FAST AND EFFICIENT METHOD FOR THE DETERMINATION OF LYSOZYME FROM GALLERIA MELLONELLA BIOMASS
The enzyme lysozyme has wide application in food and health industry due to its antimicrobial properties. Different alternative sources are used for the extraction lysozyme. One such alternative source ca be Galleria mellonella biomass, the larva of which can digest polyethylene. The method was deve...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
AcademicPres
2022-10-01
|
| Series: | Agricultura |
| Online Access: | https://journals.usamvcluj.ro/index.php/agricultura/article/view/14335 |
| _version_ | 1797768758342713344 |
|---|---|
| author | Anca Becze Mihai Naghiu |
| author_facet | Anca Becze Mihai Naghiu |
| author_sort | Anca Becze |
| collection | DOAJ |
| description | The enzyme lysozyme has wide application in food and health industry due to its antimicrobial properties. Different alternative sources are used for the extraction lysozyme. One such alternative source ca be Galleria mellonella biomass, the larva of which can digest polyethylene. The method was developed using an ultra-high pressure liquid chromatograph Vanquish H from Dionex, with fluorescent detector. The quantification limit obtained was 0.1 ng/ml and the recovery after solid/liquid extraction was 87.3 %. The samples were extracted using 30 ml of 33% NaCl. The extracted was purified using 0.5 g Florisil column. The mobile phase was a mixture of water and acetonitrile with 0.1 % trifluoro acetic acid. Very small quantities of lysozyme were found in the Galleria mellonella biomass. |
| first_indexed | 2024-03-12T20:57:55Z |
| format | Article |
| id | doaj.art-49e87d0589224c528f26f0b676d5b515 |
| institution | Directory Open Access Journal |
| issn | 1221-5317 |
| language | English |
| last_indexed | 2024-03-12T20:57:55Z |
| publishDate | 2022-10-01 |
| publisher | AcademicPres |
| record_format | Article |
| series | Agricultura |
| spelling | doaj.art-49e87d0589224c528f26f0b676d5b5152023-07-31T11:44:41ZengAcademicPresAgricultura1221-53172022-10-011203-4475014335FAST AND EFFICIENT METHOD FOR THE DETERMINATION OF LYSOZYME FROM GALLERIA MELLONELLA BIOMASSAnca BeczeMihai NaghiuThe enzyme lysozyme has wide application in food and health industry due to its antimicrobial properties. Different alternative sources are used for the extraction lysozyme. One such alternative source ca be Galleria mellonella biomass, the larva of which can digest polyethylene. The method was developed using an ultra-high pressure liquid chromatograph Vanquish H from Dionex, with fluorescent detector. The quantification limit obtained was 0.1 ng/ml and the recovery after solid/liquid extraction was 87.3 %. The samples were extracted using 30 ml of 33% NaCl. The extracted was purified using 0.5 g Florisil column. The mobile phase was a mixture of water and acetonitrile with 0.1 % trifluoro acetic acid. Very small quantities of lysozyme were found in the Galleria mellonella biomass.https://journals.usamvcluj.ro/index.php/agricultura/article/view/14335 |
| spellingShingle | Anca Becze Mihai Naghiu FAST AND EFFICIENT METHOD FOR THE DETERMINATION OF LYSOZYME FROM GALLERIA MELLONELLA BIOMASS Agricultura |
| title | FAST AND EFFICIENT METHOD FOR THE DETERMINATION OF LYSOZYME FROM GALLERIA MELLONELLA BIOMASS |
| title_full | FAST AND EFFICIENT METHOD FOR THE DETERMINATION OF LYSOZYME FROM GALLERIA MELLONELLA BIOMASS |
| title_fullStr | FAST AND EFFICIENT METHOD FOR THE DETERMINATION OF LYSOZYME FROM GALLERIA MELLONELLA BIOMASS |
| title_full_unstemmed | FAST AND EFFICIENT METHOD FOR THE DETERMINATION OF LYSOZYME FROM GALLERIA MELLONELLA BIOMASS |
| title_short | FAST AND EFFICIENT METHOD FOR THE DETERMINATION OF LYSOZYME FROM GALLERIA MELLONELLA BIOMASS |
| title_sort | fast and efficient method for the determination of lysozyme from galleria mellonella biomass |
| url | https://journals.usamvcluj.ro/index.php/agricultura/article/view/14335 |
| work_keys_str_mv | AT ancabecze fastandefficientmethodforthedeterminationoflysozymefromgalleriamellonellabiomass AT mihainaghiu fastandefficientmethodforthedeterminationoflysozymefromgalleriamellonellabiomass |