Inhibition of BDNF-AS Provides Neuroprotection for Retinal Ganglion Cells against Ischemic Injury.
Brain-derived neurotrophic factor (BDNF) protects retinal ganglion cells against ischemia in ocular degenerative diseases. We aimed to determine the effect of BDNF-AS on the ischemic injury of retinal ganglion cells.The levels of BDNF and BDNF-AS were measured in retinal ganglion cells subjected to...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5147780?pdf=render |
| _version_ | 1830519232992903168 |
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| author | Lifang Xu Ziyin Zhang Tianhua Xie Xiaoyang Zhang Tu Dai |
| author_facet | Lifang Xu Ziyin Zhang Tianhua Xie Xiaoyang Zhang Tu Dai |
| author_sort | Lifang Xu |
| collection | DOAJ |
| description | Brain-derived neurotrophic factor (BDNF) protects retinal ganglion cells against ischemia in ocular degenerative diseases. We aimed to determine the effect of BDNF-AS on the ischemic injury of retinal ganglion cells.The levels of BDNF and BDNF-AS were measured in retinal ganglion cells subjected to oxygen and glucose deprivation. The lentiviral vectors were constructed to either overexpress or knock out BDNF-AS. The luciferase reporter gene assay was used to determine whether BDNF-AS could target its seed sequence on BDNF mRNA. The methyl thiazolyl tetrazolium assay was used to determine cell viability, and TUNEL staining was used for cell apoptosis.The levels of BDNF-AS were negatively correlated with BDNF in ischemic retinal ganglion cells. BDNF-AS directly targeted its complementary sequences on BDNF mRNA. BDNF-AS regulated the expression of BDNF and its related genes in retinal ganglion cells. Down-regulation of BDNF-AS increased cell viability and decreased the number of TUNEL-positive retinal ganglion cells under oxygen and glucose deprivation conditions.Inhibition of BDNF-AS protected retinal ganglion cells against ischemia by increasing the levels of BDNF. |
| first_indexed | 2024-12-22T04:33:30Z |
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| id | doaj.art-49e941a5dbe7482baae98442525d4856 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T04:33:30Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
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| spelling | doaj.art-49e941a5dbe7482baae98442525d48562022-12-21T18:38:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-011112e016494110.1371/journal.pone.0164941Inhibition of BDNF-AS Provides Neuroprotection for Retinal Ganglion Cells against Ischemic Injury.Lifang XuZiyin ZhangTianhua XieXiaoyang ZhangTu DaiBrain-derived neurotrophic factor (BDNF) protects retinal ganglion cells against ischemia in ocular degenerative diseases. We aimed to determine the effect of BDNF-AS on the ischemic injury of retinal ganglion cells.The levels of BDNF and BDNF-AS were measured in retinal ganglion cells subjected to oxygen and glucose deprivation. The lentiviral vectors were constructed to either overexpress or knock out BDNF-AS. The luciferase reporter gene assay was used to determine whether BDNF-AS could target its seed sequence on BDNF mRNA. The methyl thiazolyl tetrazolium assay was used to determine cell viability, and TUNEL staining was used for cell apoptosis.The levels of BDNF-AS were negatively correlated with BDNF in ischemic retinal ganglion cells. BDNF-AS directly targeted its complementary sequences on BDNF mRNA. BDNF-AS regulated the expression of BDNF and its related genes in retinal ganglion cells. Down-regulation of BDNF-AS increased cell viability and decreased the number of TUNEL-positive retinal ganglion cells under oxygen and glucose deprivation conditions.Inhibition of BDNF-AS protected retinal ganglion cells against ischemia by increasing the levels of BDNF.http://europepmc.org/articles/PMC5147780?pdf=render |
| spellingShingle | Lifang Xu Ziyin Zhang Tianhua Xie Xiaoyang Zhang Tu Dai Inhibition of BDNF-AS Provides Neuroprotection for Retinal Ganglion Cells against Ischemic Injury. PLoS ONE |
| title | Inhibition of BDNF-AS Provides Neuroprotection for Retinal Ganglion Cells against Ischemic Injury. |
| title_full | Inhibition of BDNF-AS Provides Neuroprotection for Retinal Ganglion Cells against Ischemic Injury. |
| title_fullStr | Inhibition of BDNF-AS Provides Neuroprotection for Retinal Ganglion Cells against Ischemic Injury. |
| title_full_unstemmed | Inhibition of BDNF-AS Provides Neuroprotection for Retinal Ganglion Cells against Ischemic Injury. |
| title_short | Inhibition of BDNF-AS Provides Neuroprotection for Retinal Ganglion Cells against Ischemic Injury. |
| title_sort | inhibition of bdnf as provides neuroprotection for retinal ganglion cells against ischemic injury |
| url | http://europepmc.org/articles/PMC5147780?pdf=render |
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