The role of exochitinase type A1 in the fungistatic activity of the rhizosphere bacterium Paenibacillus sp. M4
The aim of the study was to detect the activity and characterize potentially fungistatic chitinases synthesized by rhizosphere bacteria identified as Paenibacillus sp. M4. Maximum chitinolytic activity was achieved on the fifth day of culturing bacteria in a growth medium with 1% colloidal...
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2016-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2016/0354-46641500138J.pdf |
| _version_ | 1818846233257574400 |
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| author | Jankiewicz Urszula Swiontek-Brzezinska Maria |
| author_facet | Jankiewicz Urszula Swiontek-Brzezinska Maria |
| author_sort | Jankiewicz Urszula |
| collection | DOAJ |
| description | The aim of the study was to detect the activity and characterize potentially
fungistatic chitinases synthesized by rhizosphere bacteria identified as
Paenibacillus sp. M4. Maximum chitinolytic activity was achieved on the fifth
day of culturing bacteria in a growth medium with 1% colloidal chitin.
Analysis of a zymogram uncovered the presence of four activity bands in the
crude bacterial extract. The used three-stage protein purification procedure
resulted in a single band of chitinase activity on the zymogram. The purified
enzyme exhibited maximum activity at pH 6.5 and temperature 45oC, and thermal
stability at 40oC for 4 h. In terms of substrate specificity, it is an
exochitinase (chitobiose). The amino acid sequence obtained after mass
spectrometry showed similarity to chitinase A1 synthesized by Bacillus
circulans. The M4 isolate demonstrated the highest growth inhibiting activity
against plant pathogens belonging to the genera Fusarium, Rhizoctonia and
Alternaria. Fungistatic activity, although to a somewhat lesser degree, was
also demonstrated by purified chitinase. The obtained results confirm the
participation of the studied exochitinase in antagonism towards pathogenic
molds. However, the lower fungistatic effectiveness of the chitinases points
to the synergistic action of different metabolites in biocontrol by these
bacteria. |
| first_indexed | 2024-12-19T05:42:18Z |
| format | Article |
| id | doaj.art-49e8ce79434543c99d15146752feb922 |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-12-19T05:42:18Z |
| publishDate | 2016-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-49e8ce79434543c99d15146752feb9222022-12-21T20:33:59ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392016-01-0168245145910.2298/ABS150619138J0354-46641500138JThe role of exochitinase type A1 in the fungistatic activity of the rhizosphere bacterium Paenibacillus sp. M4Jankiewicz Urszula0Swiontek-Brzezinska Maria1Warsaw University of Life Sciences, Department of Biochemistry, SGGW, Warsaw, PolandNicolaus Copernicus University, Institute of Ecology and Environmental Protection, Department of Environmental Microbiology and Biotechnology, Torun, PolandThe aim of the study was to detect the activity and characterize potentially fungistatic chitinases synthesized by rhizosphere bacteria identified as Paenibacillus sp. M4. Maximum chitinolytic activity was achieved on the fifth day of culturing bacteria in a growth medium with 1% colloidal chitin. Analysis of a zymogram uncovered the presence of four activity bands in the crude bacterial extract. The used three-stage protein purification procedure resulted in a single band of chitinase activity on the zymogram. The purified enzyme exhibited maximum activity at pH 6.5 and temperature 45oC, and thermal stability at 40oC for 4 h. In terms of substrate specificity, it is an exochitinase (chitobiose). The amino acid sequence obtained after mass spectrometry showed similarity to chitinase A1 synthesized by Bacillus circulans. The M4 isolate demonstrated the highest growth inhibiting activity against plant pathogens belonging to the genera Fusarium, Rhizoctonia and Alternaria. Fungistatic activity, although to a somewhat lesser degree, was also demonstrated by purified chitinase. The obtained results confirm the participation of the studied exochitinase in antagonism towards pathogenic molds. However, the lower fungistatic effectiveness of the chitinases points to the synergistic action of different metabolites in biocontrol by these bacteria.http://www.doiserbia.nb.rs/img/doi/0354-4664/2016/0354-46641500138J.pdfchitinase purificationbiocontrolplant pathogensPaenibacillus sp.fungistatic activity |
| spellingShingle | Jankiewicz Urszula Swiontek-Brzezinska Maria The role of exochitinase type A1 in the fungistatic activity of the rhizosphere bacterium Paenibacillus sp. M4 Archives of Biological Sciences chitinase purification biocontrol plant pathogens Paenibacillus sp. fungistatic activity |
| title | The role of exochitinase type A1 in the fungistatic activity of the rhizosphere bacterium Paenibacillus sp. M4 |
| title_full | The role of exochitinase type A1 in the fungistatic activity of the rhizosphere bacterium Paenibacillus sp. M4 |
| title_fullStr | The role of exochitinase type A1 in the fungistatic activity of the rhizosphere bacterium Paenibacillus sp. M4 |
| title_full_unstemmed | The role of exochitinase type A1 in the fungistatic activity of the rhizosphere bacterium Paenibacillus sp. M4 |
| title_short | The role of exochitinase type A1 in the fungistatic activity of the rhizosphere bacterium Paenibacillus sp. M4 |
| title_sort | role of exochitinase type a1 in the fungistatic activity of the rhizosphere bacterium paenibacillus sp m4 |
| topic | chitinase purification biocontrol plant pathogens Paenibacillus sp. fungistatic activity |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2016/0354-46641500138J.pdf |
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