Transcriptomic Analysis Reveals Candidate Genes in Response to Sorghum Mosaic Virus and Salicylic Acid in Sugarcane
Sorghum mosaic virus (SrMV) is one of the most prevalent viruses deteriorating sugarcane production. Salicylic acid (SA) plays an essential role in the defense mechanism of plants and its exogenous application has been observed to induce the resistance against biotic and abiotic stressors. In this s...
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| Format: | Article |
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MDPI AG
2024-01-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/13/2/234 |
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| author | Genhua Zhou Rubab Shabbir Zihao Sun Yating Chang Xinli Liu Pinghua Chen |
| author_facet | Genhua Zhou Rubab Shabbir Zihao Sun Yating Chang Xinli Liu Pinghua Chen |
| author_sort | Genhua Zhou |
| collection | DOAJ |
| description | Sorghum mosaic virus (SrMV) is one of the most prevalent viruses deteriorating sugarcane production. Salicylic acid (SA) plays an essential role in the defense mechanism of plants and its exogenous application has been observed to induce the resistance against biotic and abiotic stressors. In this study, we set out to investigate the mechanism by which sorghum mosaic virus (SrMV) infected sugarcane responds to SA treatment in two sugarcane cultivars, i.e., ROC22 and Xuezhe. Notably, significantly low viral populations were observed at different time points (except for 28 d in ROC22) in response to post-SA application in both cultivars as compared to control based on qPCR data. Furthermore, the lowest number of population size in Xuezhe (20 copies/µL) and ROC22 (95 copies/µL) was observed in response to 1 mM exogenous SA application. A total of 2999 DEGs were identified, of which 731 and 2268 DEGs were up- and down-regulated, respectively. Moreover, a total of 806 DEGs were annotated to GO enrichment categories: 348 biological processes, 280 molecular functions, and 178 cellular components. GO functional categorization revealed that DEGs were mainly enriched in metabolic processes, extracellular regions, and glucosyltransferase activity, while KEGG annotation revealed that DEGs were mainly concentrated in phenylpropanoid biosynthesis and plant-pathogen interaction suggesting the involvement of these pathways in SA-induced disease resistance of sugarcane in response to SrMV infection. The RNA-seq dataset and qRT-PCR assay showed that the transcript levels of <i>PR1a</i>, <i>PR1b</i>, <i>PR1c</i>, <i>NPR1a</i>, <i>NPR1b</i>, <i>PAL</i>, <i>ICS,</i> and <i>ABA</i> were significantly up-regulated in response to SA treatment under SrMV infection, indicating their positive involvement in stress endorsement. Overall, this research characterized sugarcane transcriptome during SrMV infection and shed light on further interaction of plant-pathogen under exogenous application of SA treatment. |
| first_indexed | 2024-03-08T09:47:41Z |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-08T09:47:41Z |
| publishDate | 2024-01-01 |
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| spelling | doaj.art-49d4a50dc69b44898ded71f9ead2a6e12024-01-29T14:11:13ZengMDPI AGPlants2223-77472024-01-0113223410.3390/plants13020234Transcriptomic Analysis Reveals Candidate Genes in Response to Sorghum Mosaic Virus and Salicylic Acid in SugarcaneGenhua Zhou0Rubab Shabbir1Zihao Sun2Yating Chang3Xinli Liu4Pinghua Chen5Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, National Engineering Research Center for Sugarcane, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, National Engineering Research Center for Sugarcane, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, National Engineering Research Center for Sugarcane, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, National Engineering Research Center for Sugarcane, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, National Engineering Research Center for Sugarcane, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, National Engineering Research Center for Sugarcane, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaSorghum mosaic virus (SrMV) is one of the most prevalent viruses deteriorating sugarcane production. Salicylic acid (SA) plays an essential role in the defense mechanism of plants and its exogenous application has been observed to induce the resistance against biotic and abiotic stressors. In this study, we set out to investigate the mechanism by which sorghum mosaic virus (SrMV) infected sugarcane responds to SA treatment in two sugarcane cultivars, i.e., ROC22 and Xuezhe. Notably, significantly low viral populations were observed at different time points (except for 28 d in ROC22) in response to post-SA application in both cultivars as compared to control based on qPCR data. Furthermore, the lowest number of population size in Xuezhe (20 copies/µL) and ROC22 (95 copies/µL) was observed in response to 1 mM exogenous SA application. A total of 2999 DEGs were identified, of which 731 and 2268 DEGs were up- and down-regulated, respectively. Moreover, a total of 806 DEGs were annotated to GO enrichment categories: 348 biological processes, 280 molecular functions, and 178 cellular components. GO functional categorization revealed that DEGs were mainly enriched in metabolic processes, extracellular regions, and glucosyltransferase activity, while KEGG annotation revealed that DEGs were mainly concentrated in phenylpropanoid biosynthesis and plant-pathogen interaction suggesting the involvement of these pathways in SA-induced disease resistance of sugarcane in response to SrMV infection. The RNA-seq dataset and qRT-PCR assay showed that the transcript levels of <i>PR1a</i>, <i>PR1b</i>, <i>PR1c</i>, <i>NPR1a</i>, <i>NPR1b</i>, <i>PAL</i>, <i>ICS,</i> and <i>ABA</i> were significantly up-regulated in response to SA treatment under SrMV infection, indicating their positive involvement in stress endorsement. Overall, this research characterized sugarcane transcriptome during SrMV infection and shed light on further interaction of plant-pathogen under exogenous application of SA treatment.https://www.mdpi.com/2223-7747/13/2/234sugarcanesorghum mosaic virussalicylic acidtranscriptomics |
| spellingShingle | Genhua Zhou Rubab Shabbir Zihao Sun Yating Chang Xinli Liu Pinghua Chen Transcriptomic Analysis Reveals Candidate Genes in Response to Sorghum Mosaic Virus and Salicylic Acid in Sugarcane Plants sugarcane sorghum mosaic virus salicylic acid transcriptomics |
| title | Transcriptomic Analysis Reveals Candidate Genes in Response to Sorghum Mosaic Virus and Salicylic Acid in Sugarcane |
| title_full | Transcriptomic Analysis Reveals Candidate Genes in Response to Sorghum Mosaic Virus and Salicylic Acid in Sugarcane |
| title_fullStr | Transcriptomic Analysis Reveals Candidate Genes in Response to Sorghum Mosaic Virus and Salicylic Acid in Sugarcane |
| title_full_unstemmed | Transcriptomic Analysis Reveals Candidate Genes in Response to Sorghum Mosaic Virus and Salicylic Acid in Sugarcane |
| title_short | Transcriptomic Analysis Reveals Candidate Genes in Response to Sorghum Mosaic Virus and Salicylic Acid in Sugarcane |
| title_sort | transcriptomic analysis reveals candidate genes in response to sorghum mosaic virus and salicylic acid in sugarcane |
| topic | sugarcane sorghum mosaic virus salicylic acid transcriptomics |
| url | https://www.mdpi.com/2223-7747/13/2/234 |
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