Effects of Nanosecond Pulsed Electric Field on Immune Checkpoint Receptors in Melanoma Cells
Checkpoint molecules such as PD-1, LAG-3, and TIM-3 are currently under extensive investigation for their roles in the attenuation of the immune response in cancer. Various methods have been applied to overcome the challenges in this field. This study investigated the effects of nanosecond pulsed el...
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MDPI AG
2023-09-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/16/10/1362 |
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| author | Natalia Sauer Wojciech Szlasa Anna Szewczyk Vitalij Novickij Jolanta Saczko Dagmara Baczyńska Małgorzata Daczewska Julita Kulbacka |
| author_facet | Natalia Sauer Wojciech Szlasa Anna Szewczyk Vitalij Novickij Jolanta Saczko Dagmara Baczyńska Małgorzata Daczewska Julita Kulbacka |
| author_sort | Natalia Sauer |
| collection | DOAJ |
| description | Checkpoint molecules such as PD-1, LAG-3, and TIM-3 are currently under extensive investigation for their roles in the attenuation of the immune response in cancer. Various methods have been applied to overcome the challenges in this field. This study investigated the effects of nanosecond pulsed electric field (nsPEF) treatment on the expression of immune checkpoint molecules in A375 and C32 melanoma cells. The researchers found that the nsPEF treatment was able to enhance membrane permeabilization and morphological changes in the cell membrane without being cytotoxic. We found that the effects of nsPEFs on melanoma included (1) the transport of vesicles from the inside to the outside of the cells, (2) cell contraction, and (3) the migration of lipids from inside the cells to their peripheries. The treatment increased the expression of PD-1 checkpoint receptors. Furthermore, we also observed potential co-localization or clustering of MHC class II and PD-1 molecules on the cell surface and the secretion of cytokines such as TNF-α and IL-6. These findings suggest that nsPEF treatment could be a viable approach to enhance the delivery of therapeutic agents to cancer cells and to modulate the tumor microenvironment to promote an antitumor immune response. Further studies are needed to explore the mechanisms underlying these effects and their impacts on the antitumor immune response, and to investigate the potential of nsPEF treatment in combination with immune checkpoint inhibitors to improve clinical outcomes for cancer patients. |
| first_indexed | 2024-03-10T20:58:34Z |
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| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-10T20:58:34Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-49cffe11c10a4d96ac3571ec35010b072023-11-19T17:41:24ZengMDPI AGPharmaceuticals1424-82472023-09-011610136210.3390/ph16101362Effects of Nanosecond Pulsed Electric Field on Immune Checkpoint Receptors in Melanoma CellsNatalia Sauer0Wojciech Szlasa1Anna Szewczyk2Vitalij Novickij3Jolanta Saczko4Dagmara Baczyńska5Małgorzata Daczewska6Julita Kulbacka7Faculty of Pharmacy, Wroclaw Medical University, 50-556 Wroclaw, PolandFaculty of Medicine, Wroclaw Medical University, 50-556 Wroclaw, PolandDepartment of Molecular and Cellular Biology, Faculty of Pharmacy, Wroclaw Medical University, 51-618 Wroclaw, PolandInstitute of High Magnetic Fields, Vilnius Gediminas Technical University, 08217 Vilnius, LithuaniaDepartment of Molecular and Cellular Biology, Faculty of Pharmacy, Wroclaw Medical University, 51-618 Wroclaw, PolandDepartment of Molecular and Cellular Biology, Faculty of Pharmacy, Wroclaw Medical University, 51-618 Wroclaw, PolandDepartment of Animal Developmental Biology, Faculty of Biological Sciences, University of Wroclaw, Sienkiewicza 21, 50-335 Wroclaw, PolandDepartment of Molecular and Cellular Biology, Faculty of Pharmacy, Wroclaw Medical University, 51-618 Wroclaw, PolandCheckpoint molecules such as PD-1, LAG-3, and TIM-3 are currently under extensive investigation for their roles in the attenuation of the immune response in cancer. Various methods have been applied to overcome the challenges in this field. This study investigated the effects of nanosecond pulsed electric field (nsPEF) treatment on the expression of immune checkpoint molecules in A375 and C32 melanoma cells. The researchers found that the nsPEF treatment was able to enhance membrane permeabilization and morphological changes in the cell membrane without being cytotoxic. We found that the effects of nsPEFs on melanoma included (1) the transport of vesicles from the inside to the outside of the cells, (2) cell contraction, and (3) the migration of lipids from inside the cells to their peripheries. The treatment increased the expression of PD-1 checkpoint receptors. Furthermore, we also observed potential co-localization or clustering of MHC class II and PD-1 molecules on the cell surface and the secretion of cytokines such as TNF-α and IL-6. These findings suggest that nsPEF treatment could be a viable approach to enhance the delivery of therapeutic agents to cancer cells and to modulate the tumor microenvironment to promote an antitumor immune response. Further studies are needed to explore the mechanisms underlying these effects and their impacts on the antitumor immune response, and to investigate the potential of nsPEF treatment in combination with immune checkpoint inhibitors to improve clinical outcomes for cancer patients.https://www.mdpi.com/1424-8247/16/10/1362pulsed electric fieldmelanomaPD-1LAG-3TIM-3checkpoint receptors |
| spellingShingle | Natalia Sauer Wojciech Szlasa Anna Szewczyk Vitalij Novickij Jolanta Saczko Dagmara Baczyńska Małgorzata Daczewska Julita Kulbacka Effects of Nanosecond Pulsed Electric Field on Immune Checkpoint Receptors in Melanoma Cells Pharmaceuticals pulsed electric field melanoma PD-1 LAG-3 TIM-3 checkpoint receptors |
| title | Effects of Nanosecond Pulsed Electric Field on Immune Checkpoint Receptors in Melanoma Cells |
| title_full | Effects of Nanosecond Pulsed Electric Field on Immune Checkpoint Receptors in Melanoma Cells |
| title_fullStr | Effects of Nanosecond Pulsed Electric Field on Immune Checkpoint Receptors in Melanoma Cells |
| title_full_unstemmed | Effects of Nanosecond Pulsed Electric Field on Immune Checkpoint Receptors in Melanoma Cells |
| title_short | Effects of Nanosecond Pulsed Electric Field on Immune Checkpoint Receptors in Melanoma Cells |
| title_sort | effects of nanosecond pulsed electric field on immune checkpoint receptors in melanoma cells |
| topic | pulsed electric field melanoma PD-1 LAG-3 TIM-3 checkpoint receptors |
| url | https://www.mdpi.com/1424-8247/16/10/1362 |
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