Antioxidant and DNA-Protective Activity of an Extract Originated from Kalamon Olives Debittering
Table olives are a major component of the Mediterranean diet and are associated with many beneficial biological activities, which are mainly related to their phenolic compounds. Olive fruit debittering process defines the quantitative and qualitative composition of table olives in biophenols. The ai...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/2/333 |
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| author | Maria Kourti Maria V. Alvanou Zoi Skaperda Fotis Tekos Georgios Papaefstathiou Panagiotis Stathopoulos Demetrios Kouretas |
| author_facet | Maria Kourti Maria V. Alvanou Zoi Skaperda Fotis Tekos Georgios Papaefstathiou Panagiotis Stathopoulos Demetrios Kouretas |
| author_sort | Maria Kourti |
| collection | DOAJ |
| description | Table olives are a major component of the Mediterranean diet and are associated with many beneficial biological activities, which are mainly related to their phenolic compounds. Olive fruit debittering process defines the quantitative and qualitative composition of table olives in biophenols. The aim of the present study was to evaluate the in vitro antioxidant capacity and DNA-protective activity of an extract originated from brine samples, according to the Greek style debbitering process of Kalamon olive fruits. The main phenolic components determined in the brine extract were hydroxytyrosol (HT), verbascoside (VERB) and tyrosol (T). The in vitro cell-free assays showed strong radical scavenging capacity from the extract, therefore antioxidant potential. At cellular level, human endothelial cells (EA.hy296) and murine myoblasts (C2C12) were treated with non-cytotoxic concentrations of the brine extract and the redox status was assessed by measuring glutathione (GSH), reactive oxygen species (ROS) and lipid peroxidation levels (TBARS). Our results show cell type specific response, exerting a hormetic reflection at endothelial cells. Finally, in both cell lines, pre-treatment with brine extract protected from H<sub>2</sub>O<sub>2</sub>-induced DNA damage. In conclusion, this is the first holistic approach highlighted table olive wastewaters from Kalamon- Greek style debittering process, as valuable source of bioactive compounds, which could have interesting implications for the development of new products in food or other industries. |
| first_indexed | 2024-03-11T09:13:54Z |
| format | Article |
| id | doaj.art-49cd7c35a71f4596b4270084a591692d |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-11T09:13:54Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-49cd7c35a71f4596b4270084a591692d2023-11-16T18:46:42ZengMDPI AGAntioxidants2076-39212023-01-0112233310.3390/antiox12020333Antioxidant and DNA-Protective Activity of an Extract Originated from Kalamon Olives DebitteringMaria Kourti0Maria V. Alvanou1Zoi Skaperda2Fotis Tekos3Georgios Papaefstathiou4Panagiotis Stathopoulos5Demetrios Kouretas6Laboratory of Animal Physiology, Department of Biochemistry-Biotechnology, School of Health Sciences, University of Thessaly, 41500 Larissa, GreeceLaboratory of Animal Physiology, Department of Biochemistry-Biotechnology, School of Health Sciences, University of Thessaly, 41500 Larissa, GreeceLaboratory of Animal Physiology, Department of Biochemistry-Biotechnology, School of Health Sciences, University of Thessaly, 41500 Larissa, GreeceLaboratory of Animal Physiology, Department of Biochemistry-Biotechnology, School of Health Sciences, University of Thessaly, 41500 Larissa, GreecePharmaGnose S.A., Papathanasiou 24, 34100 Chalkida, GreeceDepartment of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, University of Athens, 15771 Athens, GreeceLaboratory of Animal Physiology, Department of Biochemistry-Biotechnology, School of Health Sciences, University of Thessaly, 41500 Larissa, GreeceTable olives are a major component of the Mediterranean diet and are associated with many beneficial biological activities, which are mainly related to their phenolic compounds. Olive fruit debittering process defines the quantitative and qualitative composition of table olives in biophenols. The aim of the present study was to evaluate the in vitro antioxidant capacity and DNA-protective activity of an extract originated from brine samples, according to the Greek style debbitering process of Kalamon olive fruits. The main phenolic components determined in the brine extract were hydroxytyrosol (HT), verbascoside (VERB) and tyrosol (T). The in vitro cell-free assays showed strong radical scavenging capacity from the extract, therefore antioxidant potential. At cellular level, human endothelial cells (EA.hy296) and murine myoblasts (C2C12) were treated with non-cytotoxic concentrations of the brine extract and the redox status was assessed by measuring glutathione (GSH), reactive oxygen species (ROS) and lipid peroxidation levels (TBARS). Our results show cell type specific response, exerting a hormetic reflection at endothelial cells. Finally, in both cell lines, pre-treatment with brine extract protected from H<sub>2</sub>O<sub>2</sub>-induced DNA damage. In conclusion, this is the first holistic approach highlighted table olive wastewaters from Kalamon- Greek style debittering process, as valuable source of bioactive compounds, which could have interesting implications for the development of new products in food or other industries.https://www.mdpi.com/2076-3921/12/2/333brine extractKalamon olivesbiophenolsantioxidant activityDNA-protection |
| spellingShingle | Maria Kourti Maria V. Alvanou Zoi Skaperda Fotis Tekos Georgios Papaefstathiou Panagiotis Stathopoulos Demetrios Kouretas Antioxidant and DNA-Protective Activity of an Extract Originated from Kalamon Olives Debittering Antioxidants brine extract Kalamon olives biophenols antioxidant activity DNA-protection |
| title | Antioxidant and DNA-Protective Activity of an Extract Originated from Kalamon Olives Debittering |
| title_full | Antioxidant and DNA-Protective Activity of an Extract Originated from Kalamon Olives Debittering |
| title_fullStr | Antioxidant and DNA-Protective Activity of an Extract Originated from Kalamon Olives Debittering |
| title_full_unstemmed | Antioxidant and DNA-Protective Activity of an Extract Originated from Kalamon Olives Debittering |
| title_short | Antioxidant and DNA-Protective Activity of an Extract Originated from Kalamon Olives Debittering |
| title_sort | antioxidant and dna protective activity of an extract originated from kalamon olives debittering |
| topic | brine extract Kalamon olives biophenols antioxidant activity DNA-protection |
| url | https://www.mdpi.com/2076-3921/12/2/333 |
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