A 33-residue peptide tag increases solubility and stability of Escherichia coli produced single-chain antibody fragments

A 33-residue peptide tag increases solubility and stability of Escherichia coli produced single-chain antibody fragments

Low solubility and stability of Escherichia coli produced single chain variable fragments (scFvs) restrict their applications. Here the authors report a 33-residue peptide tag which simultaneously increases the solubility and thermostability of multiple scFvs produced in Escherichia coli SHuffle str...

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Bibliographic Details
Main Authors: Yang Wang, Wenjie Yuan, Siqi Guo, Qiqi Li, Xiaomei Chen, Cheng Li, Qianying Liu, Lei Sun, Zhenguo Chen, Zhenghong Yuan, Cheng Luo, Shijie Chen, Shuping Tong, Michael Nassal, Yu-Mei Wen, Yong-Xiang Wang
Format: Article
Language:English
Published: Nature Portfolio 2022-08-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-022-32423-9

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