Antagonistic activity of endophytic actinobacteria from native potatoes (Solanum tuberosum subsp. tuberosum L.) against Pectobacterium carotovorum subsp. carotovorum and Pectobacterium atrosepticum
Abstract Background The native potatoes (Solanum tuberosum subsp. tuberosum L.) grown in Chile (Chiloé) represent a new, unexplored source of endophytes to find potential biological control agents for the prevention of bacterial diseases, like blackleg and soft rot, in potato crops. Result The objec...
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| Format: | Article |
| Language: | English |
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BMC
2021-12-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-021-02393-x |
| _version_ | 1818575249244946432 |
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| author | Natalia Padilla-Gálvez Paola Luengo-Uribe Sandra Mancilla Amandine Maurin Claudia Torres Pamela Ruiz Andrés France Ivette Acuña Homero Urrutia |
| author_facet | Natalia Padilla-Gálvez Paola Luengo-Uribe Sandra Mancilla Amandine Maurin Claudia Torres Pamela Ruiz Andrés France Ivette Acuña Homero Urrutia |
| author_sort | Natalia Padilla-Gálvez |
| collection | DOAJ |
| description | Abstract Background The native potatoes (Solanum tuberosum subsp. tuberosum L.) grown in Chile (Chiloé) represent a new, unexplored source of endophytes to find potential biological control agents for the prevention of bacterial diseases, like blackleg and soft rot, in potato crops. Result The objective of this study was the selection of endophytic actinobacteria from native potatoes for antagonistic activity against Pectobacterium carotovorum subsp. carotovorum and Pectobacterium atrosepticum, and their potential to suppress tissue maceration symptoms in potato tubers. This potential was determined through the quorum quenching activity using a Chromobacterium violaceaum ATCC 12472 Wild type (WT) bioassay and its colonization behavior of the potato plant root system (S. tuberosum) by means of the Double labeling of oligonucleotide probes for fluorescence in situ hybridization (DOPE-FISH) targeting technique. The results showed that although Streptomyces sp. TP199 and Streptomyces sp. A2R31 were able to inhibit the growth of the pathogens, only the Streptomyces sp. TP199 isolate inhibited Pectobacterium sp. growth and diminished tissue maceration in tubers (p ≤ 0.05). Streptomyces sp. TP199 had metal-dependent acyl homoserine lactones (AHL) quorum quenching activity in vitro and was able to colonize the root endosphere 10 days after inoculation. Conclusions We concluded that native potatoes from southern Chile possess endophyte actinobacteria that are potential agents for the disease management of soft rot and blackleg. |
| first_indexed | 2024-12-15T00:37:40Z |
| format | Article |
| id | doaj.art-49d3e1b14471492f9b8e7814c04d904a |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-15T00:37:40Z |
| publishDate | 2021-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-49d3e1b14471492f9b8e7814c04d904a2022-12-21T22:41:44ZengBMCBMC Microbiology1471-21802021-12-0121111710.1186/s12866-021-02393-xAntagonistic activity of endophytic actinobacteria from native potatoes (Solanum tuberosum subsp. tuberosum L.) against Pectobacterium carotovorum subsp. carotovorum and Pectobacterium atrosepticumNatalia Padilla-Gálvez0Paola Luengo-Uribe1Sandra Mancilla2Amandine Maurin3Claudia Torres4Pamela Ruiz5Andrés France6Ivette Acuña7Homero Urrutia8Laboratorio de Biopelículas y Microbiología Ambiental, Centro de Biotecnología, Universidad de ConcepciónLaboratorio de Biopelículas y Microbiología Ambiental, Centro de Biotecnología, Universidad de ConcepciónInstituto de Investigaciones AgropecuariasLaboratorio de Biopelículas y Microbiología Ambiental, Centro de Biotecnología, Universidad de ConcepciónLaboratorio de Biopelículas y Microbiología Ambiental, Centro de Biotecnología, Universidad de ConcepciónLaboratorio de Biopelículas