Glucose Biosensor Based on Disposable Activated Carbon Electrodes Modified with Platinum Nanoparticles Electrodeposited on Poly(Azure A)
Herein, a novel electrochemical glucose biosensor based on glucose oxidase (GOx) immobilized on a surface containing platinum nanoparticles (PtNPs) electrodeposited on poly(Azure A) (PAA) previously electropolymerized on activated screen-printed carbon electrodes (GOx-PtNPs-PAA-aSPCEs) is reported....
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-08-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/20/16/4489 |
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| author | Francisco Jiménez-Fiérrez María Isabel González-Sánchez Rebeca Jiménez-Pérez Jesús Iniesta Edelmira Valero |
| author_facet | Francisco Jiménez-Fiérrez María Isabel González-Sánchez Rebeca Jiménez-Pérez Jesús Iniesta Edelmira Valero |
| author_sort | Francisco Jiménez-Fiérrez |
| collection | DOAJ |
| description | Herein, a novel electrochemical glucose biosensor based on glucose oxidase (GOx) immobilized on a surface containing platinum nanoparticles (PtNPs) electrodeposited on poly(Azure A) (PAA) previously electropolymerized on activated screen-printed carbon electrodes (GOx-PtNPs-PAA-aSPCEs) is reported. The resulting electrochemical biosensor was validated towards glucose oxidation in real samples and further electrochemical measurement associated with the generated H<sub>2</sub>O<sub>2</sub>. The electrochemical biosensor showed an excellent sensitivity (42.7 μA mM<sup>−1</sup> cm<sup>−2</sup>), limit of detection (7.6 μM), linear range (20 μM–2.3 mM), and good selectivity towards glucose determination. Furthermore, and most importantly, the detection of glucose was performed at a low potential (0.2 V vs. Ag). The high performance of the electrochemical biosensor was explained through surface exploration using field emission SEM, XPS, and impedance measurements. The electrochemical biosensor was successfully applied to glucose quantification in several real samples (commercial juices and a plant cell culture medium), exhibiting a high accuracy when compared with a classical spectrophotometric method. This electrochemical biosensor can be easily prepared and opens up a good alternative in the development of new sensitive glucose sensors. |
| first_indexed | 2024-03-10T17:37:23Z |
| format | Article |
| id | doaj.art-49d74930212e4fa3aa25fed595258f63 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-10T17:37:23Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-49d74930212e4fa3aa25fed595258f632023-11-20T09:47:21ZengMDPI AGSensors1424-82202020-08-012016448910.3390/s20164489Glucose Biosensor Based on Disposable Activated Carbon Electrodes Modified with Platinum Nanoparticles Electrodeposited on Poly(Azure A)Francisco Jiménez-Fiérrez0María Isabel González-Sánchez1Rebeca Jiménez-Pérez2Jesús Iniesta3Edelmira Valero4Department of Physical Chemistry, Higher Technical School of Industrial Engineering, University of Castilla-La Mancha, Campus Universitario s/n, 02071 Albacete, SpainDepartment of Physical Chemistry, Higher Technical School of Industrial Engineering, University of Castilla-La Mancha, Campus Universitario s/n, 02071 Albacete, SpainDepartment of Physical Chemistry, Higher Technical School of Industrial Engineering, University of Castilla-La Mancha, Campus Universitario s/n, 02071 Albacete, SpainDepartment of Physical Chemistry and Institute of Electrochemistry, University of Alicante, 03690 San Vicente del Raspeig, SpainDepartment of Physical Chemistry, Higher Technical School of Industrial Engineering, University of Castilla-La Mancha, Campus Universitario s/n, 02071 Albacete, SpainHerein, a novel electrochemical glucose biosensor based on glucose oxidase (GOx) immobilized on a surface containing platinum nanoparticles (PtNPs) electrodeposited on poly(Azure A) (PAA) previously electropolymerized on activated screen-printed carbon electrodes (GOx-PtNPs-PAA-aSPCEs) is reported. The resulting electrochemical biosensor was validated towards glucose oxidation in real samples and further electrochemical measurement associated with the generated H<sub>2</sub>O<sub>2</sub>. The electrochemical biosensor showed an excellent sensitivity (42.7 μA mM<sup>−1</sup> cm<sup>−2</sup>), limit of detection (7.6 μM), linear range (20 μM–2.3 mM), and good selectivity towards glucose determination. Furthermore, and most importantly, the detection of glucose was performed at a low potential (0.2 V vs. Ag). The high performance of the electrochemical biosensor was explained through surface exploration using field emission SEM, XPS, and impedance measurements. The electrochemical biosensor was successfully applied to glucose quantification in several real samples (commercial juices and a plant cell culture medium), exhibiting a high accuracy when compared with a classical spectrophotometric method. This electrochemical biosensor can be easily prepared and opens up a good alternative in the development of new sensitive glucose sensors.https://www.mdpi.com/1424-8220/20/16/4489glucoseglucose oxidaseenzymatic biosensorpoly(Azure A)platinum nanoparticlesactivated screen-printed carbon electrodes |
| spellingShingle | Francisco Jiménez-Fiérrez María Isabel González-Sánchez Rebeca Jiménez-Pérez Jesús Iniesta Edelmira Valero Glucose Biosensor Based on Disposable Activated Carbon Electrodes Modified with Platinum Nanoparticles Electrodeposited on Poly(Azure A) Sensors glucose glucose oxidase enzymatic biosensor poly(Azure A) platinum nanoparticles activated screen-printed carbon electrodes |
| title | Glucose Biosensor Based on Disposable Activated Carbon Electrodes Modified with Platinum Nanoparticles Electrodeposited on Poly(Azure A) |
| title_full | Glucose Biosensor Based on Disposable Activated Carbon Electrodes Modified with Platinum Nanoparticles Electrodeposited on Poly(Azure A) |
| title_fullStr | Glucose Biosensor Based on Disposable Activated Carbon Electrodes Modified with Platinum Nanoparticles Electrodeposited on Poly(Azure A) |
| title_full_unstemmed | Glucose Biosensor Based on Disposable Activated Carbon Electrodes Modified with Platinum Nanoparticles Electrodeposited on Poly(Azure A) |
| title_short | Glucose Biosensor Based on Disposable Activated Carbon Electrodes Modified with Platinum Nanoparticles Electrodeposited on Poly(Azure A) |
| title_sort | glucose biosensor based on disposable activated carbon electrodes modified with platinum nanoparticles electrodeposited on poly azure a |
| topic | glucose glucose oxidase enzymatic biosensor poly(Azure A) platinum nanoparticles activated screen-printed carbon electrodes |
| url | https://www.mdpi.com/1424-8220/20/16/4489 |
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