (Tα1) has been shown to have beneficial effects on numerous immune system parameters, but little is known about the effects of Tα1 on patients with gastric carcinoma. The objective of this study was to determine the effect of Tα1 on subpopulations of Th1, Th2, Th17, and regulatory T cells (Tregs) in vitro, and to evaluate its efficacy as an immunoregulatory factor in patients with gastric carcinoma. We compared the effect of Tα1 on the frequency of CD4+ and CD8+ T cells, especially the CD4+CD25+Foxp3+ Tregs in peripheral blood mononuclear cells (PBMCs) from gastric carcinoma patients (N = 35) and healthy donors (N = 22). We also analyzed the changes in the proliferation of PBMCs in response to treatment with Tα1, and examined the production of Th1, Th2, and Th17 cytokines by PBMCs and tumor-infiltrating lymphocytes. The treatment of PBMCs from gastric cancer patients, with Tα1 (50 µg/mL) alone increased the percentage of CD4+CD25+Foxp3+ (suppressive antitumor-specific Tregs) from 1.68 ± 0.697 to 2.19 ± 0.795% (P < 0.05). Our results indicate that Tα1 increases the percentage of Tregs and IL-1β, TNF-α, and IL-6 in vitro.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000100005&lng=en&tlng=enTα1Gastric carcinomaTregsTh1Th2Th17 |
| spellingShingle | Xia Yang Feng Qian Hai-Yang He Kai-Jun Liu Yuan-Zhi Lan Bing Ni Yi Tian Xiao-Lan Fu Ji Zhang Zi-Gang Shen Jian Li Yi Yin Jin-Tao Li Yu-Zhang Wu Effect of thymosin alpha-1 on subpopulations of Th1, Th2, Th17, and regulatory T cells (Tregs) in vitro Brazilian Journal of Medical and Biological Research Tα1 Gastric carcinoma Tregs Th1 Th2 Th17 |
| title | Effect of thymosin alpha-1 on subpopulations of Th1, Th2, Th17, and regulatory T cells (Tregs) in vitro |
| title_full | Effect of thymosin alpha-1 on subpopulations of Th1, Th2, Th17, and regulatory T cells (Tregs) in vitro |
| title_fullStr | Effect of thymosin alpha-1 on subpopulations of Th1, Th2, Th17, and regulatory T cells (Tregs) in vitro |
| title_full_unstemmed | Effect of thymosin alpha-1 on subpopulations of Th1, Th2, Th17, and regulatory T cells (Tregs) in vitro |
| title_short | Effect of thymosin alpha-1 on subpopulations of Th1, Th2, Th17, and regulatory T cells (Tregs) in vitro |
| title_sort | effect of thymosin alpha 1 on subpopulations of th1 th2 th17 and regulatory t cells tregs in vitro |
| topic | Tα1 Gastric carcinoma Tregs Th1 Th2 Th17 |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000100005&lng=en&tlng=en |
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