Nanoscale Wetting of Single Viruses
The epidemic spread of many viral infections is mediated by the environmental conditions and influenced by the ambient humidity. Single virus particles have been mainly visualized by atomic force microscopy (AFM) in liquid conditions, where the effect of the relative humidity on virus topography and...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-08-01
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| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/26/17/5184 |
| _version_ | 1797521017950699520 |
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| author | Annalisa Calò Aitziber Eleta-Lopez Thierry Ondarçuhu Albert Verdaguer Alexander M. Bittner |
| author_facet | Annalisa Calò Aitziber Eleta-Lopez Thierry Ondarçuhu Albert Verdaguer Alexander M. Bittner |
| author_sort | Annalisa Calò |
| collection | DOAJ |
| description | The epidemic spread of many viral infections is mediated by the environmental conditions and influenced by the ambient humidity. Single virus particles have been mainly visualized by atomic force microscopy (AFM) in liquid conditions, where the effect of the relative humidity on virus topography and surface cannot be systematically assessed. In this work, we employed multi-frequency AFM, simultaneously with standard topography imaging, to study the nanoscale wetting of individual Tobacco Mosaic virions (TMV) from ambient relative humidity to water condensation (RH > 100%). We recorded amplitude and phase vs. distance curves (APD curves) on top of single virions at various RH and converted them into force vs. distance curves. The high sensitivity of multifrequency AFM to visualize condensed water and sub-micrometer droplets, filling gaps between individual TMV particles at RH > 100%, is demonstrated. Dynamic force spectroscopy allows detecting a thin water layer of thickness ~1 nm, adsorbed on the outer surface of single TMV particles at RH < 60%. |
| first_indexed | 2024-03-10T08:06:44Z |
| format | Article |
| id | doaj.art-49cdab56027044468cd63ccd709da3e8 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T08:06:44Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-49cdab56027044468cd63ccd709da3e82023-11-22T11:00:19ZengMDPI AGMolecules1420-30492021-08-012617518410.3390/molecules26175184Nanoscale Wetting of Single VirusesAnnalisa Calò0Aitziber Eleta-Lopez1Thierry Ondarçuhu2Albert Verdaguer3Alexander M. Bittner4Department of Electronic and Biomedical Engineering, University of Barcelona, Calle Marti i Fraquès 1-11, 08028 Barcelona, SpainCIC nanoGUNE (BRTA), Tolosa Hiribidea 76, 20018 Donostia, SpainInstitut de Mécanique des Fluides de Toulouse (IMFT), Université de Toulouse, CNRS-INPT-UPS, 2 allée du Professeur Camille Soula, 31400 Toulouse, FranceCatalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC and the Barcelona Institute of Science and Technology, Campus UAB, 08193 Bellaterra, SpainCIC nanoGUNE (BRTA), Tolosa Hiribidea 76, 20018 Donostia, SpainThe epidemic spread of many viral infections is mediated by the environmental conditions and influenced by the ambient humidity. Single virus particles have been mainly visualized by atomic force microscopy (AFM) in liquid conditions, where the effect of the relative humidity on virus topography and surface cannot be systematically assessed. In this work, we employed multi-frequency AFM, simultaneously with standard topography imaging, to study the nanoscale wetting of individual Tobacco Mosaic virions (TMV) from ambient relative humidity to water condensation (RH > 100%). We recorded amplitude and phase vs. distance curves (APD curves) on top of single virions at various RH and converted them into force vs. distance curves. The high sensitivity of multifrequency AFM to visualize condensed water and sub-micrometer droplets, filling gaps between individual TMV particles at RH > 100%, is demonstrated. Dynamic force spectroscopy allows detecting a thin water layer of thickness ~1 nm, adsorbed on the outer surface of single TMV particles at RH < 60%.https://www.mdpi.com/1420-3049/26/17/5184Tobacco Mosaic Virus (TMV)nanoscale wettingmultifrequency AFMforce reconstructionamplitude-modulation AM-AFM |
| spellingShingle | Annalisa Calò Aitziber Eleta-Lopez Thierry Ondarçuhu Albert Verdaguer Alexander M. Bittner Nanoscale Wetting of Single Viruses Molecules Tobacco Mosaic Virus (TMV) nanoscale wetting multifrequency AFM force reconstruction amplitude-modulation AM-AFM |
| title | Nanoscale Wetting of Single Viruses |
| title_full | Nanoscale Wetting of Single Viruses |
| title_fullStr | Nanoscale Wetting of Single Viruses |
| title_full_unstemmed | Nanoscale Wetting of Single Viruses |
| title_short | Nanoscale Wetting of Single Viruses |
| title_sort | nanoscale wetting of single viruses |
| topic | Tobacco Mosaic Virus (TMV) nanoscale wetting multifrequency AFM force reconstruction amplitude-modulation AM-AFM |
| url | https://www.mdpi.com/1420-3049/26/17/5184 |
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