Circular RNA Expression Profiling and Selection of Key Circular RNAs in the Hypothalamus of Heat-Acclimated Rats
Circular RNAs (circRNAs) have vital roles in great variety of biological processes. However, expression levels and functions of circRNAs related to heat acclimation (HA) are poorly understood. This study is the first time an in-depth circRNA expression profiling were used to investigate circRNA–miRN...
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| Format: | Article |
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Frontiers Media S.A.
2019-08-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2019.01112/full |
| _version_ | 1828740615544766464 |
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| author | Lijun Fan Lijun Fan Gaihong An Shang Wang Xuewei Chen Ying Liu Zhifeng Liu Qiang Ma Jing Wang |
| author_facet | Lijun Fan Lijun Fan Gaihong An Shang Wang Xuewei Chen Ying Liu Zhifeng Liu Qiang Ma Jing Wang |
| author_sort | Lijun Fan |
| collection | DOAJ |
| description | Circular RNAs (circRNAs) have vital roles in great variety of biological processes. However, expression levels and functions of circRNAs related to heat acclimation (HA) are poorly understood. This study is the first time an in-depth circRNA expression profiling were used to investigate circRNA–miRNA interactions in HA rats in order to further comprehend the mechanisms underlying HA. CircRNA expression profile was performed in rats’ hypothalamus of HA and control group with microarray assays and their functions were predicted by using Bioinformatics analysis. Differential circRNAs and their regulated downstream miRNAs and mRNAs were quantitatively validated by means of quantitative polymerase chain reaction in real-time (RT-qPCR). Enzyme-linked immunosorbent assay (ELISA) was then applied to predict the expression of proteins. In total, 53 circRNAs were expressed distinctively between the HA and Control; up- and down-regulation of circRNAs were 28 and 25, respectively, in HA (fold change > 1.5, P < 0.05). Three circRNAs and two miRNAs and three predicted mRNAs were obviously regulated after validated by RT-qPCR in HA rats. Two proteins expression were proportional to their mRNA changes. Further analysis demonstrates that circRNAs closest to HA can be categorized into three signal pathways: including rno_circRNA_014301-vs-rno-miR-3575-vs-Hif-1α, rno_circRNA_014301-vs-rno-miR-3575-vs-Lppr4, and rno_circRNA_010393-vs-rno-miR-20b-3p-vs-Mfap4 in hypoxia response pathways, substance/energy metabolism, and inflammatory response pathways. Our findings implicate that many circRNAs regulate expressions of genes that interact with each other to exert their functions during HA. |
| first_indexed | 2024-04-13T00:44:12Z |
| format | Article |
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| issn | 1664-042X |
| language | English |
| last_indexed | 2024-04-13T00:44:12Z |
| publishDate | 2019-08-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Physiology |
| spelling | doaj.art-49d3096caecc40eb90f841ac81861ead2022-12-22T03:10:04ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2019-08-011010.3389/fphys.2019.01112469409Circular RNA Expression Profiling and Selection of Key Circular RNAs in the Hypothalamus of Heat-Acclimated RatsLijun Fan0Lijun Fan1Gaihong An2Shang Wang3Xuewei Chen4Ying Liu5Zhifeng Liu6Qiang Ma7Jing Wang8Department of Operational Medicine, Tianjin Institute of Environmental and Operational Medicine, Tianjin, ChinaDepartment of Human Movement Science, Tianjin University of Sport, Tianjin, ChinaDepartment of Operational Medicine, Tianjin Institute of Environmental and Operational Medicine, Tianjin, ChinaDepartment of Operational Medicine, Tianjin Institute of Environmental and Operational Medicine, Tianjin, ChinaDepartment of Operational Medicine, Tianjin Institute of Environmental and Operational Medicine, Tianjin, ChinaDepartment of Operational Medicine, Tianjin Institute of Environmental and Operational Medicine, Tianjin, ChinaDepartment of Intensive Care Medicine, General Hospital of Southern Theatre Command of People’s Liberation Army, Guangzhou, ChinaDepartment of Operational Medicine, Tianjin Institute of Environmental and Operational Medicine, Tianjin, ChinaDepartment of Operational Medicine, Tianjin Institute of Environmental and Operational Medicine, Tianjin, ChinaCircular RNAs (circRNAs) have vital roles in great variety of biological processes. However, expression levels and functions of circRNAs related to heat acclimation (HA) are poorly understood. This study is the first time an in-depth circRNA expression profiling were used to investigate circRNA–miRNA interactions in HA rats in order to further comprehend the mechanisms underlying HA. CircRNA expression profile was performed in rats’ hypothalamus of HA and control group with microarray assays and their functions were predicted by using Bioinformatics analysis. Differential circRNAs and their regulated downstream miRNAs and mRNAs were quantitatively validated by means of quantitative polymerase chain reaction in real-time (RT-qPCR). Enzyme-linked immunosorbent assay (ELISA) was then applied to predict the expression of proteins. In total, 53 circRNAs were expressed distinctively between the HA and Control; up- and down-regulation of circRNAs were 28 and 25, respectively, in HA (fold change > 1.5, P < 0.05). Three circRNAs and two miRNAs and three predicted mRNAs were obviously regulated after validated by RT-qPCR in HA rats. Two proteins expression were proportional to their mRNA changes. Further analysis demonstrates that circRNAs closest to HA can be categorized into three signal pathways: including rno_circRNA_014301-vs-rno-miR-3575-vs-Hif-1α, rno_circRNA_014301-vs-rno-miR-3575-vs-Lppr4, and rno_circRNA_010393-vs-rno-miR-20b-3p-vs-Mfap4 in hypoxia response pathways, substance/energy metabolism, and inflammatory response pathways. Our findings implicate that many circRNAs regulate expressions of genes that interact with each other to exert their functions during HA.https://www.frontiersin.org/article/10.3389/fphys.2019.01112/fullhigh-throughput RNA sequencing technologycircRNAsheat acclimationmRNAmicroRNA |
| spellingShingle | Lijun Fan Lijun Fan Gaihong An Shang Wang Xuewei Chen Ying Liu Zhifeng Liu Qiang Ma Jing Wang Circular RNA Expression Profiling and Selection of Key Circular RNAs in the Hypothalamus of Heat-Acclimated Rats Frontiers in Physiology high-throughput RNA sequencing technology circRNAs heat acclimation mRNA microRNA |
| title | Circular RNA Expression Profiling and Selection of Key Circular RNAs in the Hypothalamus of Heat-Acclimated Rats |
| title_full | Circular RNA Expression Profiling and Selection of Key Circular RNAs in the Hypothalamus of Heat-Acclimated Rats |
| title_fullStr | Circular RNA Expression Profiling and Selection of Key Circular RNAs in the Hypothalamus of Heat-Acclimated Rats |
| title_full_unstemmed | Circular RNA Expression Profiling and Selection of Key Circular RNAs in the Hypothalamus of Heat-Acclimated Rats |
| title_short | Circular RNA Expression Profiling and Selection of Key Circular RNAs in the Hypothalamus of Heat-Acclimated Rats |
| title_sort | circular rna expression profiling and selection of key circular rnas in the hypothalamus of heat acclimated rats |
| topic | high-throughput RNA sequencing technology circRNAs heat acclimation mRNA microRNA |
| url | https://www.frontiersin.org/article/10.3389/fphys.2019.01112/full |
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