y Microbiología Ambiental, Centro de Biotecnología, Universidad de ConcepciónInstituto de Investigaciones Agropecuarias, INIA QuilamapuInstituto de Investigaciones AgropecuariasLaboratorio de Biopelículas y Microbiología Ambiental, Centro de Biotecnología, Universidad de ConcepciónAbstract Background The native potatoes (Solanum tuberosum subsp. tuberosum L.) grown in Chile (Chiloé) represent a new, unexplored source of endophytes to find potential biological control agents for the prevention of bacterial diseases, like blackleg and soft rot, in potato crops. Result The objective of this study was the selection of endophytic actinobacteria from native potatoes for antagonistic activity against Pectobacterium carotovorum subsp. carotovorum and Pectobacterium atrosepticum, and their potential to suppress tissue maceration symptoms in potato tubers. This potential was determined through the quorum quenching activity using a Chromobacterium violaceaum ATCC 12472 Wild type (WT) bioassay and its colonization behavior of the potato plant root system (S. tuberosum) by means of the Double labeling of oligonucleotide probes for fluorescence in situ hybridization (DOPE-FISH) targeting technique. The results showed that although Streptomyces sp. TP199 and Streptomyces sp. A2R31 were able to inhibit the growth of the pathogens, only the Streptomyces sp. TP199 isolate inhibited Pectobacterium sp. growth and diminished tissue maceration in tubers (p ≤ 0.05). Streptomyces sp. TP199 had metal-dependent acyl homoserine lactones (AHL) quorum quenching activity in vitro and was able to colonize the root endosphere 10 days after inoculation. Conclusions We concluded that native potatoes from southern Chile possess endophyte actinobacteria that are potential agents for the disease management of soft rot and blackleg.https://doi.org/10.1186/s12866-021-02393-xEndophytic actinobacteriaStreptomyces sp.Quorum quenchingConfocal laser microscopyBlacklegSoft rot |
| spellingShingle | Natalia Padilla-Gálvez Paola Luengo-Uribe Sandra Mancilla Amandine Maurin Claudia Torres Pamela Ruiz Andrés France Ivette Acuña Homero Urrutia Antagonistic activity of endophytic actinobacteria from native potatoes (Solanum tuberosum subsp. tuberosum L.) against Pectobacterium carotovorum subsp. carotovorum and Pectobacterium atrosepticum BMC Microbiology Endophytic actinobacteria Streptomyces sp. Quorum quenching Confocal laser microscopy Blackleg Soft rot |
| title | Antagonistic activity of endophytic actinobacteria from native potatoes (Solanum tuberosum subsp. tuberosum L.) against Pectobacterium carotovorum subsp. carotovorum and Pectobacterium atrosepticum |
| title_full | Antagonistic activity of endophytic actinobacteria from native potatoes (Solanum tuberosum subsp. tuberosum L.) against Pectobacterium carotovorum subsp. carotovorum and Pectobacterium atrosepticum |
| title_fullStr | Antagonistic activity of endophytic actinobacteria from native potatoes (Solanum tuberosum subsp. tuberosum L.) against Pectobacterium carotovorum subsp. carotovorum and Pectobacterium atrosepticum |
| title_full_unstemmed | Antagonistic activity of endophytic actinobacteria from native potatoes (Solanum tuberosum subsp. tuberosum L.) against Pectobacterium carotovorum subsp. carotovorum and Pectobacterium atrosepticum |
| title_short | Antagonistic activity of endophytic actinobacteria from native potatoes (Solanum tuberosum subsp. tuberosum L.) against Pectobacterium carotovorum subsp. carotovorum and Pectobacterium atrosepticum |
| title_sort | antagonistic activity of endophytic actinobacteria from native potatoes solanum tuberosum subsp tuberosum l against pectobacterium carotovorum subsp carotovorum and pectobacterium atrosepticum |
| topic | Endophytic actinobacteria Streptomyces sp. Quorum quenching Confocal laser microscopy Blackleg Soft rot |
| url | https://doi.org/10.1186/s12866-021-02393-x |
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